Retrospective Analysis of Bacterial Cultures Sampled in German Chicken-Fattening Farms During the Years 2011–2012 Revealed Additional VIM-1 Carbapenemase-Producing Escherichia coli and a Serologically Rough Salmonella enterica Serovar Infantis
Carbapenems are last-resort antibiotics used in human medicine. The increased detection of carbapenem-resistant Enterobacteriaceae (CRE) is therefore worrying. In 2011 we reported the first livestock-associated VIM-1-producing Salmonella (S.) enterica serovar Infantis (R3) isolate from dust, sampled...
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doaj-b4513f41029f4b8aa43b113d112f676b2020-11-24T20:52:26ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-03-01910.3389/fmicb.2018.00538349828Retrospective Analysis of Bacterial Cultures Sampled in German Chicken-Fattening Farms During the Years 2011–2012 Revealed Additional VIM-1 Carbapenemase-Producing Escherichia coli and a Serologically Rough Salmonella enterica Serovar InfantisNicole Roschanski0Jennie Fischer1Linda Falgenhauer2Michael Pietsch3Sebastian Guenther4Lothar Kreienbrock5Trinad Chakraborty6Yvonne Pfeifer7Beatriz Guerra8Uwe H. Roesler9Institute for Animal Hygiene and Environmental Health, Freie Universitaet Berlin, Berlin, GermanyDepartment for Biological Safety, Federal Institute for Risk Assessment, Berlin, GermanyGerman Center for Infection Research, Institute of Medical Microbiology, Justus Liebig University Giessen, Partner Site Giessen-Marburg-Langen, Giessen, GermanyFG13 Nosocomial Pathogens and Antibiotic Resistance, Robert Koch Institute, Wernigerode, GermanyInstitute for Animal Hygiene and Environmental Health, Freie Universitaet Berlin, Berlin, GermanyEpidemiology and Information Processing and WHO Collaborating Center for Research and Training for Health at the Human-Animal-Environment Interface, Institute for Biometry, University of Veterinary Medicine Hannover, Hannover, GermanyGerman Center for Infection Research, Institute of Medical Microbiology, Justus Liebig University Giessen, Partner Site Giessen-Marburg-Langen, Giessen, GermanyFG13 Nosocomial Pathogens and Antibiotic Resistance, Robert Koch Institute, Wernigerode, GermanyDepartment for Biological Safety, Federal Institute for Risk Assessment, Berlin, GermanyInstitute for Animal Hygiene and Environmental Health, Freie Universitaet Berlin, Berlin, GermanyCarbapenems are last-resort antibiotics used in human medicine. The increased detection of carbapenem-resistant Enterobacteriaceae (CRE) is therefore worrying. In 2011 we reported the first livestock-associated VIM-1-producing Salmonella (S.) enterica serovar Infantis (R3) isolate from dust, sampled in a German chicken fattening farm. Due to this observation we retrospectively investigated more than 536 stored bacterial cultures, isolated from 45 chicken fattening farms during the years 2011 and 2012. After a non-selective overnight incubation, the bacteria were transferred to selective media. Escherichia (E.) coli and Salmonella growing on these media were further investigated, including antibiotic susceptibility testing, carbapenemase gene screening and whole genome sequencing (WGS). In total, four CRE were found in three out of 45 investigated farms: Besides R3, one additional Salmonella (G-336-1a) as well as two E. coli isolates (G-336-2, G-268-2). All but G-268-2 harbored the blaVIM-1 gene. Salmonella isolates R3 and G-336-1 were closely related although derived from two different farms. All three blaVIM-1-encoding isolates possessed identical plasmids and the blaVIM-1- containing transposon showed mobility at least in vitro. In isolate G-268-2, the AmpC beta-lactamase gene blaCMY-2 but no known carbapenemase gene was identified. However, a transfer of the phenotypic resistance was possible. Furthermore, G-268-2 contained the mcr-1 gene, combining phenotypical carbapenem- as well as colistin resistance in one isolate. Carbapenem-resistant Enterobacteriaceae have been found in three out of 45 investigated chicken flocks. This finding is alarming and emphasizes the importance of intervention strategies to contain the environmental spread of resistant bacteria in animals and humans.http://journal.frontiersin.org/article/10.3389/fmicb.2018.00538/fullantimicrobial resistanceplasmidslivestockcarbapenemsVIM-1 producing EnterobacteriaceaeGermany |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nicole Roschanski Jennie Fischer Linda Falgenhauer Michael Pietsch Sebastian Guenther Lothar Kreienbrock Trinad Chakraborty Yvonne Pfeifer Beatriz Guerra Uwe H. Roesler |
spellingShingle |
Nicole Roschanski Jennie Fischer Linda Falgenhauer Michael Pietsch Sebastian Guenther Lothar Kreienbrock Trinad Chakraborty Yvonne Pfeifer Beatriz Guerra Uwe H. Roesler Retrospective Analysis of Bacterial Cultures Sampled in German Chicken-Fattening Farms During the Years 2011–2012 Revealed Additional VIM-1 Carbapenemase-Producing Escherichia coli and a Serologically Rough Salmonella enterica Serovar Infantis Frontiers in Microbiology antimicrobial resistance plasmids livestock carbapenems VIM-1 producing Enterobacteriaceae Germany |
author_facet |
Nicole Roschanski Jennie Fischer Linda Falgenhauer Michael Pietsch Sebastian Guenther Lothar Kreienbrock Trinad Chakraborty Yvonne Pfeifer Beatriz Guerra Uwe H. Roesler |
author_sort |
Nicole Roschanski |
title |
Retrospective Analysis of Bacterial Cultures Sampled in German Chicken-Fattening Farms During the Years 2011–2012 Revealed Additional VIM-1 Carbapenemase-Producing Escherichia coli and a Serologically Rough Salmonella enterica Serovar Infantis |
title_short |
Retrospective Analysis of Bacterial Cultures Sampled in German Chicken-Fattening Farms During the Years 2011–2012 Revealed Additional VIM-1 Carbapenemase-Producing Escherichia coli and a Serologically Rough Salmonella enterica Serovar Infantis |
title_full |
Retrospective Analysis of Bacterial Cultures Sampled in German Chicken-Fattening Farms During the Years 2011–2012 Revealed Additional VIM-1 Carbapenemase-Producing Escherichia coli and a Serologically Rough Salmonella enterica Serovar Infantis |
title_fullStr |
Retrospective Analysis of Bacterial Cultures Sampled in German Chicken-Fattening Farms During the Years 2011–2012 Revealed Additional VIM-1 Carbapenemase-Producing Escherichia coli and a Serologically Rough Salmonella enterica Serovar Infantis |
title_full_unstemmed |
Retrospective Analysis of Bacterial Cultures Sampled in German Chicken-Fattening Farms During the Years 2011–2012 Revealed Additional VIM-1 Carbapenemase-Producing Escherichia coli and a Serologically Rough Salmonella enterica Serovar Infantis |
title_sort |
retrospective analysis of bacterial cultures sampled in german chicken-fattening farms during the years 2011–2012 revealed additional vim-1 carbapenemase-producing escherichia coli and a serologically rough salmonella enterica serovar infantis |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2018-03-01 |
description |
Carbapenems are last-resort antibiotics used in human medicine. The increased detection of carbapenem-resistant Enterobacteriaceae (CRE) is therefore worrying. In 2011 we reported the first livestock-associated VIM-1-producing Salmonella (S.) enterica serovar Infantis (R3) isolate from dust, sampled in a German chicken fattening farm. Due to this observation we retrospectively investigated more than 536 stored bacterial cultures, isolated from 45 chicken fattening farms during the years 2011 and 2012. After a non-selective overnight incubation, the bacteria were transferred to selective media. Escherichia (E.) coli and Salmonella growing on these media were further investigated, including antibiotic susceptibility testing, carbapenemase gene screening and whole genome sequencing (WGS). In total, four CRE were found in three out of 45 investigated farms: Besides R3, one additional Salmonella (G-336-1a) as well as two E. coli isolates (G-336-2, G-268-2). All but G-268-2 harbored the blaVIM-1 gene. Salmonella isolates R3 and G-336-1 were closely related although derived from two different farms. All three blaVIM-1-encoding isolates possessed identical plasmids and the blaVIM-1- containing transposon showed mobility at least in vitro. In isolate G-268-2, the AmpC beta-lactamase gene blaCMY-2 but no known carbapenemase gene was identified. However, a transfer of the phenotypic resistance was possible. Furthermore, G-268-2 contained the mcr-1 gene, combining phenotypical carbapenem- as well as colistin resistance in one isolate. Carbapenem-resistant Enterobacteriaceae have been found in three out of 45 investigated chicken flocks. This finding is alarming and emphasizes the importance of intervention strategies to contain the environmental spread of resistant bacteria in animals and humans. |
topic |
antimicrobial resistance plasmids livestock carbapenems VIM-1 producing Enterobacteriaceae Germany |
url |
http://journal.frontiersin.org/article/10.3389/fmicb.2018.00538/full |
work_keys_str_mv |
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