Reduced Graphene Oxide-Based Impedimetric Immunosensor for Detection of Enterotoxin A in Milk Samples
A simple, cheap, and less aggressive immobilization procedure for biomolecules using reduced graphene oxide (rGO) was employed to prepare an impedimetric immunosensor for detection of staphylococcal enterotoxin A (SEA) from <i>Staphylococcus aureus</i> in milk samples. The scanning elect...
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doaj-b3a653558b814efeab1ca344155f8b222020-11-25T02:33:00ZengMDPI AGMaterials1996-19442020-04-01131751175110.3390/ma13071751Reduced Graphene Oxide-Based Impedimetric Immunosensor for Detection of Enterotoxin A in Milk SamplesGiovanna S. Rocha0Martin K. L. Silva1Ivana Cesarino2Department of Bioprocesses and Biotechnology, School of Agriculture, São Paulo State University (UNESP), Botucatu 18610-034, BrazilDepartment of Bioprocesses and Biotechnology, School of Agriculture, São Paulo State University (UNESP), Botucatu 18610-034, BrazilDepartment of Bioprocesses and Biotechnology, School of Agriculture, São Paulo State University (UNESP), Botucatu 18610-034, BrazilA simple, cheap, and less aggressive immobilization procedure for biomolecules using reduced graphene oxide (rGO) was employed to prepare an impedimetric immunosensor for detection of staphylococcal enterotoxin A (SEA) from <i>Staphylococcus aureus</i> in milk samples. The scanning electron microscopy, cyclic voltammetry, and electrochemical impedance spectroscopy (EIS) were used to monitor the single steps of the electrode assembly process. The glassy carbon (GC)/rGO platform detected the antigen-antibody binding procedures of SEA with concentrations of 0.5 to 3.5 mg L<sup>−1</sup> via impedance changes in a low frequency range. The impedimetric immunosensor was successfully applied for the determination of SEA in milk samples.https://www.mdpi.com/1996-1944/13/7/1751reduced graphene oxideenterotoxin A<i>Staphylococcus aureus</i>impedimetric immunosensor |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Giovanna S. Rocha Martin K. L. Silva Ivana Cesarino |
spellingShingle |
Giovanna S. Rocha Martin K. L. Silva Ivana Cesarino Reduced Graphene Oxide-Based Impedimetric Immunosensor for Detection of Enterotoxin A in Milk Samples Materials reduced graphene oxide enterotoxin A <i>Staphylococcus aureus</i> impedimetric immunosensor |
author_facet |
Giovanna S. Rocha Martin K. L. Silva Ivana Cesarino |
author_sort |
Giovanna S. Rocha |
title |
Reduced Graphene Oxide-Based Impedimetric Immunosensor for Detection of Enterotoxin A in Milk Samples |
title_short |
Reduced Graphene Oxide-Based Impedimetric Immunosensor for Detection of Enterotoxin A in Milk Samples |
title_full |
Reduced Graphene Oxide-Based Impedimetric Immunosensor for Detection of Enterotoxin A in Milk Samples |
title_fullStr |
Reduced Graphene Oxide-Based Impedimetric Immunosensor for Detection of Enterotoxin A in Milk Samples |
title_full_unstemmed |
Reduced Graphene Oxide-Based Impedimetric Immunosensor for Detection of Enterotoxin A in Milk Samples |
title_sort |
reduced graphene oxide-based impedimetric immunosensor for detection of enterotoxin a in milk samples |
publisher |
MDPI AG |
series |
Materials |
issn |
1996-1944 |
publishDate |
2020-04-01 |
description |
A simple, cheap, and less aggressive immobilization procedure for biomolecules using reduced graphene oxide (rGO) was employed to prepare an impedimetric immunosensor for detection of staphylococcal enterotoxin A (SEA) from <i>Staphylococcus aureus</i> in milk samples. The scanning electron microscopy, cyclic voltammetry, and electrochemical impedance spectroscopy (EIS) were used to monitor the single steps of the electrode assembly process. The glassy carbon (GC)/rGO platform detected the antigen-antibody binding procedures of SEA with concentrations of 0.5 to 3.5 mg L<sup>−1</sup> via impedance changes in a low frequency range. The impedimetric immunosensor was successfully applied for the determination of SEA in milk samples. |
topic |
reduced graphene oxide enterotoxin A <i>Staphylococcus aureus</i> impedimetric immunosensor |
url |
https://www.mdpi.com/1996-1944/13/7/1751 |
work_keys_str_mv |
AT giovannasrocha reducedgrapheneoxidebasedimpedimetricimmunosensorfordetectionofenterotoxinainmilksamples AT martinklsilva reducedgrapheneoxidebasedimpedimetricimmunosensorfordetectionofenterotoxinainmilksamples AT ivanacesarino reducedgrapheneoxidebasedimpedimetricimmunosensorfordetectionofenterotoxinainmilksamples |
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