Transmission of Porcine Endogenous Retrovirus Produced from Different Recipient Cells In Vivo.
Humanized pigs have been developed to reduce the incidence of immune rejection in xenotransplantation, but significant concerns remain, such as transmission of viral zoonosis. Porcine endogenous retroviruses (PERV), which exist in the genome of pigs, are produced as infectious virions from all porci...
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doaj-b48e52e20be840789a2df0f882328aee2020-11-25T00:08:36ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-011111e016515610.1371/journal.pone.0165156Transmission of Porcine Endogenous Retrovirus Produced from Different Recipient Cells In Vivo.Nayoung KimJiwon ChoiSehyun KimYong-Dae GwonYeondong ChoJae Myung YangYu-Kyoung OhYoung Bong KimHumanized pigs have been developed to reduce the incidence of immune rejection in xenotransplantation, but significant concerns remain, such as transmission of viral zoonosis. Porcine endogenous retroviruses (PERV), which exist in the genome of pigs, are produced as infectious virions from all porcine cells and cause zoonosis. Here, we examined the possibility of zoonosis of hosts under conditions of immune suppression or xenotransplantation of cells producing host-adapted viruses. Upon transplantation of PERV-producing porcine cells into mice, no transmission of PERV was detected, whereas, transmission of PERV from mice transplanted with mouse-adapted PERV-producing cells was detected. In addition, the frequency of PERV transmission was increased in CsA treated mice transplanted with PERV-producing murine cells, compared with PERV-producing porcine cells. Transmission of PERV to host animals did not affect weight but immune responses, in particular, the number of T cells from PERV-transmitted mice, were notably reduced. The observed risk of PERV zoonosis highlights the requirement for thorough evaluation of viral zoonosis under particular host conditions, such as immunosuppressive treatment and transplantation with host-adapted virus-producing cells.http://europepmc.org/articles/PMC5104465?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nayoung Kim Jiwon Choi Sehyun Kim Yong-Dae Gwon Yeondong Cho Jae Myung Yang Yu-Kyoung Oh Young Bong Kim |
spellingShingle |
Nayoung Kim Jiwon Choi Sehyun Kim Yong-Dae Gwon Yeondong Cho Jae Myung Yang Yu-Kyoung Oh Young Bong Kim Transmission of Porcine Endogenous Retrovirus Produced from Different Recipient Cells In Vivo. PLoS ONE |
author_facet |
Nayoung Kim Jiwon Choi Sehyun Kim Yong-Dae Gwon Yeondong Cho Jae Myung Yang Yu-Kyoung Oh Young Bong Kim |
author_sort |
Nayoung Kim |
title |
Transmission of Porcine Endogenous Retrovirus Produced from Different Recipient Cells In Vivo. |
title_short |
Transmission of Porcine Endogenous Retrovirus Produced from Different Recipient Cells In Vivo. |
title_full |
Transmission of Porcine Endogenous Retrovirus Produced from Different Recipient Cells In Vivo. |
title_fullStr |
Transmission of Porcine Endogenous Retrovirus Produced from Different Recipient Cells In Vivo. |
title_full_unstemmed |
Transmission of Porcine Endogenous Retrovirus Produced from Different Recipient Cells In Vivo. |
title_sort |
transmission of porcine endogenous retrovirus produced from different recipient cells in vivo. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2016-01-01 |
description |
Humanized pigs have been developed to reduce the incidence of immune rejection in xenotransplantation, but significant concerns remain, such as transmission of viral zoonosis. Porcine endogenous retroviruses (PERV), which exist in the genome of pigs, are produced as infectious virions from all porcine cells and cause zoonosis. Here, we examined the possibility of zoonosis of hosts under conditions of immune suppression or xenotransplantation of cells producing host-adapted viruses. Upon transplantation of PERV-producing porcine cells into mice, no transmission of PERV was detected, whereas, transmission of PERV from mice transplanted with mouse-adapted PERV-producing cells was detected. In addition, the frequency of PERV transmission was increased in CsA treated mice transplanted with PERV-producing murine cells, compared with PERV-producing porcine cells. Transmission of PERV to host animals did not affect weight but immune responses, in particular, the number of T cells from PERV-transmitted mice, were notably reduced. The observed risk of PERV zoonosis highlights the requirement for thorough evaluation of viral zoonosis under particular host conditions, such as immunosuppressive treatment and transplantation with host-adapted virus-producing cells. |
url |
http://europepmc.org/articles/PMC5104465?pdf=render |
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