Characterization of antibiotic resistance phenotypes and resistance genes in Enterococcus spp. isolated from cheeses
Strains of Enterococcus spp. isolated from a collection of 123 artisanal and industrial cheese samples were studied for the phenotypic and genotypic assessment of antibiotic resistance. A total of 226 isolates included 119 E. faecium (52.65%), 40 E. durans (17.7%), 37 E. hirae (16.37%), 29...
Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
Published: |
University of Belgrade, University of Novi Sad
2015-01-01
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Series: | Archives of Biological Sciences |
Subjects: | |
Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2015/0354-46641400016B.pdf |
Summary: | Strains of Enterococcus spp. isolated from a collection of 123 artisanal and
industrial cheese samples were studied for the phenotypic and genotypic
assessment of antibiotic resistance. A total of 226 isolates included 119 E.
faecium (52.65%), 40 E. durans (17.7%), 37 E. hirae (16.37%), 29 E. faecalis
(12.83%) and 1 E. gallinarum (0.44%). Out of 61 tested strains, 15 (24.59%)
strains exhibited resistance to one or more tested antibiotics, as determined
by the disc diffusion method. The resistance phenotypes were as follows:
gentamicin (45.45%), tetracycline (31.82%), erythromycin (9.09%), vancomycin
(9.09%) and penicillin (4.55%). The presence of tetracycline and erythromycin
resistance genes [tet(M), tet(L) and erm(B), respectively] and integrase gene
(int), associated with Tn916-1545 transposon family, was detected by PCR
procedures. The tet(M) gene was determined in all 7 tested strains, but none
of the analyzed strains harbored tet(L) determinant. The erm(B) gene was not
detected in 9 strains characterized by phenotypic resistance to erythromycin.
All 16 strains were positive for the presence of the int gene. The presented
results show the presence of antibiotic resistance genes and the transposon
integrase gene associated with transferable resistance in enterococci,
indicating a potential for gene transfer through the food chain. |
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ISSN: | 0354-4664 1821-4339 |