Antibiotic resistance and molecular typing of Pseudomonas aeruginosa: focus on imipenem
Susceptibility tests by disk diffusion and by E-test and molecular typing by macrorestriction analysis were performed to determine the relatedness of Pseudomonas aeruginosa isolates from three distinct hospitals. The resistance profile of 124 isolates to 8 antimicrobial agents was determined in thre...
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doaj-b3f6cd09696a4c61890ddd6a9ec665df2020-11-25T03:48:42ZengElsevierBrazilian Journal of Infectious Diseases1413-86701678-43912002-01-01610106Antibiotic resistance and molecular typing of Pseudomonas aeruginosa: focus on imipenemFreitas Ana Lúcia Peixoto deBarth Afonso LuisSusceptibility tests by disk diffusion and by E-test and molecular typing by macrorestriction analysis were performed to determine the relatedness of Pseudomonas aeruginosa isolates from three distinct hospitals. The resistance profile of 124 isolates to 8 antimicrobial agents was determined in three different hospitals, in Porto Alegre, Brazil. Frequencies of susceptibility ranged from 43.9% for carbenicillin to 87.7% for ceftazidime. Cross-resistance data of imipenem-resistant isolates indicated that most (70%) were also resistant to carbenicillin, although 30% remained susceptible to ceftazidime and cefepime. In general, susceptibility profiles were not able to determine relatedness among isolates of P. aeruginosa. On the other hand, molecular typing by macrorestriction analysis demonstrated high discriminatory power and identified 66 strains among 72 isolates of P. aeruginosa. Imipenem-susceptible isolates were all different. However, identical clones of imipenem-resistant isolates were found in two of the hospitals, despite variable response to other antibiotics. No clustering of infection among the different medical centers was observed. In conclusion, clones of P. aeruginosa did not spread among the different hospitals in our city even though related isolates of imipenem-resistant P. aeruginosa were found.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702002000100001Pseudomonas aeruginosaantibiotic resistanceimipenem |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Freitas Ana Lúcia Peixoto de Barth Afonso Luis |
spellingShingle |
Freitas Ana Lúcia Peixoto de Barth Afonso Luis Antibiotic resistance and molecular typing of Pseudomonas aeruginosa: focus on imipenem Brazilian Journal of Infectious Diseases Pseudomonas aeruginosa antibiotic resistance imipenem |
author_facet |
Freitas Ana Lúcia Peixoto de Barth Afonso Luis |
author_sort |
Freitas Ana Lúcia Peixoto de |
title |
Antibiotic resistance and molecular typing of Pseudomonas aeruginosa: focus on imipenem |
title_short |
Antibiotic resistance and molecular typing of Pseudomonas aeruginosa: focus on imipenem |
title_full |
Antibiotic resistance and molecular typing of Pseudomonas aeruginosa: focus on imipenem |
title_fullStr |
Antibiotic resistance and molecular typing of Pseudomonas aeruginosa: focus on imipenem |
title_full_unstemmed |
Antibiotic resistance and molecular typing of Pseudomonas aeruginosa: focus on imipenem |
title_sort |
antibiotic resistance and molecular typing of pseudomonas aeruginosa: focus on imipenem |
publisher |
Elsevier |
series |
Brazilian Journal of Infectious Diseases |
issn |
1413-8670 1678-4391 |
publishDate |
2002-01-01 |
description |
Susceptibility tests by disk diffusion and by E-test and molecular typing by macrorestriction analysis were performed to determine the relatedness of Pseudomonas aeruginosa isolates from three distinct hospitals. The resistance profile of 124 isolates to 8 antimicrobial agents was determined in three different hospitals, in Porto Alegre, Brazil. Frequencies of susceptibility ranged from 43.9% for carbenicillin to 87.7% for ceftazidime. Cross-resistance data of imipenem-resistant isolates indicated that most (70%) were also resistant to carbenicillin, although 30% remained susceptible to ceftazidime and cefepime. In general, susceptibility profiles were not able to determine relatedness among isolates of P. aeruginosa. On the other hand, molecular typing by macrorestriction analysis demonstrated high discriminatory power and identified 66 strains among 72 isolates of P. aeruginosa. Imipenem-susceptible isolates were all different. However, identical clones of imipenem-resistant isolates were found in two of the hospitals, despite variable response to other antibiotics. No clustering of infection among the different medical centers was observed. In conclusion, clones of P. aeruginosa did not spread among the different hospitals in our city even though related isolates of imipenem-resistant P. aeruginosa were found. |
topic |
Pseudomonas aeruginosa antibiotic resistance imipenem |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702002000100001 |
work_keys_str_mv |
AT freitasanaluciapeixotode antibioticresistanceandmoleculartypingofpseudomonasaeruginosafocusonimipenem AT barthafonsoluis antibioticresistanceandmoleculartypingofpseudomonasaeruginosafocusonimipenem |
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