Plasmodium P36 determines host cell receptor usage during sporozoite invasion
Plasmodium sporozoites, the mosquito-transmitted forms of the malaria parasite, first infect the liver for an initial round of replication before the emergence of pathogenic blood stages. Sporozoites represent attractive targets for antimalarial preventive strategies, yet the mechanisms of parasite...
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Format: | Article |
Language: | English |
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eLife Sciences Publications Ltd
2017-05-01
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Series: | eLife |
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Online Access: | https://elifesciences.org/articles/25903 |
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doaj-b4f9109adff8452b8f21f8a38e13c330 |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Giulia Manzoni Carine Marinach Selma Topçu Sylvie Briquet Morgane Grand Matthieu Tolle Marion Gransagne Julien Lescar Chiara Andolina Jean-François Franetich Mirjam B Zeisel Thierry Huby Eric Rubinstein Georges Snounou Dominique Mazier François Nosten Thomas F Baumert Olivier Silvie |
spellingShingle |
Giulia Manzoni Carine Marinach Selma Topçu Sylvie Briquet Morgane Grand Matthieu Tolle Marion Gransagne Julien Lescar Chiara Andolina Jean-François Franetich Mirjam B Zeisel Thierry Huby Eric Rubinstein Georges Snounou Dominique Mazier François Nosten Thomas F Baumert Olivier Silvie Plasmodium P36 determines host cell receptor usage during sporozoite invasion eLife malaria hepatocyte sporozoite P. vivax P. berghei P. yoelii |
author_facet |
Giulia Manzoni Carine Marinach Selma Topçu Sylvie Briquet Morgane Grand Matthieu Tolle Marion Gransagne Julien Lescar Chiara Andolina Jean-François Franetich Mirjam B Zeisel Thierry Huby Eric Rubinstein Georges Snounou Dominique Mazier François Nosten Thomas F Baumert Olivier Silvie |
author_sort |
Giulia Manzoni |
title |
Plasmodium P36 determines host cell receptor usage during sporozoite invasion |
title_short |
Plasmodium P36 determines host cell receptor usage during sporozoite invasion |
title_full |
Plasmodium P36 determines host cell receptor usage during sporozoite invasion |
title_fullStr |
Plasmodium P36 determines host cell receptor usage during sporozoite invasion |
title_full_unstemmed |
Plasmodium P36 determines host cell receptor usage during sporozoite invasion |
title_sort |
plasmodium p36 determines host cell receptor usage during sporozoite invasion |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2017-05-01 |
description |
Plasmodium sporozoites, the mosquito-transmitted forms of the malaria parasite, first infect the liver for an initial round of replication before the emergence of pathogenic blood stages. Sporozoites represent attractive targets for antimalarial preventive strategies, yet the mechanisms of parasite entry into hepatocytes remain poorly understood. Here we show that the two main species causing malaria in humans, Plasmodium falciparum and Plasmodium vivax, rely on two distinct host cell surface proteins, CD81 and the Scavenger Receptor BI (SR-BI), respectively, to infect hepatocytes. By contrast, CD81 and SR-BI fulfil redundant functions during infection by the rodent parasite P. berghei. Genetic analysis of sporozoite factors reveals the 6-cysteine domain protein P36 as a major parasite determinant of host cell receptor usage. Our data provide molecular insights into the invasion pathways used by different malaria parasites to infect hepatocytes, and establish a functional link between a sporozoite putative ligand and host cell receptors. |
topic |
malaria hepatocyte sporozoite P. vivax P. berghei P. yoelii |
url |
https://elifesciences.org/articles/25903 |
work_keys_str_mv |
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doaj-b4f9109adff8452b8f21f8a38e13c3302021-05-05T13:28:56ZengeLife Sciences Publications LtdeLife2050-084X2017-05-01610.7554/eLife.25903Plasmodium P36 determines host cell receptor usage during sporozoite invasionGiulia Manzoni0Carine Marinach1Selma Topçu2Sylvie Briquet3Morgane Grand4Matthieu Tolle5Marion Gransagne6Julien Lescar7Chiara Andolina8Jean-François Franetich9Mirjam B Zeisel10Thierry Huby11https://orcid.org/0000-0001-6634-551XEric Rubinstein12https://orcid.org/0000-0001-7623-9665Georges Snounou13Dominique Mazier14François Nosten15https://orcid.org/0000-0002-7951-0745Thomas F Baumert16Olivier Silvie17https://orcid.org/0000-0002-0525-6940Sorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Centre d’Immunologie et des Maladies Infectieuses, U1135, ERL8255, Paris, FranceSorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Centre d’Immunologie et des Maladies Infectieuses, U1135, ERL8255, Paris, FranceSorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Centre d’Immunologie et des Maladies Infectieuses, U1135, ERL8255, Paris, FranceSorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Centre d’Immunologie et des Maladies Infectieuses, U1135, ERL8255, Paris, FranceSorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Centre d’Immunologie et des Maladies Infectieuses, U1135, ERL8255, Paris, FranceSorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Centre d’Immunologie et des Maladies Infectieuses, U1135, ERL8255, Paris, FranceSorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Centre d’Immunologie et des Maladies Infectieuses, U1135, ERL8255, Paris, FranceSorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Centre d’Immunologie et des Maladies Infectieuses, U1135, ERL8255, Paris, FranceShoklo Malaria Research Unit, Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Mae Sot, Thailand; Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, Oxford, United KingdomSorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Centre d’Immunologie et des Maladies Infectieuses, U1135, ERL8255, Paris, FranceINSERM, U1110, Institut de Recherche sur les Maladies Virales et Hépatiques, Strasbourg, France; Université de Strasbourg, Strasbourg, FranceSorbonne Universités, UPMC Univ Paris 06, INSERM, Institute of Cardiometabolism and Nutrition, UMR_S 1166, Paris, FranceINSERM, U935, Villejuif, France; Université Paris Sud, Institut André Lwoff, Villejuif, FranceSorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Centre d’Immunologie et des Maladies Infectieuses, U1135, ERL8255, Paris, FranceSorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Centre d’Immunologie et des Maladies Infectieuses, U1135, ERL8255, Paris, France; Assistance Publique Hôpitaux de Paris, Centre Hospitalo-Universitaire Pitié-Salpêtrière, Paris, FranceShoklo Malaria Research Unit, Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Mae Sot, Thailand; Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, Oxford, United KingdomINSERM, U1110, Institut de Recherche sur les Maladies Virales et Hépatiques, Strasbourg, France; Université de Strasbourg, Strasbourg, France; Institut Hospitalo-Universitaire, Pôle Hépato-digestif, Hopitaux Universitaires de Strasbourg, Strasbourg, FranceSorbonne Universités, UPMC Univ Paris 06, INSERM, CNRS, Centre d’Immunologie et des Maladies Infectieuses, U1135, ERL8255, Paris, FrancePlasmodium sporozoites, the mosquito-transmitted forms of the malaria parasite, first infect the liver for an initial round of replication before the emergence of pathogenic blood stages. Sporozoites represent attractive targets for antimalarial preventive strategies, yet the mechanisms of parasite entry into hepatocytes remain poorly understood. Here we show that the two main species causing malaria in humans, Plasmodium falciparum and Plasmodium vivax, rely on two distinct host cell surface proteins, CD81 and the Scavenger Receptor BI (SR-BI), respectively, to infect hepatocytes. By contrast, CD81 and SR-BI fulfil redundant functions during infection by the rodent parasite P. berghei. Genetic analysis of sporozoite factors reveals the 6-cysteine domain protein P36 as a major parasite determinant of host cell receptor usage. Our data provide molecular insights into the invasion pathways used by different malaria parasites to infect hepatocytes, and establish a functional link between a sporozoite putative ligand and host cell receptors.https://elifesciences.org/articles/25903malariahepatocytesporozoiteP. vivaxP. bergheiP. yoelii |