PMab-235: A monoclonal antibody for immunohistochemical analysis against goat podoplanin
Sensitive and specific monoclonal antibodies (mAbs) against not only human but also mouse, rat, rabbit, dog, cat, bovine, pig, and horse podoplanins (PDPNs) have been established in our previous studies. However, anti-goat PDPN (gPDPN) has not been established yet. PDPN has been utilized as a lympha...
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doaj-b42f0838abc64e40bb38d787effe0f382020-11-25T02:14:03ZengElsevierHeliyon2405-84402019-07-0157e02063PMab-235: A monoclonal antibody for immunohistochemical analysis against goat podoplaninYoshikazu Furusawa0Shinji Yamada1Takuro Nakamura2Masato Sano3Yusuke Sayama4Shunsuke Itai5Junko Takei6Hiroyuki Harada7Masato Fukui8Mika K. Kaneko9Yukinari Kato10Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi, 980-8575, Japan; New Industry Creation Hatchery Center, Tohoku University, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi, 980-8575, Japan; ZENOAQ RESOURCE CO., LTD., 1-1 Tairanoue, Sasagawa, Asaka-machi, Koriyama, Fukushima, 963-0196, JapanDepartment of Antibody Drug Development, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi, 980-8575, JapanDepartment of Antibody Drug Development, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi, 980-8575, JapanDepartment of Antibody Drug Development, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi, 980-8575, JapanDepartment of Antibody Drug Development, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi, 980-8575, JapanDepartment of Antibody Drug Development, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi, 980-8575, Japan; Department of Oral and Maxillofacial Surgery, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo, 113-8510, JapanDepartment of Antibody Drug Development, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi, 980-8575, Japan; Department of Oral and Maxillofacial Surgery, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo, 113-8510, JapanDepartment of Oral and Maxillofacial Surgery, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo, 113-8510, JapanZENOAQ RESOURCE CO., LTD., 1-1 Tairanoue, Sasagawa, Asaka-machi, Koriyama, Fukushima, 963-0196, JapanDepartment of Antibody Drug Development, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi, 980-8575, JapanDepartment of Antibody Drug Development, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi, 980-8575, Japan; New Industry Creation Hatchery Center, Tohoku University, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi, 980-8575, Japan; Corresponding author.Sensitive and specific monoclonal antibodies (mAbs) against not only human but also mouse, rat, rabbit, dog, cat, bovine, pig, and horse podoplanins (PDPNs) have been established in our previous studies. However, anti-goat PDPN (gPDPN) has not been established yet. PDPN has been utilized as a lymphatic endothelial cell marker especially in pathological diagnoses; therefore, mAbs for immunohistochemical analyses using formalin-fixed paraffin-embedded tissues are needed. Although we recently demonstrated that an anti-bovine PDPN mAb, PMab-44 cross-reacted with gPDPN, PMab-44 did not detect lymphatic endothelial cells in immunohistochemistry. In this study, we immunized mice with gPDPN-overexpressing Chinese hamster ovary (CHO)–K1 (CHO/gPDPN) cells, and screened mAbs against gPDPN using flow cytometry. One of the mAbs, PMab-235 (IgG1, kappa), specifically detected CHO/gPDPN cells by flow cytometry. Furthermore, PMab-235 strongly detected lung type I alveolar cells, renal podocytes, and lymphatic endothelial cells of colon by immunohistochemistry. These findings suggest that PMab-235 may be useful as a lymphatic endothelial cell marker for goat tissues.http://www.sciencedirect.com/science/article/pii/S2405844019357238Lymphatic endothelial cellsPMab-235Goat podoplaninPDPN |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yoshikazu Furusawa Shinji Yamada Takuro Nakamura Masato Sano Yusuke Sayama Shunsuke Itai Junko Takei Hiroyuki Harada Masato Fukui Mika K. Kaneko Yukinari Kato |
spellingShingle |
Yoshikazu Furusawa Shinji Yamada Takuro Nakamura Masato Sano Yusuke Sayama Shunsuke Itai Junko Takei Hiroyuki Harada Masato Fukui Mika K. Kaneko Yukinari Kato PMab-235: A monoclonal antibody for immunohistochemical analysis against goat podoplanin Heliyon Lymphatic endothelial cells PMab-235 Goat podoplanin PDPN |
author_facet |
Yoshikazu Furusawa Shinji Yamada Takuro Nakamura Masato Sano Yusuke Sayama Shunsuke Itai Junko Takei Hiroyuki Harada Masato Fukui Mika K. Kaneko Yukinari Kato |
author_sort |
Yoshikazu Furusawa |
title |
PMab-235: A monoclonal antibody for immunohistochemical analysis against goat podoplanin |
title_short |
PMab-235: A monoclonal antibody for immunohistochemical analysis against goat podoplanin |
title_full |
PMab-235: A monoclonal antibody for immunohistochemical analysis against goat podoplanin |
title_fullStr |
PMab-235: A monoclonal antibody for immunohistochemical analysis against goat podoplanin |
title_full_unstemmed |
PMab-235: A monoclonal antibody for immunohistochemical analysis against goat podoplanin |
title_sort |
pmab-235: a monoclonal antibody for immunohistochemical analysis against goat podoplanin |
publisher |
Elsevier |
series |
Heliyon |
issn |
2405-8440 |
publishDate |
2019-07-01 |
description |
Sensitive and specific monoclonal antibodies (mAbs) against not only human but also mouse, rat, rabbit, dog, cat, bovine, pig, and horse podoplanins (PDPNs) have been established in our previous studies. However, anti-goat PDPN (gPDPN) has not been established yet. PDPN has been utilized as a lymphatic endothelial cell marker especially in pathological diagnoses; therefore, mAbs for immunohistochemical analyses using formalin-fixed paraffin-embedded tissues are needed. Although we recently demonstrated that an anti-bovine PDPN mAb, PMab-44 cross-reacted with gPDPN, PMab-44 did not detect lymphatic endothelial cells in immunohistochemistry. In this study, we immunized mice with gPDPN-overexpressing Chinese hamster ovary (CHO)–K1 (CHO/gPDPN) cells, and screened mAbs against gPDPN using flow cytometry. One of the mAbs, PMab-235 (IgG1, kappa), specifically detected CHO/gPDPN cells by flow cytometry. Furthermore, PMab-235 strongly detected lung type I alveolar cells, renal podocytes, and lymphatic endothelial cells of colon by immunohistochemistry. These findings suggest that PMab-235 may be useful as a lymphatic endothelial cell marker for goat tissues. |
topic |
Lymphatic endothelial cells PMab-235 Goat podoplanin PDPN |
url |
http://www.sciencedirect.com/science/article/pii/S2405844019357238 |
work_keys_str_mv |
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