Caffeine taste signaling in Drosophila larvae
The Drosophila larva has a simple peripheral nervous system with a comparably small number of sensory neurons located externally at the head or internally along the pharynx to assess its chemical environment. It is assumed that larval taste coding occurs mainly via external organs (the dorsal, termi...
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doaj-b4ad0b1367444f9d942d7a0d8ecb33632020-11-24T20:49:53ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022016-08-011010.3389/fncel.2016.00193215225Caffeine taste signaling in Drosophila larvaeAnthi A Apostolopoulou0Anthi A Apostolopoulou1Saskia Köhn2Bernhard Stehle3Michael Lutz4Alexander Wüst5Lorena Mazija6Anna Rist7C G Galizia8Alja Lüdke9Andreas Stephan Thum10University of KonstanzUniversity of SheffieldUniversity of KonstanzUniversity of KonstanzUniversity of KonstanzUniversity of KonstanzUniversity of KonstanzUniversity of KonstanzUniversity of KonstanzUniversity of KonstanzUniversity of KonstanzThe Drosophila larva has a simple peripheral nervous system with a comparably small number of sensory neurons located externally at the head or internally along the pharynx to assess its chemical environment. It is assumed that larval taste coding occurs mainly via external organs (the dorsal, terminal and ventral organ). However, the contribution of the internal pharyngeal sensory organs has not been explored. Here we find that larvae require a single pharyngeal gustatory receptor neuron pair called D1, which is located in the dorsal pharyngeal sensilla, in order to avoid caffeine and to associate an odor with caffeine punishment. In contrast, caffeine-driven reduction in feeding in non-choice situations does not require D1. Hence, this work provides data on taste coding via different receptor neurons, depending on the behavioral context. Furthermore, we show that the larval pharyngeal system is involved in bitter tasting. Using ectopic expressions, we show that the caffeine receptor in neuron D1 requires the function of at least four receptor genes: the putative coreceptors Gr33a, Gr66a, the putative caffeine-specific receptor Gr93a, and yet unknown additional molecular component(s). This suggests that larval taste perception is more complex than previously assumed already at the sensory level. Taste information from different sensory organs located outside at the head or inside along the pharynx of the larva is assembled to trigger taste guided behaviours.http://journal.frontiersin.org/Journal/10.3389/fncel.2016.00193/fullCaffeineFeeding Behaviorlearning and memorysingle cell analysischoice behaviourgustatory receptor (GR) |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Anthi A Apostolopoulou Anthi A Apostolopoulou Saskia Köhn Bernhard Stehle Michael Lutz Alexander Wüst Lorena Mazija Anna Rist C G Galizia Alja Lüdke Andreas Stephan Thum |
spellingShingle |
Anthi A Apostolopoulou Anthi A Apostolopoulou Saskia Köhn Bernhard Stehle Michael Lutz Alexander Wüst Lorena Mazija Anna Rist C G Galizia Alja Lüdke Andreas Stephan Thum Caffeine taste signaling in Drosophila larvae Frontiers in Cellular Neuroscience Caffeine Feeding Behavior learning and memory single cell analysis choice behaviour gustatory receptor (GR) |
author_facet |
Anthi A Apostolopoulou Anthi A Apostolopoulou Saskia Köhn Bernhard Stehle Michael Lutz Alexander Wüst Lorena Mazija Anna Rist C G Galizia Alja Lüdke Andreas Stephan Thum |
author_sort |
Anthi A Apostolopoulou |
title |
Caffeine taste signaling in Drosophila larvae |
title_short |
Caffeine taste signaling in Drosophila larvae |
title_full |
Caffeine taste signaling in Drosophila larvae |
title_fullStr |
Caffeine taste signaling in Drosophila larvae |
title_full_unstemmed |
Caffeine taste signaling in Drosophila larvae |
title_sort |
caffeine taste signaling in drosophila larvae |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cellular Neuroscience |
issn |
1662-5102 |
publishDate |
2016-08-01 |
description |
The Drosophila larva has a simple peripheral nervous system with a comparably small number of sensory neurons located externally at the head or internally along the pharynx to assess its chemical environment. It is assumed that larval taste coding occurs mainly via external organs (the dorsal, terminal and ventral organ). However, the contribution of the internal pharyngeal sensory organs has not been explored. Here we find that larvae require a single pharyngeal gustatory receptor neuron pair called D1, which is located in the dorsal pharyngeal sensilla, in order to avoid caffeine and to associate an odor with caffeine punishment. In contrast, caffeine-driven reduction in feeding in non-choice situations does not require D1. Hence, this work provides data on taste coding via different receptor neurons, depending on the behavioral context. Furthermore, we show that the larval pharyngeal system is involved in bitter tasting. Using ectopic expressions, we show that the caffeine receptor in neuron D1 requires the function of at least four receptor genes: the putative coreceptors Gr33a, Gr66a, the putative caffeine-specific receptor Gr93a, and yet unknown additional molecular component(s). This suggests that larval taste perception is more complex than previously assumed already at the sensory level. Taste information from different sensory organs located outside at the head or inside along the pharynx of the larva is assembled to trigger taste guided behaviours. |
topic |
Caffeine Feeding Behavior learning and memory single cell analysis choice behaviour gustatory receptor (GR) |
url |
http://journal.frontiersin.org/Journal/10.3389/fncel.2016.00193/full |
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