Evaluation of visible implant elastomer tags in zebrafish (Danio rerio)
Summary The use of the visible implant elastomer (VIE) tagging system in zebrafish (Danio rerio) was examined. Two tag orientations (horizontal and vertical) at the dorsal fin base were tested for tag retention, tag fragmentation and whether VIE tags affected growth and survival of juvenile zebrafis...
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2013-11-01
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doaj-b3e2419db31f432681e2f11022ed59012021-06-02T15:37:41ZengThe Company of BiologistsBiology Open2046-63902013-11-012121397140110.1242/bio.2013646020136460Evaluation of visible implant elastomer tags in zebrafish (Danio rerio)Claudia HohnLora Petrie-HansonSummary The use of the visible implant elastomer (VIE) tagging system in zebrafish (Danio rerio) was examined. Two tag orientations (horizontal and vertical) at the dorsal fin base were tested for tag retention, tag fragmentation and whether VIE tags affected growth and survival of juvenile zebrafish (1–4 month post hatch). Six tag locations (abdomen, anal fin base, caudal peduncle, dorsal fin base, pectoral fin base, isthmus) and 5 tag colors (yellow, red, pink, orange, blue) were evaluated for ease of VIE tag application and tag visibility in adult zebrafish. Long-term retention (1 year) and multiple tagging sites (right and left of dorsal fin and pectoral fin base) were examined in adult zebrafish. Lastly, survival of recombination activation gene 1−/− (rag1−/−) zebrafish was evaluated after VIE tagging. The best tag location was the dorsal fin base, and the most visible tag color was pink. Growth rate of juvenile zebrafish was not affected by VIE tagging. Horizontal tagging is recommended in early stages of fish growth (1–2 months post hatch). VIE tags were retained for 1 year and tagging did not interfere with long-term growth and survival. There was no mortality associated with VIE tagging in rag1−/− zebrafish. The VIE tagging system is highly suitable for small-sized zebrafish. When familiar with the procedure, 120 adult zebrafish can be tagged in one hour. It does not increase mortality in adult zebrafish or interfere with growth in juvenile or adult zebrafish.http://bio.biologists.org/content/2/12/1397IdentificationZebrafishDanio rerioVisible implant elastomerTaggingVIE tag |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Claudia Hohn Lora Petrie-Hanson |
spellingShingle |
Claudia Hohn Lora Petrie-Hanson Evaluation of visible implant elastomer tags in zebrafish (Danio rerio) Biology Open Identification Zebrafish Danio rerio Visible implant elastomer Tagging VIE tag |
author_facet |
Claudia Hohn Lora Petrie-Hanson |
author_sort |
Claudia Hohn |
title |
Evaluation of visible implant elastomer tags in zebrafish (Danio rerio) |
title_short |
Evaluation of visible implant elastomer tags in zebrafish (Danio rerio) |
title_full |
Evaluation of visible implant elastomer tags in zebrafish (Danio rerio) |
title_fullStr |
Evaluation of visible implant elastomer tags in zebrafish (Danio rerio) |
title_full_unstemmed |
Evaluation of visible implant elastomer tags in zebrafish (Danio rerio) |
title_sort |
evaluation of visible implant elastomer tags in zebrafish (danio rerio) |
publisher |
The Company of Biologists |
series |
Biology Open |
issn |
2046-6390 |
publishDate |
2013-11-01 |
description |
Summary
The use of the visible implant elastomer (VIE) tagging system in zebrafish (Danio rerio) was examined. Two tag orientations (horizontal and vertical) at the dorsal fin base were tested for tag retention, tag fragmentation and whether VIE tags affected growth and survival of juvenile zebrafish (1–4 month post hatch). Six tag locations (abdomen, anal fin base, caudal peduncle, dorsal fin base, pectoral fin base, isthmus) and 5 tag colors (yellow, red, pink, orange, blue) were evaluated for ease of VIE tag application and tag visibility in adult zebrafish. Long-term retention (1 year) and multiple tagging sites (right and left of dorsal fin and pectoral fin base) were examined in adult zebrafish. Lastly, survival of recombination activation gene 1−/− (rag1−/−) zebrafish was evaluated after VIE tagging. The best tag location was the dorsal fin base, and the most visible tag color was pink. Growth rate of juvenile zebrafish was not affected by VIE tagging. Horizontal tagging is recommended in early stages of fish growth (1–2 months post hatch). VIE tags were retained for 1 year and tagging did not interfere with long-term growth and survival. There was no mortality associated with VIE tagging in rag1−/− zebrafish. The VIE tagging system is highly suitable for small-sized zebrafish. When familiar with the procedure, 120 adult zebrafish can be tagged in one hour. It does not increase mortality in adult zebrafish or interfere with growth in juvenile or adult zebrafish. |
topic |
Identification Zebrafish Danio rerio Visible implant elastomer Tagging VIE tag |
url |
http://bio.biologists.org/content/2/12/1397 |
work_keys_str_mv |
AT claudiahohn evaluationofvisibleimplantelastomertagsinzebrafishdaniorerio AT lorapetriehanson evaluationofvisibleimplantelastomertagsinzebrafishdaniorerio |
_version_ |
1721403162063011840 |