Isolation and Identification of Fourteen Microsatellite Markers in <em>Clivia miniata</em> and <em>Clivia </em><em>n</em><em>obilis</em> (Amaryllidaceae)
<em>Clivia</em> is a genus of great horticultural importance and has been widely cultivated as ornamental plants in all over the world. In order to assess the phylogenetic relationships and genetic diversity of the wild <em>Clivia</em> species...
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doaj-b4a19cd909144e8a9c8ab66774c4709f2020-11-25T00:24:55ZengMDPI AGInternational Journal of Molecular Sciences1422-00672012-08-011389609961410.3390/ijms13089609Isolation and Identification of Fourteen Microsatellite Markers in <em>Clivia miniata</em> and <em>Clivia </em><em>n</em><em>obilis</em> (Amaryllidaceae)Yue-Ying RenHong-Xing XiaoEliyas NurmamatLin-Feng LiMing-Rui LiHe ZhaoYing LiuWei Gao<em>Clivia</em> is a genus of great horticultural importance and has been widely cultivated as ornamental plants in all over the world. In order to assess the phylogenetic relationships and genetic diversity of the wild <em>Clivia</em> species and cultivars, we isolated AC-enriched repeats using FIASCO from a single clone each of <em>C</em>. <em>miniata</em> Regel. and <em>Clivia</em> <em>nobilis</em> Lindl. Of the fourteen repeats, 10 were polymorphic and 4 were monomorphic. The polymorphic marker loci were characterized using 61 <em>Clivia</em> accessions. The number of alleles ranged from two to six, observed heterozygosity ranged from 0.04 to 1.00 and expected heterozygosity ranged from 0.04 to 0.83. These microsatellite marker loci provide tools for future studies of <em>Clivia</em> species and cultivars.http://www.mdpi.com/1422-0067/13/8/9609<em>Clivia miniata</em><em>Clivia nobilis</em>genetics diversitymicrosatellite |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yue-Ying Ren Hong-Xing Xiao Eliyas Nurmamat Lin-Feng Li Ming-Rui Li He Zhao Ying Liu Wei Gao |
spellingShingle |
Yue-Ying Ren Hong-Xing Xiao Eliyas Nurmamat Lin-Feng Li Ming-Rui Li He Zhao Ying Liu Wei Gao Isolation and Identification of Fourteen Microsatellite Markers in <em>Clivia miniata</em> and <em>Clivia </em><em>n</em><em>obilis</em> (Amaryllidaceae) International Journal of Molecular Sciences <em>Clivia miniata</em> <em>Clivia nobilis</em> genetics diversity microsatellite |
author_facet |
Yue-Ying Ren Hong-Xing Xiao Eliyas Nurmamat Lin-Feng Li Ming-Rui Li He Zhao Ying Liu Wei Gao |
author_sort |
Yue-Ying Ren |
title |
Isolation and Identification of Fourteen Microsatellite Markers in <em>Clivia miniata</em> and <em>Clivia </em><em>n</em><em>obilis</em> (Amaryllidaceae) |
title_short |
Isolation and Identification of Fourteen Microsatellite Markers in <em>Clivia miniata</em> and <em>Clivia </em><em>n</em><em>obilis</em> (Amaryllidaceae) |
title_full |
Isolation and Identification of Fourteen Microsatellite Markers in <em>Clivia miniata</em> and <em>Clivia </em><em>n</em><em>obilis</em> (Amaryllidaceae) |
title_fullStr |
Isolation and Identification of Fourteen Microsatellite Markers in <em>Clivia miniata</em> and <em>Clivia </em><em>n</em><em>obilis</em> (Amaryllidaceae) |
title_full_unstemmed |
Isolation and Identification of Fourteen Microsatellite Markers in <em>Clivia miniata</em> and <em>Clivia </em><em>n</em><em>obilis</em> (Amaryllidaceae) |
title_sort |
isolation and identification of fourteen microsatellite markers in <em>clivia miniata</em> and <em>clivia </em><em>n</em><em>obilis</em> (amaryllidaceae) |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2012-08-01 |
description |
<em>Clivia</em> is a genus of great horticultural importance and has been widely cultivated as ornamental plants in all over the world. In order to assess the phylogenetic relationships and genetic diversity of the wild <em>Clivia</em> species and cultivars, we isolated AC-enriched repeats using FIASCO from a single clone each of <em>C</em>. <em>miniata</em> Regel. and <em>Clivia</em> <em>nobilis</em> Lindl. Of the fourteen repeats, 10 were polymorphic and 4 were monomorphic. The polymorphic marker loci were characterized using 61 <em>Clivia</em> accessions. The number of alleles ranged from two to six, observed heterozygosity ranged from 0.04 to 1.00 and expected heterozygosity ranged from 0.04 to 0.83. These microsatellite marker loci provide tools for future studies of <em>Clivia</em> species and cultivars. |
topic |
<em>Clivia miniata</em> <em>Clivia nobilis</em> genetics diversity microsatellite |
url |
http://www.mdpi.com/1422-0067/13/8/9609 |
work_keys_str_mv |
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