DuplicationDetector, a light weight tool for duplication detection using NGS data
Duplications are one on the main evolutionary forces in angiosperm, especially in Poaceae. A large number of genes involved in various metabolisms and pathways originate from such duplications (whole genome, segmental or single gene). However, to detect such duplication may be complicated, costly an...
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doaj-b4caf2cb18264561b993f24544bd44a72020-11-24T21:29:46ZengElsevierCurrent Plant Biology2214-66282017-06-019C232810.1016/j.cpb.2017.07.001DuplicationDetector, a light weight tool for duplication detection using NGS dataGustave Djedatin0Cécile Monat1Stefan Engelen2Francois Sabot3BIOGENOM Laboratory, FAST/DASSA, BP 14 Dassa-Zoumé, BeninDIADE UMR IRD/UM–Centre IRD de Montpellier, 911 av Agropolis BP 604501, F-34 394 Montpellier Cedex 5, FranceCommissariat à l’Energie Atomique (CEA), Institut de Génomique (IG), Genoscope, BP5706, F-91057 Evry, FranceDIADE UMR IRD/UM–Centre IRD de Montpellier, 911 av Agropolis BP 604501, F-34 394 Montpellier Cedex 5, FranceDuplications are one on the main evolutionary forces in angiosperm, especially in Poaceae. A large number of genes involved in various metabolisms and pathways originate from such duplications (whole genome, segmental or single gene). However, to detect such duplication may be complicated, costly and generally requires heavy human and material investments. Here, we propose an alternative approach for detecting putative recent segmental duplications in haploid or diploid homozygous organisms based on NGS data. We rely on abusive mappings of paralogous sequences that increase apparent heterozygous points at a given locus to identify such duplicated genomic regions. We test our tool on simulated data, then on true rice genomic sequences and were able to identify about 200 candidate duplicated genes in African rice (Oryza glaberrima) lineage compared to Asian one (O. sativa).http://www.sciencedirect.com/science/article/pii/S2214662817300476DuplicationNGSRice |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gustave Djedatin Cécile Monat Stefan Engelen Francois Sabot |
spellingShingle |
Gustave Djedatin Cécile Monat Stefan Engelen Francois Sabot DuplicationDetector, a light weight tool for duplication detection using NGS data Current Plant Biology Duplication NGS Rice |
author_facet |
Gustave Djedatin Cécile Monat Stefan Engelen Francois Sabot |
author_sort |
Gustave Djedatin |
title |
DuplicationDetector, a light weight tool for duplication detection using NGS data |
title_short |
DuplicationDetector, a light weight tool for duplication detection using NGS data |
title_full |
DuplicationDetector, a light weight tool for duplication detection using NGS data |
title_fullStr |
DuplicationDetector, a light weight tool for duplication detection using NGS data |
title_full_unstemmed |
DuplicationDetector, a light weight tool for duplication detection using NGS data |
title_sort |
duplicationdetector, a light weight tool for duplication detection using ngs data |
publisher |
Elsevier |
series |
Current Plant Biology |
issn |
2214-6628 |
publishDate |
2017-06-01 |
description |
Duplications are one on the main evolutionary forces in angiosperm, especially in Poaceae. A large number of genes involved in various metabolisms and pathways originate from such duplications (whole genome, segmental or single gene). However, to detect such duplication may be complicated, costly and generally requires heavy human and material investments. Here, we propose an alternative approach for detecting putative recent segmental duplications in haploid or diploid homozygous organisms based on NGS data. We rely on abusive mappings of paralogous sequences that increase apparent heterozygous points at a given locus to identify such duplicated genomic regions. We test our tool on simulated data, then on true rice genomic sequences and were able to identify about 200 candidate duplicated genes in African rice (Oryza glaberrima) lineage compared to Asian one (O. sativa). |
topic |
Duplication NGS Rice |
url |
http://www.sciencedirect.com/science/article/pii/S2214662817300476 |
work_keys_str_mv |
AT gustavedjedatin duplicationdetectoralightweighttoolforduplicationdetectionusingngsdata AT cecilemonat duplicationdetectoralightweighttoolforduplicationdetectionusingngsdata AT stefanengelen duplicationdetectoralightweighttoolforduplicationdetectionusingngsdata AT francoissabot duplicationdetectoralightweighttoolforduplicationdetectionusingngsdata |
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1725965841740070912 |