Antibacterial Activity of Neat Chitosan Powder and Flakes
This study investigates the antibacterial activity of neat chitosan powder and flakes against three different bacterial species, Escherichia coli, Listeria innocua and Staphylococcus aureus, which are frequent causes of food spoilage. The effect of chitosan concentration and purity, as well as the i...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2017-01-01
|
Series: | Molecules |
Subjects: | |
Online Access: | http://www.mdpi.com/1420-3049/22/1/100 |
id |
doaj-b4505753e52f4fcc8bf54d29db0d75c5 |
---|---|
record_format |
Article |
spelling |
doaj-b4505753e52f4fcc8bf54d29db0d75c52020-11-24T22:44:08ZengMDPI AGMolecules1420-30492017-01-0122110010.3390/molecules22010100molecules22010100Antibacterial Activity of Neat Chitosan Powder and FlakesNury Ardila0France Daigle1Marie-Claude Heuzey2Abdellah Ajji3Research Center for High Performance Polymer and Composite Systems (CREPEC), Department of Chemical Engineering, Polytechnique Montréal, P.O. Box 6079, Station Centre-Ville, Montréal, QC H3C 3A7, CanadaDepartment of Microbiology, Infectiology and Immunology, Pavillon Roger-Gaudry, Université de Montréal, P.O. Box 6128, Station Centre-ville, Montréal, QC H3C 3J7, CanadaResearch Center for High Performance Polymer and Composite Systems (CREPEC), Department of Chemical Engineering, Polytechnique Montréal, P.O. Box 6079, Station Centre-Ville, Montréal, QC H3C 3A7, CanadaResearch Center for High Performance Polymer and Composite Systems (CREPEC), Department of Chemical Engineering, Polytechnique Montréal, P.O. Box 6079, Station Centre-Ville, Montréal, QC H3C 3A7, CanadaThis study investigates the antibacterial activity of neat chitosan powder and flakes against three different bacterial species, Escherichia coli, Listeria innocua and Staphylococcus aureus, which are frequent causes of food spoilage. The effect of chitosan concentration and purity, as well as the influence of temperature, ionic strength (salt) and impact of a solid physical support in the medium are examined. Results show that the antibacterial activity of neat chitosan: (i) requires partial solubilisation; (ii) can be promoted by environmental factors such as adequate temperature range, ionic strength and the presence of a solid physical support that may facilitate the attachment of bacteria; (iii) depends on bacterial species, with a sensitivity order of E. coli > L. innocua > S. aureus; and (iv) increases with chitosan concentration, up to a critical point above which this effect decreases. The latter may be due to remaining proteins in chitosan acting as nutrients for bacteria therefore limiting its antibacterial activity. These results on the direct use of chitosan powder and flakes as potential antimicrobial agents for food protection at pH values lower than the chitosan pKa (6.2–6.7) are promising.http://www.mdpi.com/1420-3049/22/1/100chitosanantibacterial activityE. coliL. innocuaS. aureus |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nury Ardila France Daigle Marie-Claude Heuzey Abdellah Ajji |
spellingShingle |
Nury Ardila France Daigle Marie-Claude Heuzey Abdellah Ajji Antibacterial Activity of Neat Chitosan Powder and Flakes Molecules chitosan antibacterial activity E. coli L. innocua S. aureus |
author_facet |
Nury Ardila France Daigle Marie-Claude Heuzey Abdellah Ajji |
author_sort |
Nury Ardila |
title |
Antibacterial Activity of Neat Chitosan Powder and Flakes |
title_short |
Antibacterial Activity of Neat Chitosan Powder and Flakes |
title_full |
Antibacterial Activity of Neat Chitosan Powder and Flakes |
title_fullStr |
Antibacterial Activity of Neat Chitosan Powder and Flakes |
title_full_unstemmed |
Antibacterial Activity of Neat Chitosan Powder and Flakes |
title_sort |
antibacterial activity of neat chitosan powder and flakes |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2017-01-01 |
description |
This study investigates the antibacterial activity of neat chitosan powder and flakes against three different bacterial species, Escherichia coli, Listeria innocua and Staphylococcus aureus, which are frequent causes of food spoilage. The effect of chitosan concentration and purity, as well as the influence of temperature, ionic strength (salt) and impact of a solid physical support in the medium are examined. Results show that the antibacterial activity of neat chitosan: (i) requires partial solubilisation; (ii) can be promoted by environmental factors such as adequate temperature range, ionic strength and the presence of a solid physical support that may facilitate the attachment of bacteria; (iii) depends on bacterial species, with a sensitivity order of E. coli > L. innocua > S. aureus; and (iv) increases with chitosan concentration, up to a critical point above which this effect decreases. The latter may be due to remaining proteins in chitosan acting as nutrients for bacteria therefore limiting its antibacterial activity. These results on the direct use of chitosan powder and flakes as potential antimicrobial agents for food protection at pH values lower than the chitosan pKa (6.2–6.7) are promising. |
topic |
chitosan antibacterial activity E. coli L. innocua S. aureus |
url |
http://www.mdpi.com/1420-3049/22/1/100 |
work_keys_str_mv |
AT nuryardila antibacterialactivityofneatchitosanpowderandflakes AT francedaigle antibacterialactivityofneatchitosanpowderandflakes AT marieclaudeheuzey antibacterialactivityofneatchitosanpowderandflakes AT abdellahajji antibacterialactivityofneatchitosanpowderandflakes |
_version_ |
1725692840645754880 |