Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complex

Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complex

A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to clinical isolates of Mycobacterium tuberculosis complex to reduce the cost of using lyticase. This protocol reduces the expense of PFGE typing of Mycobacterium tuberculosis complex as it removes the use of lyti...

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Main Authors: Arash Ghodousi, S Vatani, Davood Darban-Sarokhalil, Maryam Omrani, AA Fooladi, Ad Khosaravi, Mohammad Mehdi Feizabadi
Format: Article
Language:English
Published: Tehran University of Medical Sciences 2012-03-01
Series:Iranian Journal of Microbiology
Subjects:
Mycobacterium tuberculosis
Pulsed-filed gel electrophoresis
TB-complex
Online Access:https://ijm.tums.ac.ir/index.php/ijm/article/view/713
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spelling doaj-b5058c0d308a4b11b8bd240de88c2cb42020-12-02T06:11:28ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472012-03-0141Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complexArash Ghodousi0S Vatani1Davood Darban-Sarokhalil2Maryam Omrani3AA Fooladi4Ad Khosaravi5Mohammad Mehdi Feizabadi6Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.ADept. of Microbiology, School of Medicine & Infectious and Tropical Diseases Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran..Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.Research Center of Molecular Biology, Department of Pathology, School of Medical Sciences, Baqiyatallah University of Medical Sciences, Tehran, Iran.ADept. of Microbiology, School of Medicine & Infectious and Tropical Diseases Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to clinical isolates of Mycobacterium tuberculosis complex to reduce the cost of using lyticase. This protocol reduces the expense of PFGE typing of Mycobacterium tuberculosis complex as it removes the use of lyticase during the spheroplast formation from these bacteria. https://ijm.tums.ac.ir/index.php/ijm/article/view/713Mycobacterium tuberculosisPulsed-filed gel electrophoresisTB-complex
collection DOAJ
language English
format Article
sources DOAJ
author Arash Ghodousi
S Vatani
Davood Darban-Sarokhalil
Maryam Omrani
AA Fooladi
Ad Khosaravi
Mohammad Mehdi Feizabadi
spellingShingle Arash Ghodousi
S Vatani
Davood Darban-Sarokhalil
Maryam Omrani
AA Fooladi
Ad Khosaravi
Mohammad Mehdi Feizabadi
Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complex
Iranian Journal of Microbiology
Mycobacterium tuberculosis
Pulsed-filed gel electrophoresis
TB-complex
author_facet Arash Ghodousi
S Vatani
Davood Darban-Sarokhalil
Maryam Omrani
AA Fooladi
Ad Khosaravi
Mohammad Mehdi Feizabadi
author_sort Arash Ghodousi
title Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complex
title_short Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complex
title_full Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complex
title_fullStr Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complex
title_full_unstemmed Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complex
title_sort development of a new dna extraction protocol for pfge typing of mycobacterium tuberculosis complex
publisher Tehran University of Medical Sciences
series Iranian Journal of Microbiology
issn 2008-3289
2008-4447
publishDate 2012-03-01
description A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to clinical isolates of Mycobacterium tuberculosis complex to reduce the cost of using lyticase. This protocol reduces the expense of PFGE typing of Mycobacterium tuberculosis complex as it removes the use of lyticase during the spheroplast formation from these bacteria.
topic Mycobacterium tuberculosis
Pulsed-filed gel electrophoresis
TB-complex
url https://ijm.tums.ac.ir/index.php/ijm/article/view/713
work_keys_str_mv AT arashghodousi developmentofanewdnaextractionprotocolforpfgetypingofmycobacteriumtuberculosiscomplex
AT svatani developmentofanewdnaextractionprotocolforpfgetypingofmycobacteriumtuberculosiscomplex
AT davooddarbansarokhalil developmentofanewdnaextractionprotocolforpfgetypingofmycobacteriumtuberculosiscomplex
AT maryamomrani developmentofanewdnaextractionprotocolforpfgetypingofmycobacteriumtuberculosiscomplex
AT aafooladi developmentofanewdnaextractionprotocolforpfgetypingofmycobacteriumtuberculosiscomplex
AT adkhosaravi developmentofanewdnaextractionprotocolforpfgetypingofmycobacteriumtuberculosiscomplex
AT mohammadmehdifeizabadi developmentofanewdnaextractionprotocolforpfgetypingofmycobacteriumtuberculosiscomplex
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