An In Vivo Requirement for the Mediator Subunit Med14 in the Maintenance of Stem Cell Populations
The Mediator complex has recently been shown to be a key player in the maintenance of embryonic and induced pluripotent stem cells. However, the in vivo consequences of loss of many Mediator subunits are unknown. We identified med14 as the gene affected in the zebrafish logelei (log) mutant, which d...
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doaj-b50d2b70e9494ff19da44237e6c01bce2020-11-24T22:40:45ZengElsevierStem Cell Reports2213-67112015-04-014467068410.1016/j.stemcr.2015.02.006An In Vivo Requirement for the Mediator Subunit Med14 in the Maintenance of Stem Cell PopulationsJeffrey T.A. Burrows0Bret J. Pearson1Ian C. Scott2Department of Molecular Genetics, University of Toronto, Toronto, ON M5S 1A8, CanadaDepartment of Molecular Genetics, University of Toronto, Toronto, ON M5S 1A8, CanadaDepartment of Molecular Genetics, University of Toronto, Toronto, ON M5S 1A8, CanadaThe Mediator complex has recently been shown to be a key player in the maintenance of embryonic and induced pluripotent stem cells. However, the in vivo consequences of loss of many Mediator subunits are unknown. We identified med14 as the gene affected in the zebrafish logelei (log) mutant, which displayed a morphological arrest by 2 days of development. Surprisingly, microarray analysis showed that transcription was not broadly affected in log mutants. Indeed, log cells transplanted into a wild-type environment were able to survive into adulthood. In planarians, RNAi knockdown demonstrated a requirement for med14 and many other Mediator components in adult stem cell maintenance and regeneration. Multiple stem/progenitor cell populations were observed to be reduced or absent in zebrafish med14 mutant embryos. Taken together, our results show a critical, evolutionarily conserved, in vivo function for Med14 (and Mediator) in stem cell maintenance, distinct from a general role in transcription.http://www.sciencedirect.com/science/article/pii/S2213671115000624 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jeffrey T.A. Burrows Bret J. Pearson Ian C. Scott |
spellingShingle |
Jeffrey T.A. Burrows Bret J. Pearson Ian C. Scott An In Vivo Requirement for the Mediator Subunit Med14 in the Maintenance of Stem Cell Populations Stem Cell Reports |
author_facet |
Jeffrey T.A. Burrows Bret J. Pearson Ian C. Scott |
author_sort |
Jeffrey T.A. Burrows |
title |
An In Vivo Requirement for the Mediator Subunit Med14 in the Maintenance of Stem Cell Populations |
title_short |
An In Vivo Requirement for the Mediator Subunit Med14 in the Maintenance of Stem Cell Populations |
title_full |
An In Vivo Requirement for the Mediator Subunit Med14 in the Maintenance of Stem Cell Populations |
title_fullStr |
An In Vivo Requirement for the Mediator Subunit Med14 in the Maintenance of Stem Cell Populations |
title_full_unstemmed |
An In Vivo Requirement for the Mediator Subunit Med14 in the Maintenance of Stem Cell Populations |
title_sort |
in vivo requirement for the mediator subunit med14 in the maintenance of stem cell populations |
publisher |
Elsevier |
series |
Stem Cell Reports |
issn |
2213-6711 |
publishDate |
2015-04-01 |
description |
The Mediator complex has recently been shown to be a key player in the maintenance of embryonic and induced pluripotent stem cells. However, the in vivo consequences of loss of many Mediator subunits are unknown. We identified med14 as the gene affected in the zebrafish logelei (log) mutant, which displayed a morphological arrest by 2 days of development. Surprisingly, microarray analysis showed that transcription was not broadly affected in log mutants. Indeed, log cells transplanted into a wild-type environment were able to survive into adulthood. In planarians, RNAi knockdown demonstrated a requirement for med14 and many other Mediator components in adult stem cell maintenance and regeneration. Multiple stem/progenitor cell populations were observed to be reduced or absent in zebrafish med14 mutant embryos. Taken together, our results show a critical, evolutionarily conserved, in vivo function for Med14 (and Mediator) in stem cell maintenance, distinct from a general role in transcription. |
url |
http://www.sciencedirect.com/science/article/pii/S2213671115000624 |
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