Influence of Gelatin-Thrombin Matrix Tissue Sealant on Bacterial Colony Formation and Risk of Pelvic Infection
Objective. Gelatin-thrombin matrix (GTM) tissue sealant use was previously identified as an independent predictor of pelvic infection following hysterectomies. We aim to elucidate contributing factors by assessing influence of GTM on bacterial colony formation and characterizing bacteria present at...
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doaj-b4bf56ffc8dc4cba968c27099564ff642020-11-24T20:59:01ZengHindawi LimitedInfectious Diseases in Obstetrics and Gynecology1064-74491098-09972016-01-01201610.1155/2016/26497082649708Influence of Gelatin-Thrombin Matrix Tissue Sealant on Bacterial Colony Formation and Risk of Pelvic InfectionMichael J. Jarrett0Andres Vázquez-Torres1Daniel N. Frank2Bruce D. McCollister3Patrick K. Henthorn4Diana Ir5Jeanelle Sheeder6Michael S. Guy7Hiba Q. Anwar8Kian Behbakht9Research Track, University of Colorado School of Medicine, Aurora, CO 80045, USADepartment of Immunology and Microbiology, University of Colorado School of Medicine, Anschutz Medical Campus, Aurora, CO 80045, USADivision of Infectious Diseases, Department of Medicine, University of Colorado School of Medicine, Anschutz Medical Campus, Aurora, CO 80045, USADivision of Infectious Diseases, Department of Medicine, University of Colorado School of Medicine, Anschutz Medical Campus, Aurora, CO 80045, USAResearch Track, University of Colorado School of Medicine, Aurora, CO 80045, USADivision of Infectious Diseases, Department of Medicine, University of Colorado School of Medicine, Anschutz Medical Campus, Aurora, CO 80045, USADivision of Family Planning, Department of OB/GYN, University of Colorado School of Medicine, Anschutz Medical Campus, Aurora, CO 80045, USADivision of Gynecologic Oncology, Department of OB/GYN, University of Colorado School of Medicine, Anschutz Medical Campus, Aurora, CO 80045, USADivision of Gynecologic Oncology, Department of OB/GYN, University of Colorado School of Medicine, Anschutz Medical Campus, Aurora, CO 80045, USADivision of Gynecologic Oncology, Department of OB/GYN, University of Colorado School of Medicine, Anschutz Medical Campus, Aurora, CO 80045, USAObjective. Gelatin-thrombin matrix (GTM) tissue sealant use was previously identified as an independent predictor of pelvic infection following hysterectomies. We aim to elucidate contributing factors by assessing influence of GTM on bacterial colony formation and characterizing bacteria present at the vaginal cuff. Methods. Escherichia coli was incubated in phosphate-buffered saline (PBS) and pelvic washings with and without GTM to assess influence on colony formation. Pelvic washings of the vaginal cuff were collected from hysterectomies occurring from June through October 2015. In vitro techniques, 16S rRNA gene qPCR, and 16S amplicon sequencing were performed with washings to characterize bacteria at the vaginal cuff. Results. Mean bacterial colony formation in PBS was greater for E. coli incubated in the presence of GTM (1.48 × 107 CFU/mL) versus without (9.95 × 105 CFU/mL) following 20-hour incubation (p=0.001). Out of 61 pelvic washings samples, 3 were culture positive (≥5000 CFU/mL) with Enterococcus faecalis. Conclusion. In vitro experiments support a facilitating role of GTM on colony formation of E. coli in PBS. However, given the negative results of surgical site washings following adequate disinfection, the role of GTM in promoting posthysterectomy pelvic infections may be limited. Analysis of pelvic washings revealed presence of E. faecalis, but results were inconclusive. Further studies are recommended.http://dx.doi.org/10.1155/2016/2649708 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Michael J. Jarrett Andres Vázquez-Torres Daniel N. Frank Bruce D. McCollister Patrick K. Henthorn Diana Ir Jeanelle Sheeder Michael S. Guy Hiba Q. Anwar Kian Behbakht |
spellingShingle |
Michael J. Jarrett Andres Vázquez-Torres Daniel N. Frank Bruce D. McCollister Patrick K. Henthorn Diana Ir Jeanelle Sheeder Michael S. Guy Hiba Q. Anwar Kian Behbakht Influence of Gelatin-Thrombin Matrix Tissue Sealant on Bacterial Colony Formation and Risk of Pelvic Infection Infectious Diseases in Obstetrics and Gynecology |
author_facet |
Michael J. Jarrett Andres Vázquez-Torres Daniel N. Frank Bruce D. McCollister Patrick K. Henthorn Diana Ir Jeanelle Sheeder Michael S. Guy Hiba Q. Anwar Kian Behbakht |
author_sort |
Michael J. Jarrett |
title |
Influence of Gelatin-Thrombin Matrix Tissue Sealant on Bacterial Colony Formation and Risk of Pelvic Infection |
title_short |
Influence of Gelatin-Thrombin Matrix Tissue Sealant on Bacterial Colony Formation and Risk of Pelvic Infection |
title_full |
Influence of Gelatin-Thrombin Matrix Tissue Sealant on Bacterial Colony Formation and Risk of Pelvic Infection |
title_fullStr |
Influence of Gelatin-Thrombin Matrix Tissue Sealant on Bacterial Colony Formation and Risk of Pelvic Infection |
title_full_unstemmed |
Influence of Gelatin-Thrombin Matrix Tissue Sealant on Bacterial Colony Formation and Risk of Pelvic Infection |
title_sort |
influence of gelatin-thrombin matrix tissue sealant on bacterial colony formation and risk of pelvic infection |
publisher |
Hindawi Limited |
series |
Infectious Diseases in Obstetrics and Gynecology |
issn |
1064-7449 1098-0997 |
publishDate |
2016-01-01 |
description |
Objective. Gelatin-thrombin matrix (GTM) tissue sealant use was previously identified as an independent predictor of pelvic infection following hysterectomies. We aim to elucidate contributing factors by assessing influence of GTM on bacterial colony formation and characterizing bacteria present at the vaginal cuff. Methods. Escherichia coli was incubated in phosphate-buffered saline (PBS) and pelvic washings with and without GTM to assess influence on colony formation. Pelvic washings of the vaginal cuff were collected from hysterectomies occurring from June through October 2015. In vitro techniques, 16S rRNA gene qPCR, and 16S amplicon sequencing were performed with washings to characterize bacteria at the vaginal cuff. Results. Mean bacterial colony formation in PBS was greater for E. coli incubated in the presence of GTM (1.48 × 107 CFU/mL) versus without (9.95 × 105 CFU/mL) following 20-hour incubation (p=0.001). Out of 61 pelvic washings samples, 3 were culture positive (≥5000 CFU/mL) with Enterococcus faecalis. Conclusion. In vitro experiments support a facilitating role of GTM on colony formation of E. coli in PBS. However, given the negative results of surgical site washings following adequate disinfection, the role of GTM in promoting posthysterectomy pelvic infections may be limited. Analysis of pelvic washings revealed presence of E. faecalis, but results were inconclusive. Further studies are recommended. |
url |
http://dx.doi.org/10.1155/2016/2649708 |
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