The Mesentery as a Laminated Vascular Bed for Hepatocyte Transplantation
The small bowel mesentery provides a unique structure of a large vascularized surface area to support hepatocyte transplantation. Cell-seeded polymeric matrices can be juxtaposed in a relatively atraumatic manner between leaves of mesentery such that adequate exchange of nutrients and diffusion of g...
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1994-07-01
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doaj-b3d44d1c55ed4f81a77ace545c4cc8862020-11-25T03:16:20ZengSAGE PublishingCell Transplantation0963-68971555-38921994-07-01310.1177/096368979400300403The Mesentery as a Laminated Vascular Bed for Hepatocyte TransplantationLynt B. Johnson M.D.0John Aiken1David Mooney2Betsy L. Schloo3Linda Griffith-Cima4Robert Langer5Joseph P. Vacanti6Department of Surgery, The Children's Hospital, Boston, MADepartment of Surgery, University of Cincinnati Medical Center, Cincinnati, OHDepartment of Chemical Engineering, Massachusetts Institute of Technology, Boston, MADepartment of Pathology, Deborah Heart and Lung Center, Brown Mills, NJDepartment of Pathology, Deborah Heart and Lung Center, Brown Mills, NJDepartment of Pathology, Deborah Heart and Lung Center, Brown Mills, NJDepartment of Surgery, The Children's Hospital, Boston, MAThe small bowel mesentery provides a unique structure of a large vascularized surface area to support hepatocyte transplantation. Cell-seeded polymeric matrices can be juxtaposed in a relatively atraumatic manner between leaves of mesentery such that adequate exchange of nutrients and diffusion of gases can proceed in the interim while neovascularization occurs. Hepatocytes obtained from (RHA) Wistar rats by collagenase perfusion were seeded onto non-woven filamentous sheets of polyglycolic acid 1 × 3 cm in size and 2 mm thickness to a density of 500,000 cells/cm2. Twenty-six recipient Gunn rats (UDP-glucuronyl transferase deficient) underwent laparotomy. Hepatocyte-ladened polymer sheets were placed between leaves of mesentery. Eight sheets were placed per animal and the leaves were approximated, creating a functional implant 1 × 3 × 2 cm. Biopsies between 5-99 days after implantation revealed neovascularization, moderate inflammatory reaction and the presence of viable hepatocytes in 96% (25/26). Immunoperoxidase studies using anti-albumin antibody substantiated hepatocyte specific function in implants. HPLC profiles of bile from Gunn rats transplanted with hepatocytes from congeneic (RHA) rats demonstrated the presence of bilirubin conjugates. There were no conjugation fractions seen in control gunn rats without hepatocyte transplantation. Although total serum bilirubin did not significantly decrease, conjugated bilirubin was identified in 46% (12/26) animals after transplantation with congeneic hepatocytes. We conclude that the mesentery of the small bowel provides a large vascularized surface for cell transplantation. Large numbers of metabolically active hepatocytes can engraft, vascularize, and show function. The mesentery may be a potential bed for clinical hepatocyte transplantation.https://doi.org/10.1177/096368979400300403 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lynt B. Johnson M.D. John Aiken David Mooney Betsy L. Schloo Linda Griffith-Cima Robert Langer Joseph P. Vacanti |
spellingShingle |
Lynt B. Johnson M.D. John Aiken David Mooney Betsy L. Schloo Linda Griffith-Cima Robert Langer Joseph P. Vacanti The Mesentery as a Laminated Vascular Bed for Hepatocyte Transplantation Cell Transplantation |
author_facet |
Lynt B. Johnson M.D. John Aiken David Mooney Betsy L. Schloo Linda Griffith-Cima Robert Langer Joseph P. Vacanti |
author_sort |
Lynt B. Johnson M.D. |
title |
The Mesentery as a Laminated Vascular Bed for Hepatocyte Transplantation |
title_short |
The Mesentery as a Laminated Vascular Bed for Hepatocyte Transplantation |
title_full |
The Mesentery as a Laminated Vascular Bed for Hepatocyte Transplantation |
title_fullStr |
The Mesentery as a Laminated Vascular Bed for Hepatocyte Transplantation |
title_full_unstemmed |
The Mesentery as a Laminated Vascular Bed for Hepatocyte Transplantation |
title_sort |
mesentery as a laminated vascular bed for hepatocyte transplantation |
publisher |
SAGE Publishing |
series |
Cell Transplantation |
issn |
0963-6897 1555-3892 |
publishDate |
1994-07-01 |
description |
The small bowel mesentery provides a unique structure of a large vascularized surface area to support hepatocyte transplantation. Cell-seeded polymeric matrices can be juxtaposed in a relatively atraumatic manner between leaves of mesentery such that adequate exchange of nutrients and diffusion of gases can proceed in the interim while neovascularization occurs. Hepatocytes obtained from (RHA) Wistar rats by collagenase perfusion were seeded onto non-woven filamentous sheets of polyglycolic acid 1 × 3 cm in size and 2 mm thickness to a density of 500,000 cells/cm2. Twenty-six recipient Gunn rats (UDP-glucuronyl transferase deficient) underwent laparotomy. Hepatocyte-ladened polymer sheets were placed between leaves of mesentery. Eight sheets were placed per animal and the leaves were approximated, creating a functional implant 1 × 3 × 2 cm. Biopsies between 5-99 days after implantation revealed neovascularization, moderate inflammatory reaction and the presence of viable hepatocytes in 96% (25/26). Immunoperoxidase studies using anti-albumin antibody substantiated hepatocyte specific function in implants. HPLC profiles of bile from Gunn rats transplanted with hepatocytes from congeneic (RHA) rats demonstrated the presence of bilirubin conjugates. There were no conjugation fractions seen in control gunn rats without hepatocyte transplantation. Although total serum bilirubin did not significantly decrease, conjugated bilirubin was identified in 46% (12/26) animals after transplantation with congeneic hepatocytes. We conclude that the mesentery of the small bowel provides a large vascularized surface for cell transplantation. Large numbers of metabolically active hepatocytes can engraft, vascularize, and show function. The mesentery may be a potential bed for clinical hepatocyte transplantation. |
url |
https://doi.org/10.1177/096368979400300403 |
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