Calcium/calmodulin-dependent kinase IV contributes to translation-dependent early synaptic potentiation in the anterior cingulate cortex of adult mice
<p>Abstract</p> <p>Calcium/calmodulin-dependent kinase IV (CaMKIV) phosphorylates the major transcription factor, cyclic AMP-responsive element binding protein (CREB), which plays key roles in synaptic plasticity and memory consolidation. Our previous study showed that long-term po...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2010-09-01
|
Series: | Molecular Brain |
Online Access: | http://www.molecularbrain.com/content/3/1/27 |
id |
doaj-b4918cff73a14def89a8c3d688e4d40b |
---|---|
record_format |
Article |
spelling |
doaj-b4918cff73a14def89a8c3d688e4d40b2020-11-24T21:40:08ZengBMCMolecular Brain1756-66062010-09-01312710.1186/1756-6606-3-27Calcium/calmodulin-dependent kinase IV contributes to translation-dependent early synaptic potentiation in the anterior cingulate cortex of adult miceToyoda HirokiZhao Ming-GaoMercaldo ValentinaChen TaoDescalzi GianninaKida SatoshiZhuo Min<p>Abstract</p> <p>Calcium/calmodulin-dependent kinase IV (CaMKIV) phosphorylates the major transcription factor, cyclic AMP-responsive element binding protein (CREB), which plays key roles in synaptic plasticity and memory consolidation. Our previous study showed that long-term potentiation (LTP) in the anterior cingulate cortex (ACC) was significantly enhanced in transgenic mice overexpressing CaMKIV. Considering that the CaMKIV-CREB pathway plays a central role in the protein synthesis-dependent LTP, it is possible that upregulation of CaMKIV contributes to enhancement of LTP by promoting protein synthesis. To test this possibility, we examined the effects of transcription and translation inhibitors on synaptic potentiation induced by pairing of synaptic activity with postsynaptic depolarization (paired training) in ACC pyramidal neurons of wild-type and CaMKIV transgenic mice. We found that synaptic potentiation induced by paired training was partially inhibited by transcription or translation inhibitors both in wild-type and CaMKIV transgenic mice; the extent of inhibition was markedly larger in the CaMKIV transgenic mice than in the wild-type mice. Biochemical and immunohistochemical studies revealed that CaMKIV was distributed in the membrane, cytosol and nucleus of ACC neurons. Our results reveal in the first time a transcription- and translation-dependent component of early synaptic LTP in adult ACC synapses, and demonstrate that CaMKIV enhances early synaptic potentiation by activating new protein synthesis.</p> http://www.molecularbrain.com/content/3/1/27 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Toyoda Hiroki Zhao Ming-Gao Mercaldo Valentina Chen Tao Descalzi Giannina Kida Satoshi Zhuo Min |
spellingShingle |
Toyoda Hiroki Zhao Ming-Gao Mercaldo Valentina Chen Tao Descalzi Giannina Kida Satoshi Zhuo Min Calcium/calmodulin-dependent kinase IV contributes to translation-dependent early synaptic potentiation in the anterior cingulate cortex of adult mice Molecular Brain |
author_facet |
Toyoda Hiroki Zhao Ming-Gao Mercaldo Valentina Chen Tao Descalzi Giannina Kida Satoshi Zhuo Min |
author_sort |
Toyoda Hiroki |
title |
Calcium/calmodulin-dependent kinase IV contributes to translation-dependent early synaptic potentiation in the anterior cingulate cortex of adult mice |
title_short |
Calcium/calmodulin-dependent kinase IV contributes to translation-dependent early synaptic potentiation in the anterior cingulate cortex of adult mice |
title_full |
Calcium/calmodulin-dependent kinase IV contributes to translation-dependent early synaptic potentiation in the anterior cingulate cortex of adult mice |
title_fullStr |
Calcium/calmodulin-dependent kinase IV contributes to translation-dependent early synaptic potentiation in the anterior cingulate cortex of adult mice |
title_full_unstemmed |
Calcium/calmodulin-dependent kinase IV contributes to translation-dependent early synaptic potentiation in the anterior cingulate cortex of adult mice |
title_sort |
calcium/calmodulin-dependent kinase iv contributes to translation-dependent early synaptic potentiation in the anterior cingulate cortex of adult mice |
publisher |
BMC |
series |
Molecular Brain |
issn |
1756-6606 |
publishDate |
2010-09-01 |
description |
<p>Abstract</p> <p>Calcium/calmodulin-dependent kinase IV (CaMKIV) phosphorylates the major transcription factor, cyclic AMP-responsive element binding protein (CREB), which plays key roles in synaptic plasticity and memory consolidation. Our previous study showed that long-term potentiation (LTP) in the anterior cingulate cortex (ACC) was significantly enhanced in transgenic mice overexpressing CaMKIV. Considering that the CaMKIV-CREB pathway plays a central role in the protein synthesis-dependent LTP, it is possible that upregulation of CaMKIV contributes to enhancement of LTP by promoting protein synthesis. To test this possibility, we examined the effects of transcription and translation inhibitors on synaptic potentiation induced by pairing of synaptic activity with postsynaptic depolarization (paired training) in ACC pyramidal neurons of wild-type and CaMKIV transgenic mice. We found that synaptic potentiation induced by paired training was partially inhibited by transcription or translation inhibitors both in wild-type and CaMKIV transgenic mice; the extent of inhibition was markedly larger in the CaMKIV transgenic mice than in the wild-type mice. Biochemical and immunohistochemical studies revealed that CaMKIV was distributed in the membrane, cytosol and nucleus of ACC neurons. Our results reveal in the first time a transcription- and translation-dependent component of early synaptic LTP in adult ACC synapses, and demonstrate that CaMKIV enhances early synaptic potentiation by activating new protein synthesis.</p> |
url |
http://www.molecularbrain.com/content/3/1/27 |
work_keys_str_mv |
AT toyodahiroki calciumcalmodulindependentkinaseivcontributestotranslationdependentearlysynapticpotentiationintheanteriorcingulatecortexofadultmice AT zhaominggao calciumcalmodulindependentkinaseivcontributestotranslationdependentearlysynapticpotentiationintheanteriorcingulatecortexofadultmice AT mercaldovalentina calciumcalmodulindependentkinaseivcontributestotranslationdependentearlysynapticpotentiationintheanteriorcingulatecortexofadultmice AT chentao calciumcalmodulindependentkinaseivcontributestotranslationdependentearlysynapticpotentiationintheanteriorcingulatecortexofadultmice AT descalzigiannina calciumcalmodulindependentkinaseivcontributestotranslationdependentearlysynapticpotentiationintheanteriorcingulatecortexofadultmice AT kidasatoshi calciumcalmodulindependentkinaseivcontributestotranslationdependentearlysynapticpotentiationintheanteriorcingulatecortexofadultmice AT zhuomin calciumcalmodulindependentkinaseivcontributestotranslationdependentearlysynapticpotentiationintheanteriorcingulatecortexofadultmice |
_version_ |
1725927961325993984 |