Isolation of coagulase-positive Staphylococcus aureus from meat and intestine of native ducks of Tabriz area
Coagulase-positive Staphylococcus aureus is introduced as the third major source of animal origin food borne disease. This bacterium can proliferate and produce toxin in protein and carbohydrate foods leading to food-poisoning. The aim of this study was isolation, identification and enumerating of S...
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Islamic Azad University, Tabriz Branch
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doaj-b5036da2cd2f4cb3bdcfc4199fb7f5e42020-11-24T22:53:21ZfasIslamic Azad University, Tabriz BranchBihdāsht-i Mavādd-i Ghaz̠āyī2228-76472476-69682014-02-0134 (12) زمستان5562517661Isolation of coagulase-positive Staphylococcus aureus from meat and intestine of native ducks of Tabriz areaA Javadi0S Rafei1F Shahian,2H Ghazi Hashtroudi3Department Of Food Hygiene, College of Veterinary, Tabriz Branch, Islamic Azad University, Tabriz, Iran.Veterinary Science Graduated, College of Veterinary, Tabriz Branch, Islamic Azad University, Tabriz, Iran.Veterinary Science Graduated, College of Veterinary, Tabriz Branch, Islamic Azad University, Tabriz, Iran.Veterinary Science Graduated, College of Veterinary, Tabriz Branch, Islamic Azad University, Tabriz, Iran.Coagulase-positive Staphylococcus aureus is introduced as the third major source of animal origin food borne disease. This bacterium can proliferate and produce toxin in protein and carbohydrate foods leading to food-poisoning. The aim of this study was isolation, identification and enumerating of S. aureus in meat as well as intestinal contents of native duck around Tabriz area. For this purpose, a total number of 35 native ducks randomly was purchased in the nearby villages of Tabriz. Fifty g of thigh meat together with 10 g of intestinal content of each carcass were sampled and analyzed according to the ISIRI protocols. The isolates were confirmed by PCR technique. Results showed that 17.14% of meat and fecal samples were found contaminated with S. aureus. Mean values of S. aureus load in meat and feces were estimated at 87 CFU/g and 64 CFU/g, respectively. It was assumed that contamination of duck meat with S. aureus can be occurred through non-hygienic practices during slaughter as well as contamination with intestinal contents and/or skin of the carcass.http://jfh.iaut.ac.ir/article_517661_85d5dfb8f1d2af67adb1d0bae91a616f.pdfStaphylococcus aureusDuckmeatIntestinePCR |
collection |
DOAJ |
language |
fas |
format |
Article |
sources |
DOAJ |
author |
A Javadi S Rafei F Shahian, H Ghazi Hashtroudi |
spellingShingle |
A Javadi S Rafei F Shahian, H Ghazi Hashtroudi Isolation of coagulase-positive Staphylococcus aureus from meat and intestine of native ducks of Tabriz area Bihdāsht-i Mavādd-i Ghaz̠āyī Staphylococcus aureus Duck meat Intestine PCR |
author_facet |
A Javadi S Rafei F Shahian, H Ghazi Hashtroudi |
author_sort |
A Javadi |
title |
Isolation of coagulase-positive Staphylococcus aureus from meat and intestine of native ducks of Tabriz area |
title_short |
Isolation of coagulase-positive Staphylococcus aureus from meat and intestine of native ducks of Tabriz area |
title_full |
Isolation of coagulase-positive Staphylococcus aureus from meat and intestine of native ducks of Tabriz area |
title_fullStr |
Isolation of coagulase-positive Staphylococcus aureus from meat and intestine of native ducks of Tabriz area |
title_full_unstemmed |
Isolation of coagulase-positive Staphylococcus aureus from meat and intestine of native ducks of Tabriz area |
title_sort |
isolation of coagulase-positive staphylococcus aureus from meat and intestine of native ducks of tabriz area |
publisher |
Islamic Azad University, Tabriz Branch |
series |
Bihdāsht-i Mavādd-i Ghaz̠āyī |
issn |
2228-7647 2476-6968 |
publishDate |
2014-02-01 |
description |
Coagulase-positive Staphylococcus aureus is introduced as the third major source of animal origin food borne disease. This bacterium can proliferate and produce toxin in protein and carbohydrate foods leading to food-poisoning. The aim of this study was isolation, identification and enumerating of S. aureus in meat as well as intestinal contents of native duck around Tabriz area. For this purpose, a total number of 35 native ducks randomly was purchased in the nearby villages of Tabriz. Fifty g of thigh meat together with 10 g of intestinal content of each carcass were sampled and analyzed according to the ISIRI protocols. The isolates were confirmed by PCR technique. Results showed that 17.14% of meat and fecal samples were found contaminated with S. aureus. Mean values of S. aureus load in meat and feces were estimated at 87 CFU/g and 64 CFU/g, respectively. It was assumed that contamination of duck meat with S. aureus can be occurred through non-hygienic practices during slaughter as well as contamination with intestinal contents and/or skin of the carcass. |
topic |
Staphylococcus aureus Duck meat Intestine PCR |
url |
http://jfh.iaut.ac.ir/article_517661_85d5dfb8f1d2af67adb1d0bae91a616f.pdf |
work_keys_str_mv |
AT ajavadi isolationofcoagulasepositivestaphylococcusaureusfrommeatandintestineofnativeducksoftabrizarea AT srafei isolationofcoagulasepositivestaphylococcusaureusfrommeatandintestineofnativeducksoftabrizarea AT fshahian isolationofcoagulasepositivestaphylococcusaureusfrommeatandintestineofnativeducksoftabrizarea AT hghazihashtroudi isolationofcoagulasepositivestaphylococcusaureusfrommeatandintestineofnativeducksoftabrizarea |
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