Quantitative volumetric Raman imaging of three dimensional cell cultures

Advances in chemical imaging capability can add to our understanding of complex cellular systems. Here the authors develop a framework for label-free quantitative volumetric Raman spectroscopic imaging and use it to visualize and quantify biomolecules in various 3D cellular systems.

Bibliographic Details
Main Authors:	Charalambos Kallepitis, Mads S. Bergholt, Manuel M. Mazo, Vincent Leonardo, Stacey C. Skaalure, Stephanie A. Maynard, Molly M. Stevens
Format:	Article
Language:	English
Published:	Nature Publishing Group 2017-03-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/ncomms14843

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spelling	doaj-b4178d40fab0467ea6e577c3dc89c9c42021-05-11T07:24:06ZengNature Publishing GroupNature Communications2041-17232017-03-01811910.1038/ncomms14843Quantitative volumetric Raman imaging of three dimensional cell culturesCharalambos Kallepitis0Mads S. Bergholt1Manuel M. Mazo2Vincent Leonardo3Stacey C. Skaalure4Stephanie A. Maynard5Molly M. Stevens6Department of Materials, Imperial College LondonDepartment of Materials, Imperial College LondonDepartment of Materials, Imperial College LondonDepartment of Materials, Imperial College LondonDepartment of Materials, Imperial College LondonDepartment of Materials, Imperial College LondonDepartment of Materials, Imperial College LondonAdvances in chemical imaging capability can add to our understanding of complex cellular systems. Here the authors develop a framework for label-free quantitative volumetric Raman spectroscopic imaging and use it to visualize and quantify biomolecules in various 3D cellular systems.https://doi.org/10.1038/ncomms14843
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Charalambos Kallepitis Mads S. Bergholt Manuel M. Mazo Vincent Leonardo Stacey C. Skaalure Stephanie A. Maynard Molly M. Stevens
spellingShingle	Charalambos Kallepitis Mads S. Bergholt Manuel M. Mazo Vincent Leonardo Stacey C. Skaalure Stephanie A. Maynard Molly M. Stevens Quantitative volumetric Raman imaging of three dimensional cell cultures Nature Communications
author_facet	Charalambos Kallepitis Mads S. Bergholt Manuel M. Mazo Vincent Leonardo Stacey C. Skaalure Stephanie A. Maynard Molly M. Stevens
author_sort	Charalambos Kallepitis
title	Quantitative volumetric Raman imaging of three dimensional cell cultures
title_short	Quantitative volumetric Raman imaging of three dimensional cell cultures
title_full	Quantitative volumetric Raman imaging of three dimensional cell cultures
title_fullStr	Quantitative volumetric Raman imaging of three dimensional cell cultures
title_full_unstemmed	Quantitative volumetric Raman imaging of three dimensional cell cultures
title_sort	quantitative volumetric raman imaging of three dimensional cell cultures
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2017-03-01
description	Advances in chemical imaging capability can add to our understanding of complex cellular systems. Here the authors develop a framework for label-free quantitative volumetric Raman spectroscopic imaging and use it to visualize and quantify biomolecules in various 3D cellular systems.
url	https://doi.org/10.1038/ncomms14843
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Quantitative volumetric Raman imaging of three dimensional cell cultures

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