Pattern of antibiotic resistant mastitis in dairy cows
Aim: To study the prevalence of drug resistant mastitis and their pattern of antibiotic resistance in dairy cows from Tamil Nadu. Materials and Methods: Isolation and identification of resistant pathogens were performed from acute clinical mastitis samples. Based on culture, isolation and sensitivi...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Veterinary World
2014-06-01
|
Series: | Veterinary World |
Subjects: | |
Online Access: | http://www.veterinaryworld.org/Vol.7/June-2014/5.pdf |
id |
doaj-b4f699a820234eb3b419530f229aef3d |
---|---|
record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
D. Chandrasekaran P. Venkatesan K. G. Tirumurugaan A. P. Nambi P. S. Thirunavukkarasu K. Kumanan S. Vairamuthu S. Ramesh |
spellingShingle |
D. Chandrasekaran P. Venkatesan K. G. Tirumurugaan A. P. Nambi P. S. Thirunavukkarasu K. Kumanan S. Vairamuthu S. Ramesh Pattern of antibiotic resistant mastitis in dairy cows Veterinary World bovine resistant mastitis Escherichia coli methicillin resistant Staphylococcus aureus Staphylococcus aureus |
author_facet |
D. Chandrasekaran P. Venkatesan K. G. Tirumurugaan A. P. Nambi P. S. Thirunavukkarasu K. Kumanan S. Vairamuthu S. Ramesh |
author_sort |
D. Chandrasekaran |
title |
Pattern of antibiotic resistant mastitis in dairy cows |
title_short |
Pattern of antibiotic resistant mastitis in dairy cows |
title_full |
Pattern of antibiotic resistant mastitis in dairy cows |
title_fullStr |
Pattern of antibiotic resistant mastitis in dairy cows |
title_full_unstemmed |
Pattern of antibiotic resistant mastitis in dairy cows |
title_sort |
pattern of antibiotic resistant mastitis in dairy cows |
publisher |
Veterinary World |
series |
Veterinary World |
issn |
0972-8988 2231-0916 |
publishDate |
2014-06-01 |
description |
Aim: To study the prevalence of drug resistant mastitis and their pattern of antibiotic resistance in dairy cows from Tamil Nadu.
Materials and Methods: Isolation and identification of resistant pathogens were performed from acute clinical mastitis samples. Based on culture, isolation and sensitivity tests, cows with resistant mastitis were grouped as; Group I: Escherichia coli (n=119), Group II: Staphylococcus aureus (n=104) and Group III: Methicillin-resistant Staphylococcal aureus (MRSA) (n=12). The isolates were tested using agar disc diffusion method for their antimicrobial susceptibility and modified resazurin assay microdilution technique for minimum inhibitory concentration (MIC) to 8 antimicrobial drugs. The organisms were also confirmed for their identity by performing PCR on the bacterial pellet targeting the specific genes such as 16s-23s rRNA, mecA and blaZ respectively for the resistant pathogens and also confirmed by sequencing.
Results: Antibiotic resistant mastitis was detected in 235 out of 401 cows accounting to 56.1%. The predominant resistant causative pathogen was E. coli (50.64%) followed by S. aureus (44.25%) and MRSA (5.11%). In vitro antibiotic sensitivity test and MIC breakpoints, E. coli, S. aureus and MRSA organisms showed more sensitivity to enrofloxacin, amoxicillin + sulbactam, gentamicin and ceftriaxone and had highest resistant to penicillin followed by amoxicillin, oxytetracycline and methicillin. E. coli and S. aureus isolates were found to be resistant to 1 or 2 antimicrobials, whereas most of the MRSA isolates were found to be multi-drug resistant i.e resistance to 3 or more of antimicrobials. Out of 235 milk samples, the specific target gene 16s-23s rRNA (E. coli ), 16s-23s rRNA (S. aureus) and MRSA (mecA and blaZ) could be amplified from 119, 104 and 12 isolates with a percentage positivity of 50.64 (119/235), 89.64 (104/116) and 10.34 (12/116) respectively.
Conclusion: Prevalence of antimicrobial resistance (AMR) in bovine mastitis pathogens was high. Most MRSA pathogens were multidrug resistant. E. coli and S. aureus isolates were resistant to few antimicrobials. |
topic |
bovine resistant mastitis Escherichia coli methicillin resistant Staphylococcus aureus Staphylococcus aureus |
url |
http://www.veterinaryworld.org/Vol.7/June-2014/5.pdf |
work_keys_str_mv |
AT dchandrasekaran patternofantibioticresistantmastitisindairycows AT pvenkatesan patternofantibioticresistantmastitisindairycows AT kgtirumurugaan patternofantibioticresistantmastitisindairycows AT apnambi patternofantibioticresistantmastitisindairycows AT psthirunavukkarasu patternofantibioticresistantmastitisindairycows AT kkumanan patternofantibioticresistantmastitisindairycows AT svairamuthu patternofantibioticresistantmastitisindairycows AT sramesh patternofantibioticresistantmastitisindairycows |
_version_ |
1721240220275310592 |
spelling |
doaj-b4f699a820234eb3b419530f229aef3d2021-08-02T06:23:59ZengVeterinary WorldVeterinary World0972-89882231-09162014-06-017638939410.14202/vetworld.2014.389-394Pattern of antibiotic resistant mastitis in dairy cows D. Chandrasekaran0P. Venkatesan1K. G. Tirumurugaan2A. P. Nambi3P. S. Thirunavukkarasu4K. Kumanan5S. Vairamuthu6S. Ramesh7Department of Veterinary Clinical Medicine, Ethics and Jurisprudence, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University (TANUVAS), Tamil Nadu, India; drchandrus@yahoo.comDepartment of Veterinary Clinical Medicine, Ethics and Jurisprudence, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University (TANUVAS), Tamil Nadu, India; venkatesanperiasamy@gmail.comDepartment of Veterinary Clinical Medicine, Ethics and Jurisprudence, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University (TANUVAS), Tamil Nadu, India; tirumurugaankg@tanuvas.org.inDepartment of Veterinary Clinical Medicine, Ethics and Jurisprudence, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University (TANUVAS), Tamil Nadu, India; nambi529@yahoo.comDepartment of Veterinary Clinical Medicine, Ethics and Jurisprudence, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University (TANUVAS), Tamil Nadu, India; pstthiru@yahoo.comDepartment of Veterinary Clinical Medicine, Ethics and Jurisprudence, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University (TANUVAS), Tamil Nadu, India; kumananrani@hotmail.com Department of Veterinary Clinical Medicine, Ethics and Jurisprudence, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University (TANUVAS), Tamil Nadu, India; vairamuthu@tanuvas.org.inDepartment of Veterinary Clinical Medicine, Ethics and Jurisprudence, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University (TANUVAS), Tamil Nadu, India; ramesh@tanuvas.org.inAim: To study the prevalence of drug resistant mastitis and their pattern of antibiotic resistance in dairy cows from Tamil Nadu. Materials and Methods: Isolation and identification of resistant pathogens were performed from acute clinical mastitis samples. Based on culture, isolation and sensitivity tests, cows with resistant mastitis were grouped as; Group I: Escherichia coli (n=119), Group II: Staphylococcus aureus (n=104) and Group III: Methicillin-resistant Staphylococcal aureus (MRSA) (n=12). The isolates were tested using agar disc diffusion method for their antimicrobial susceptibility and modified resazurin assay microdilution technique for minimum inhibitory concentration (MIC) to 8 antimicrobial drugs. The organisms were also confirmed for their identity by performing PCR on the bacterial pellet targeting the specific genes such as 16s-23s rRNA, mecA and blaZ respectively for the resistant pathogens and also confirmed by sequencing. Results: Antibiotic resistant mastitis was detected in 235 out of 401 cows accounting to 56.1%. The predominant resistant causative pathogen was E. coli (50.64%) followed by S. aureus (44.25%) and MRSA (5.11%). In vitro antibiotic sensitivity test and MIC breakpoints, E. coli, S. aureus and MRSA organisms showed more sensitivity to enrofloxacin, amoxicillin + sulbactam, gentamicin and ceftriaxone and had highest resistant to penicillin followed by amoxicillin, oxytetracycline and methicillin. E. coli and S. aureus isolates were found to be resistant to 1 or 2 antimicrobials, whereas most of the MRSA isolates were found to be multi-drug resistant i.e resistance to 3 or more of antimicrobials. Out of 235 milk samples, the specific target gene 16s-23s rRNA (E. coli ), 16s-23s rRNA (S. aureus) and MRSA (mecA and blaZ) could be amplified from 119, 104 and 12 isolates with a percentage positivity of 50.64 (119/235), 89.64 (104/116) and 10.34 (12/116) respectively. Conclusion: Prevalence of antimicrobial resistance (AMR) in bovine mastitis pathogens was high. Most MRSA pathogens were multidrug resistant. E. coli and S. aureus isolates were resistant to few antimicrobials.http://www.veterinaryworld.org/Vol.7/June-2014/5.pdfbovine resistant mastitisEscherichia colimethicillin resistant Staphylococcus aureusStaphylococcus aureus |