Cytokines in the colon, central nervous system and serum of irritable bowel syndrome rats
Abstract Objective The aim of this study was to detect the expression of interleukin (IL)-1β and transforming growth factor (TGF)-β1 in the colonic tissue and serum of irritable bowel syndrome (IBS) rats, as well as the distribution and expression of corticotropin-releasing factor (CRF) in the spina...
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doaj-b51c1c7c456a41a09b2e9308103227ee2021-01-17T12:54:44ZengBMCEuropean Journal of Medical Research2047-783X2021-01-012611710.1186/s40001-021-00479-wCytokines in the colon, central nervous system and serum of irritable bowel syndrome ratsGuanqun Chao0Zhaojun Wang1Xinli Chen2Shuo Zhang3Department of Family Medicine, Sir Run Run Shaw Hospital, Zhejiang UniversityDepartment of Gastroenterology, The First Affiliated Hospital, Zhejiang Chinese Medical UniversityDepartment of Gastroenterology, The First Affiliated Hospital, Zhejiang Chinese Medical UniversityDepartment of Gastroenterology, The First Affiliated Hospital, Zhejiang Chinese Medical UniversityAbstract Objective The aim of this study was to detect the expression of interleukin (IL)-1β and transforming growth factor (TGF)-β1 in the colonic tissue and serum of irritable bowel syndrome (IBS) rats, as well as the distribution and expression of corticotropin-releasing factor (CRF) in the spinal cord and brain of the visceral hypersensitivity rats, thus to ascertain the mechanism of visceral hypersensitivity signal conduction pathway. Methods The expression of IL-1β and TGF-β1 in the colonic tissue and serum of IBS rats was screened by the liquid chip technology and verified by RT-PCR technology. Then the quantitative analysis of CRF in the spinal cord and brain was achieved by the immunohistochemical method and computerized image system. Result The rat model with visceral hypersensitivity was successfully established. Among the screened indicators of IL-1β and TGF-β1 in colon tissue and serum, only the expression of IL-1β in the model group was up-regulated (P < 0.05). The immunohistochemical method showed that CRF was expressed in the spinal cord, hypothalamus, and the third ventricle. The positive index number of the model groups was higher than that of the control group (P < 0.01). Conclusion From the research, it can be inferred that IL-1β may participate in the pathogenesis mechanism of IBS via regulating the colon function. The increasing expression of CRF linked to stress in the spinal cord, hypothalamus and the third ventricle indicated that it might play an important role in the mechanisms of visceral hypersensitivity signal conduction pathway.https://doi.org/10.1186/s40001-021-00479-wIrritable bowel syndromeColonic mucosaIL-1βTGF-β1CRF |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Guanqun Chao Zhaojun Wang Xinli Chen Shuo Zhang |
spellingShingle |
Guanqun Chao Zhaojun Wang Xinli Chen Shuo Zhang Cytokines in the colon, central nervous system and serum of irritable bowel syndrome rats European Journal of Medical Research Irritable bowel syndrome Colonic mucosa IL-1β TGF-β1 CRF |
author_facet |
Guanqun Chao Zhaojun Wang Xinli Chen Shuo Zhang |
author_sort |
Guanqun Chao |
title |
Cytokines in the colon, central nervous system and serum of irritable bowel syndrome rats |
title_short |
Cytokines in the colon, central nervous system and serum of irritable bowel syndrome rats |
title_full |
Cytokines in the colon, central nervous system and serum of irritable bowel syndrome rats |
title_fullStr |
Cytokines in the colon, central nervous system and serum of irritable bowel syndrome rats |
title_full_unstemmed |
Cytokines in the colon, central nervous system and serum of irritable bowel syndrome rats |
title_sort |
cytokines in the colon, central nervous system and serum of irritable bowel syndrome rats |
publisher |
BMC |
series |
European Journal of Medical Research |
issn |
2047-783X |
publishDate |
2021-01-01 |
description |
Abstract Objective The aim of this study was to detect the expression of interleukin (IL)-1β and transforming growth factor (TGF)-β1 in the colonic tissue and serum of irritable bowel syndrome (IBS) rats, as well as the distribution and expression of corticotropin-releasing factor (CRF) in the spinal cord and brain of the visceral hypersensitivity rats, thus to ascertain the mechanism of visceral hypersensitivity signal conduction pathway. Methods The expression of IL-1β and TGF-β1 in the colonic tissue and serum of IBS rats was screened by the liquid chip technology and verified by RT-PCR technology. Then the quantitative analysis of CRF in the spinal cord and brain was achieved by the immunohistochemical method and computerized image system. Result The rat model with visceral hypersensitivity was successfully established. Among the screened indicators of IL-1β and TGF-β1 in colon tissue and serum, only the expression of IL-1β in the model group was up-regulated (P < 0.05). The immunohistochemical method showed that CRF was expressed in the spinal cord, hypothalamus, and the third ventricle. The positive index number of the model groups was higher than that of the control group (P < 0.01). Conclusion From the research, it can be inferred that IL-1β may participate in the pathogenesis mechanism of IBS via regulating the colon function. The increasing expression of CRF linked to stress in the spinal cord, hypothalamus and the third ventricle indicated that it might play an important role in the mechanisms of visceral hypersensitivity signal conduction pathway. |
topic |
Irritable bowel syndrome Colonic mucosa IL-1β TGF-β1 CRF |
url |
https://doi.org/10.1186/s40001-021-00479-w |
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