Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complex
A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to clinical isolates of Mycobacterium tuberculosis complex to reduce the cost of using lyticase. This protocol reduces the expense of PFGE typing of Mycobacterium tuberculosis complex as it removes the use of lyti...
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Tehran University of Medical Sciences
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doaj-b5058c0d308a4b11b8bd240de88c2cb42020-12-02T06:11:28ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472012-03-0141Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complexArash Ghodousi0S Vatani1Davood Darban-Sarokhalil2Maryam Omrani3AA Fooladi4Ad Khosaravi5Mohammad Mehdi Feizabadi6Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.ADept. of Microbiology, School of Medicine & Infectious and Tropical Diseases Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran..Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.Research Center of Molecular Biology, Department of Pathology, School of Medical Sciences, Baqiyatallah University of Medical Sciences, Tehran, Iran.ADept. of Microbiology, School of Medicine & Infectious and Tropical Diseases Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to clinical isolates of Mycobacterium tuberculosis complex to reduce the cost of using lyticase. This protocol reduces the expense of PFGE typing of Mycobacterium tuberculosis complex as it removes the use of lyticase during the spheroplast formation from these bacteria. https://ijm.tums.ac.ir/index.php/ijm/article/view/713Mycobacterium tuberculosisPulsed-filed gel electrophoresisTB-complex |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Arash Ghodousi S Vatani Davood Darban-Sarokhalil Maryam Omrani AA Fooladi Ad Khosaravi Mohammad Mehdi Feizabadi |
spellingShingle |
Arash Ghodousi S Vatani Davood Darban-Sarokhalil Maryam Omrani AA Fooladi Ad Khosaravi Mohammad Mehdi Feizabadi Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complex Iranian Journal of Microbiology Mycobacterium tuberculosis Pulsed-filed gel electrophoresis TB-complex |
author_facet |
Arash Ghodousi S Vatani Davood Darban-Sarokhalil Maryam Omrani AA Fooladi Ad Khosaravi Mohammad Mehdi Feizabadi |
author_sort |
Arash Ghodousi |
title |
Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complex |
title_short |
Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complex |
title_full |
Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complex |
title_fullStr |
Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complex |
title_full_unstemmed |
Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complex |
title_sort |
development of a new dna extraction protocol for pfge typing of mycobacterium tuberculosis complex |
publisher |
Tehran University of Medical Sciences |
series |
Iranian Journal of Microbiology |
issn |
2008-3289 2008-4447 |
publishDate |
2012-03-01 |
description |
A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to clinical isolates of Mycobacterium tuberculosis complex to reduce the cost of using lyticase. This protocol reduces the expense of PFGE typing of Mycobacterium tuberculosis complex as it removes the use of lyticase during the spheroplast formation from these bacteria.
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topic |
Mycobacterium tuberculosis Pulsed-filed gel electrophoresis TB-complex |
url |
https://ijm.tums.ac.ir/index.php/ijm/article/view/713 |
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