Reduced Graphene Oxide-Based Impedimetric Immunosensor for Detection of Enterotoxin A in Milk Samples

• Main Authors: Giovanna S. Rocha, Martin K. L. Silva, Ivana Cesarino
• Format: Article
• Language: English
• Published: MDPI AG 2020-04-01
• Series: Materials
• Subjects: reduced graphene oxide; enterotoxin A; <i>Staphylococcus aureus</i>; impedimetric immunosensor
• Online Access: https://www.mdpi.com/1996-1944/13/7/1751

A simple, cheap, and less aggressive immobilization procedure for biomolecules using reduced graphene oxide (rGO) was employed to prepare an impedimetric immunosensor for detection of staphylococcal enterotoxin A (SEA) from <i>Staphylococcus aureus</i> in milk samples. The scanning electron microscopy, cyclic voltammetry, and electrochemical impedance spectroscopy (EIS) were used to monitor the single steps of the electrode assembly process. The glassy carbon (GC)/rGO platform detected the antigen-antibody binding procedures of SEA with concentrations of 0.5 to 3.5 mg L⁻¹ via impedance changes in a low frequency range. The impedimetric immunosensor was successfully applied for the determination of SEA in milk samples.