Enterocytozoon bieneusi in Minks (Neovison vison) in Northern China: A Public Health Concern
Enterocytozoon bieneusi is the most important causative agent of microsporidiosis and can infect almost all vertebrate and invertebrate hosts, including minks (Neovison vison). In the present study, a total of 298 feces samples (including 79 from Heilongjiang province, 31 from Hebei province, 67 fro...
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doaj-b4e9e8a662c7422e98beb2d65ec419702020-11-24T21:19:25ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-06-01910.3389/fmicb.2018.01221376199Enterocytozoon bieneusi in Minks (Neovison vison) in Northern China: A Public Health ConcernXiao-Xuan Zhang0Xiao-Xuan Zhang1Ruo-Lan Jiang2Jian-Gang Ma3Chao Xu4Quan Zhao5Guangyu Hou6Guo-Hua Liu7Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, College of Veterinary Medicine, Hunan Agricultural University, Changsha, ChinaCollege of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, ChinaSchool of Basic Medical Sciences, Xiangya School of Medicine, Central South University, Changsha, ChinaCollege of Veterinary Medicine, Northwest A&F University, Yangling, ChinaKey Laboratory of Agricultural Ministry, State Key Laboratory of Special Economic Animal Molecular Biology, Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences, Changchun, ChinaCollege of Animal Science and Technology, Changchun Sci-Tech University, Changchun, ChinaCollege of Basic Medicine, Mudanjiang Medical College, Mudanjiang, ChinaHunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, College of Veterinary Medicine, Hunan Agricultural University, Changsha, ChinaEnterocytozoon bieneusi is the most important causative agent of microsporidiosis and can infect almost all vertebrate and invertebrate hosts, including minks (Neovison vison). In the present study, a total of 298 feces samples (including 79 from Heilongjiang province, 31 from Hebei province, 67 from Jilin province, 90 from Liaoning province, and 31 from Shandong province, Northern China) were examined by nested PCR amplification of the internal transcribed spacer (ITS) region of the rRNA gene. The overall prevalence of E. bieneusi in minks was 10.1%, with 10.5% in Jilin province, 32.3% in Hebei province, 8.9% in Liaoning province, 0% in Shandong province, and 6.3% in Heilongjiang province. Furthermore, multiple logistic regression analysis revealed that region was only risk factors associated with E. bieneusi infection in the investigated minks. Five E. bieneusi ITS genotypes (three known genotypes, namely D, Peru11, and EbpC; two novel genotypes, namely, NCM-1 and NCM-2) were found in the current study. Importantly, genotypes D, Peru11 and EbpC, previously identified in humans, were also found in minks, which suggested that minks are the potential sources of human microsporidiosis. To the best of our knowledge, this is the first report of E. bieneusi infection in minks worldwide. The results of the present survey have implications for the controlling E. bieneusi infection in minks, other animals and humans.https://www.frontiersin.org/article/10.3389/fmicb.2018.01221/fullEnterocytozoon bieneusiprevalenceminksgenotypingNorthern China |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiao-Xuan Zhang Xiao-Xuan Zhang Ruo-Lan Jiang Jian-Gang Ma Chao Xu Quan Zhao Guangyu Hou Guo-Hua Liu |
spellingShingle |
Xiao-Xuan Zhang Xiao-Xuan Zhang Ruo-Lan Jiang Jian-Gang Ma Chao Xu Quan Zhao Guangyu Hou Guo-Hua Liu Enterocytozoon bieneusi in Minks (Neovison vison) in Northern China: A Public Health Concern Frontiers in Microbiology Enterocytozoon bieneusi prevalence minks genotyping Northern China |
author_facet |
Xiao-Xuan Zhang Xiao-Xuan Zhang Ruo-Lan Jiang Jian-Gang Ma Chao Xu Quan Zhao Guangyu Hou Guo-Hua Liu |
author_sort |
Xiao-Xuan Zhang |
title |
Enterocytozoon bieneusi in Minks (Neovison vison) in Northern China: A Public Health Concern |
title_short |
Enterocytozoon bieneusi in Minks (Neovison vison) in Northern China: A Public Health Concern |
title_full |
Enterocytozoon bieneusi in Minks (Neovison vison) in Northern China: A Public Health Concern |
title_fullStr |
Enterocytozoon bieneusi in Minks (Neovison vison) in Northern China: A Public Health Concern |
title_full_unstemmed |
Enterocytozoon bieneusi in Minks (Neovison vison) in Northern China: A Public Health Concern |
title_sort |
enterocytozoon bieneusi in minks (neovison vison) in northern china: a public health concern |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2018-06-01 |
description |
Enterocytozoon bieneusi is the most important causative agent of microsporidiosis and can infect almost all vertebrate and invertebrate hosts, including minks (Neovison vison). In the present study, a total of 298 feces samples (including 79 from Heilongjiang province, 31 from Hebei province, 67 from Jilin province, 90 from Liaoning province, and 31 from Shandong province, Northern China) were examined by nested PCR amplification of the internal transcribed spacer (ITS) region of the rRNA gene. The overall prevalence of E. bieneusi in minks was 10.1%, with 10.5% in Jilin province, 32.3% in Hebei province, 8.9% in Liaoning province, 0% in Shandong province, and 6.3% in Heilongjiang province. Furthermore, multiple logistic regression analysis revealed that region was only risk factors associated with E. bieneusi infection in the investigated minks. Five E. bieneusi ITS genotypes (three known genotypes, namely D, Peru11, and EbpC; two novel genotypes, namely, NCM-1 and NCM-2) were found in the current study. Importantly, genotypes D, Peru11 and EbpC, previously identified in humans, were also found in minks, which suggested that minks are the potential sources of human microsporidiosis. To the best of our knowledge, this is the first report of E. bieneusi infection in minks worldwide. The results of the present survey have implications for the controlling E. bieneusi infection in minks, other animals and humans. |
topic |
Enterocytozoon bieneusi prevalence minks genotyping Northern China |
url |
https://www.frontiersin.org/article/10.3389/fmicb.2018.01221/full |
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