Generation of a KLF15 homozygous knockout human embryonic stem cell line using paired CRISPR/Cas9n, and human cardiomyocytes derivation

Generation of a KLF15 homozygous knockout human embryonic stem cell line using paired CRISPR/Cas9n, and human cardiomyocytes derivation

Krueppel-like factor 15 (KLF15) is abundantly expressed in liver, kidney, and muscle, including myocardium. In the adult heart KLF15 is important to maintain homeostasis and to repress hypertrophic remodeling. We generated a homozygous hESC KLF15 knockout (KO) line using paired CRISPR/Cas9n. KLF15-K...

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Main Authors: Claudia Noack, Luis Peter Haupt, Wolfram-Hubertus Zimmermann, Katrin Streckfuss-Bömeke, Laura Cecilia Zelarayán
Format: Article
Language:English
Published: Elsevier 2017-08-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506117301319
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spelling doaj-b3d4ee37248248eeaf0f489e459b5be22020-11-24T23:51:15ZengElsevierStem Cell Research1873-50611876-77532017-08-0123C12713110.1016/j.scr.2017.07.007Generation of a KLF15 homozygous knockout human embryonic stem cell line using paired CRISPR/Cas9n, and human cardiomyocytes derivationClaudia Noack0Luis Peter Haupt1Wolfram-Hubertus Zimmermann2Katrin Streckfuss-Bömeke3Laura Cecilia Zelarayán4Institute of Pharmacology and Toxicology, University Medical Center Goettingen, GermanyClinic for Cardiology and Pneumology, University Medical Center Goettingen, GermanyInstitute of Pharmacology and Toxicology, University Medical Center Goettingen, GermanyClinic for Cardiology and Pneumology, University Medical Center Goettingen, GermanyInstitute of Pharmacology and Toxicology, University Medical Center Goettingen, GermanyKrueppel-like factor 15 (KLF15) is abundantly expressed in liver, kidney, and muscle, including myocardium. In the adult heart KLF15 is important to maintain homeostasis and to repress hypertrophic remodeling. We generated a homozygous hESC KLF15 knockout (KO) line using paired CRISPR/Cas9n. KLF15-KO cells maintained full pluripotency and differentiation potential as well as genomic integrity. We demonstrated that KLF15-KO cells can be differentiated into morphologically normal cardiomyocytes turning them into a valuable tool for studying human KLF15-mediated mechanisms resulting in human cardiac dysfunction.http://www.sciencedirect.com/science/article/pii/S1873506117301319
collection DOAJ
language English
format Article
sources DOAJ
author Claudia Noack
Luis Peter Haupt
Wolfram-Hubertus Zimmermann
Katrin Streckfuss-Bömeke
Laura Cecilia Zelarayán
spellingShingle Claudia Noack
Luis Peter Haupt
Wolfram-Hubertus Zimmermann
Katrin Streckfuss-Bömeke
Laura Cecilia Zelarayán
Generation of a KLF15 homozygous knockout human embryonic stem cell line using paired CRISPR/Cas9n, and human cardiomyocytes derivation
Stem Cell Research
author_facet Claudia Noack
Luis Peter Haupt
Wolfram-Hubertus Zimmermann
Katrin Streckfuss-Bömeke
Laura Cecilia Zelarayán
author_sort Claudia Noack
title Generation of a KLF15 homozygous knockout human embryonic stem cell line using paired CRISPR/Cas9n, and human cardiomyocytes derivation
title_short Generation of a KLF15 homozygous knockout human embryonic stem cell line using paired CRISPR/Cas9n, and human cardiomyocytes derivation
title_full Generation of a KLF15 homozygous knockout human embryonic stem cell line using paired CRISPR/Cas9n, and human cardiomyocytes derivation
title_fullStr Generation of a KLF15 homozygous knockout human embryonic stem cell line using paired CRISPR/Cas9n, and human cardiomyocytes derivation
title_full_unstemmed Generation of a KLF15 homozygous knockout human embryonic stem cell line using paired CRISPR/Cas9n, and human cardiomyocytes derivation
title_sort generation of a klf15 homozygous knockout human embryonic stem cell line using paired crispr/cas9n, and human cardiomyocytes derivation
publisher Elsevier
series Stem Cell Research
issn 1873-5061
1876-7753
publishDate 2017-08-01
description Krueppel-like factor 15 (KLF15) is abundantly expressed in liver, kidney, and muscle, including myocardium. In the adult heart KLF15 is important to maintain homeostasis and to repress hypertrophic remodeling. We generated a homozygous hESC KLF15 knockout (KO) line using paired CRISPR/Cas9n. KLF15-KO cells maintained full pluripotency and differentiation potential as well as genomic integrity. We demonstrated that KLF15-KO cells can be differentiated into morphologically normal cardiomyocytes turning them into a valuable tool for studying human KLF15-mediated mechanisms resulting in human cardiac dysfunction.
url http://www.sciencedirect.com/science/article/pii/S1873506117301319
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