Experimental inoculation of Treponema pedis T A4 failed to induce ear necrosis in pigs
Abstract Ear necrosis is a syndrome affecting pigs shortly after weaning and is regarded as an animal welfare issue. The etiology is unknown but Treponema spp., predominantly Treponema pedis, are commonly detected in the lesions. Oral treponemes have been suggested as source of infection, transferre...
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doaj-b511273bf7f34a56814e71d967d34ac92020-11-25T01:04:20ZengBMCPorcine Health Management2055-56602017-12-01311510.1186/s40813-017-0073-2Experimental inoculation of Treponema pedis T A4 failed to induce ear necrosis in pigsFrida Karlsson0Anna Rosander1Claes Fellström2Annette Backhans3Farm and Animal HealthDepartment of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural SciencesDepartment of Clinical Sciences, Swedish University of Agricultural SciencesDepartment of Clinical Sciences, Swedish University of Agricultural SciencesAbstract Ear necrosis is a syndrome affecting pigs shortly after weaning and is regarded as an animal welfare issue. The etiology is unknown but Treponema spp., predominantly Treponema pedis, are commonly detected in the lesions. Oral treponemes have been suggested as source of infection, transferred by biting and licking behavior. In this study, five pigs were intradermally inoculated with Treponema pedis strain T A4 with the aim of investigating if this strain would induce ear lesions. Three pigs served as controls. The inoculation was repeated after 29 days, and the study continued for 56 days. Serum samples were collected throughout the study and analyzed by ELISA for IgG antibodies towards T. pedis T A4 lysate. Skin biopsies were taken from the inoculation area at the end of the study. Gingival samples were collected and cultivated for treponemes, for comparison to the inoculation strain and to follow colonisation. The challenged pigs did not develop any clinical signs of infection and no spirochetes were detected in sections from skin biopsies. The number of Treponema-positive gingival samples increased during the study. In the challenge group, IgG towards the bacterial lysate peaked 7 days after each inoculation and decreased rapidly hereafter. In the control group a weak IgG response was observed after the second inoculation, possibly caused by the oral treponemes.http://link.springer.com/article/10.1186/s40813-017-0073-2PigEar necrosisTreponemaIgGExperimental infection |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Frida Karlsson Anna Rosander Claes Fellström Annette Backhans |
spellingShingle |
Frida Karlsson Anna Rosander Claes Fellström Annette Backhans Experimental inoculation of Treponema pedis T A4 failed to induce ear necrosis in pigs Porcine Health Management Pig Ear necrosis Treponema IgG Experimental infection |
author_facet |
Frida Karlsson Anna Rosander Claes Fellström Annette Backhans |
author_sort |
Frida Karlsson |
title |
Experimental inoculation of Treponema pedis T A4 failed to induce ear necrosis in pigs |
title_short |
Experimental inoculation of Treponema pedis T A4 failed to induce ear necrosis in pigs |
title_full |
Experimental inoculation of Treponema pedis T A4 failed to induce ear necrosis in pigs |
title_fullStr |
Experimental inoculation of Treponema pedis T A4 failed to induce ear necrosis in pigs |
title_full_unstemmed |
Experimental inoculation of Treponema pedis T A4 failed to induce ear necrosis in pigs |
title_sort |
experimental inoculation of treponema pedis t a4 failed to induce ear necrosis in pigs |
publisher |
BMC |
series |
Porcine Health Management |
issn |
2055-5660 |
publishDate |
2017-12-01 |
description |
Abstract Ear necrosis is a syndrome affecting pigs shortly after weaning and is regarded as an animal welfare issue. The etiology is unknown but Treponema spp., predominantly Treponema pedis, are commonly detected in the lesions. Oral treponemes have been suggested as source of infection, transferred by biting and licking behavior. In this study, five pigs were intradermally inoculated with Treponema pedis strain T A4 with the aim of investigating if this strain would induce ear lesions. Three pigs served as controls. The inoculation was repeated after 29 days, and the study continued for 56 days. Serum samples were collected throughout the study and analyzed by ELISA for IgG antibodies towards T. pedis T A4 lysate. Skin biopsies were taken from the inoculation area at the end of the study. Gingival samples were collected and cultivated for treponemes, for comparison to the inoculation strain and to follow colonisation. The challenged pigs did not develop any clinical signs of infection and no spirochetes were detected in sections from skin biopsies. The number of Treponema-positive gingival samples increased during the study. In the challenge group, IgG towards the bacterial lysate peaked 7 days after each inoculation and decreased rapidly hereafter. In the control group a weak IgG response was observed after the second inoculation, possibly caused by the oral treponemes. |
topic |
Pig Ear necrosis Treponema IgG Experimental infection |
url |
http://link.springer.com/article/10.1186/s40813-017-0073-2 |
work_keys_str_mv |
AT fridakarlsson experimentalinoculationoftreponemapedista4failedtoinduceearnecrosisinpigs AT annarosander experimentalinoculationoftreponemapedista4failedtoinduceearnecrosisinpigs AT claesfellstrom experimentalinoculationoftreponemapedista4failedtoinduceearnecrosisinpigs AT annettebackhans experimentalinoculationoftreponemapedista4failedtoinduceearnecrosisinpigs |
_version_ |
1725198760453079040 |