A fraction from Petiveria alliacea induces apoptosis via a mitochondria-dependent pathway and regulates HSP70 expression

A fraction from Petiveria alliacea induces apoptosis via a mitochondria-dependent pathway and regulates HSP70 expression

To evaluate the biological activity of Petiveria alliacea extracts on tumoral cells in vitro. Materials and methods. P.alliaceafractions prepared by a bioguided purification protocol were characterized by their biological activities on two human tumoral cell lines.Morphological changes, cell viabili...

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Main Authors: Susana Fiorentino, Diana Mercedes Castañeda, Claudia Patricia Urueña, Maria Claudia Cifuentes
Format: Article
Language:English
Published: Pontificia Universidad Javeriana 2009-12-01
Series:Universitas Scientiarum
Subjects:
Apoptosis
cancer
heat shock proteins
Petiveria alliacea
Online Access:http://revistas.javeriana.edu.co/index.php/scientarium/article/view/1382/845
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spelling doaj-b3dd65e9ab944cf0a36936007bb24ebe2020-11-24T23:29:29ZengPontificia Universidad JaverianaUniversitas Scientiarum0122-74832027-13522009-12-01142-3125134A fraction from Petiveria alliacea induces apoptosis via a mitochondria-dependent pathway and regulates HSP70 expressionSusana FiorentinoDiana Mercedes CastañedaClaudia Patricia UrueñaMaria Claudia CifuentesTo evaluate the biological activity of Petiveria alliacea extracts on tumoral cells in vitro. Materials and methods. P.alliaceafractions prepared by a bioguided purification protocol were characterized by their biological activities on two human tumoral cell lines.Morphological changes, cell viability, mitochondrial membrane depolarization, nuclear staining and activity on HSP70 were analyzed.Results. The present study demonstrates that P.alliacea fractions can induce apoptosis in a mitochondria-dependent pathway and downregulate HSP70 expression in vitro. The possible compounds present in P.alliacea responsible for the described biological activities wereidentified by dereplication methods. Conclusion. The present study provides new knowledge on the antitumoral properties of the plantspecies P. alliacea described in several ethnobotanical studies.http://revistas.javeriana.edu.co/index.php/scientarium/article/view/1382/845Apoptosiscancerheat shock proteinsPetiveria alliacea
collection DOAJ
language English
format Article
sources DOAJ
author Susana Fiorentino
Diana Mercedes Castañeda
Claudia Patricia Urueña
Maria Claudia Cifuentes
spellingShingle Susana Fiorentino
Diana Mercedes Castañeda
Claudia Patricia Urueña
Maria Claudia Cifuentes
A fraction from Petiveria alliacea induces apoptosis via a mitochondria-dependent pathway and regulates HSP70 expression
Universitas Scientiarum
Apoptosis
cancer
heat shock proteins
Petiveria alliacea
author_facet Susana Fiorentino
Diana Mercedes Castañeda
Claudia Patricia Urueña
Maria Claudia Cifuentes
author_sort Susana Fiorentino
title A fraction from Petiveria alliacea induces apoptosis via a mitochondria-dependent pathway and regulates HSP70 expression
title_short A fraction from Petiveria alliacea induces apoptosis via a mitochondria-dependent pathway and regulates HSP70 expression
title_full A fraction from Petiveria alliacea induces apoptosis via a mitochondria-dependent pathway and regulates HSP70 expression
title_fullStr A fraction from Petiveria alliacea induces apoptosis via a mitochondria-dependent pathway and regulates HSP70 expression
title_full_unstemmed A fraction from Petiveria alliacea induces apoptosis via a mitochondria-dependent pathway and regulates HSP70 expression
title_sort fraction from petiveria alliacea induces apoptosis via a mitochondria-dependent pathway and regulates hsp70 expression
publisher Pontificia Universidad Javeriana
series Universitas Scientiarum
issn 0122-7483
2027-1352
publishDate 2009-12-01
description To evaluate the biological activity of Petiveria alliacea extracts on tumoral cells in vitro. Materials and methods. P.alliaceafractions prepared by a bioguided purification protocol were characterized by their biological activities on two human tumoral cell lines.Morphological changes, cell viability, mitochondrial membrane depolarization, nuclear staining and activity on HSP70 were analyzed.Results. The present study demonstrates that P.alliacea fractions can induce apoptosis in a mitochondria-dependent pathway and downregulate HSP70 expression in vitro. The possible compounds present in P.alliacea responsible for the described biological activities wereidentified by dereplication methods. Conclusion. The present study provides new knowledge on the antitumoral properties of the plantspecies P. alliacea described in several ethnobotanical studies.
topic Apoptosis
cancer
heat shock proteins
Petiveria alliacea
url http://revistas.javeriana.edu.co/index.php/scientarium/article/view/1382/845
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