METTL3 Intensifies the Progress of Oral Squamous Cell Carcinoma via Modulating the m6A Amount of PRMT5 and PD-L1
N6-Methyladenosine (m6A) modification is one of the commonest chemical modifications in eukaryotic mRNAs, which has essential effects on mRNA translation, splicing, and stability. Currently, there is a rising concern on the regulatory role of m6A in tumorigenesis. As a known component in the m6A met...
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doaj-b496b028ebbc4ac598c553d7097f1d592021-09-06T00:01:05ZengHindawi LimitedJournal of Immunology Research2314-71562021-01-01202110.1155/2021/6149558METTL3 Intensifies the Progress of Oral Squamous Cell Carcinoma via Modulating the m6A Amount of PRMT5 and PD-L1Yilong Ai0Shiwei Liu1Hailing Luo2Siyuan Wu3Haigang Wei4Zhe Tang5Xia Li6Xiaozhi Lv7Chen Zou8Foshan Stomatological HospitalDepartment of StomatologyFoshan Stomatological HospitalFoshan Stomatological HospitalFoshan Stomatological HospitalFoshan Stomatological HospitalFoshan Stomatological HospitalDepartment of Oral & Maxillofacial SurgeryFoshan Stomatological HospitalN6-Methyladenosine (m6A) modification is one of the commonest chemical modifications in eukaryotic mRNAs, which has essential effects on mRNA translation, splicing, and stability. Currently, there is a rising concern on the regulatory role of m6A in tumorigenesis. As a known component in the m6A methyltransferase complex, METTL3 (methyltransferase-like 3) plays an essential role in m6A methylation. Till now, the functions of METTL3 in oral squamous cell carcinoma (OSCC) and its relative mechanism remain to be explored. In this research, through the GEPIA database, we found that high METTL3 expression has a correlation with poor prognosis of squamous cell carcinoma of head and neck. qRT-PCR displayed that METTL3 was highly expressed in OSCC cells. Functionally, METTL3 knockdown reduced the invasion, migration, and proliferation competence of OSCC cells and attenuated the activation of CD8+ T cells. In contrast, METTL3 overexpression resulted in opposite results. GEPIA, UALCAN, and SRAMP databases, PCR, western blot, and m6A RNA methylation assay confirmed the m6A modification of PRMT5 and PD-L1 mediated by METTL3. In conclusion, our results displayed that METTL3 intensified the metastasis and proliferation of OSCC by modulating the m6A amounts of PRMT5 and PD-L1, suggesting that METTL3 may be a therapeutic target for OSCC patients.http://dx.doi.org/10.1155/2021/6149558 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yilong Ai Shiwei Liu Hailing Luo Siyuan Wu Haigang Wei Zhe Tang Xia Li Xiaozhi Lv Chen Zou |
spellingShingle |
Yilong Ai Shiwei Liu Hailing Luo Siyuan Wu Haigang Wei Zhe Tang Xia Li Xiaozhi Lv Chen Zou METTL3 Intensifies the Progress of Oral Squamous Cell Carcinoma via Modulating the m6A Amount of PRMT5 and PD-L1 Journal of Immunology Research |
author_facet |
Yilong Ai Shiwei Liu Hailing Luo Siyuan Wu Haigang Wei Zhe Tang Xia Li Xiaozhi Lv Chen Zou |
author_sort |
Yilong Ai |
title |
METTL3 Intensifies the Progress of Oral Squamous Cell Carcinoma via Modulating the m6A Amount of PRMT5 and PD-L1 |
title_short |
METTL3 Intensifies the Progress of Oral Squamous Cell Carcinoma via Modulating the m6A Amount of PRMT5 and PD-L1 |
title_full |
METTL3 Intensifies the Progress of Oral Squamous Cell Carcinoma via Modulating the m6A Amount of PRMT5 and PD-L1 |
title_fullStr |
METTL3 Intensifies the Progress of Oral Squamous Cell Carcinoma via Modulating the m6A Amount of PRMT5 and PD-L1 |
title_full_unstemmed |
METTL3 Intensifies the Progress of Oral Squamous Cell Carcinoma via Modulating the m6A Amount of PRMT5 and PD-L1 |
title_sort |
mettl3 intensifies the progress of oral squamous cell carcinoma via modulating the m6a amount of prmt5 and pd-l1 |
publisher |
Hindawi Limited |
series |
Journal of Immunology Research |
issn |
2314-7156 |
publishDate |
2021-01-01 |
description |
N6-Methyladenosine (m6A) modification is one of the commonest chemical modifications in eukaryotic mRNAs, which has essential effects on mRNA translation, splicing, and stability. Currently, there is a rising concern on the regulatory role of m6A in tumorigenesis. As a known component in the m6A methyltransferase complex, METTL3 (methyltransferase-like 3) plays an essential role in m6A methylation. Till now, the functions of METTL3 in oral squamous cell carcinoma (OSCC) and its relative mechanism remain to be explored. In this research, through the GEPIA database, we found that high METTL3 expression has a correlation with poor prognosis of squamous cell carcinoma of head and neck. qRT-PCR displayed that METTL3 was highly expressed in OSCC cells. Functionally, METTL3 knockdown reduced the invasion, migration, and proliferation competence of OSCC cells and attenuated the activation of CD8+ T cells. In contrast, METTL3 overexpression resulted in opposite results. GEPIA, UALCAN, and SRAMP databases, PCR, western blot, and m6A RNA methylation assay confirmed the m6A modification of PRMT5 and PD-L1 mediated by METTL3. In conclusion, our results displayed that METTL3 intensified the metastasis and proliferation of OSCC by modulating the m6A amounts of PRMT5 and PD-L1, suggesting that METTL3 may be a therapeutic target for OSCC patients. |
url |
http://dx.doi.org/10.1155/2021/6149558 |
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