Antioxidative Properties of Defatted Dabai Pulp and Peel Prepared by Solid Phase Extraction

Solid phase extraction (SPE) using Sep-Pak<sup>®</sup> cartridges is one of the techniques used for fractionation of antioxidant compounds in waste of dabai oil extraction (defatted dabai parts). The aim of this study was to determine the phenolic compounds and antioxidan...

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Main Authors: Faridah Abas, Amin Ismail, Azrina Azlan, Hock Eng Khoo
Format: Article
Language:English
Published: MDPI AG 2012-08-01
Series:Molecules
Subjects:
Online Access:http://www.mdpi.com/1420-3049/17/8/9754
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spelling doaj-b51c657403c449e98a7dfd1fb94fe8ac2020-11-25T00:19:59ZengMDPI AGMolecules1420-30492012-08-011789754977310.3390/molecules17089754Antioxidative Properties of Defatted Dabai Pulp and Peel Prepared by Solid Phase ExtractionFaridah AbasAmin IsmailAzrina AzlanHock Eng KhooSolid phase extraction (SPE) using Sep-Pak<sup>®</sup> cartridges is one of the techniques used for fractionation of antioxidant compounds in waste of dabai oil extraction (defatted dabai parts). The aim of this study was to determine the phenolic compounds and antioxidant capacity in crude extracts and several SPE fractions from methanolic extract of defatted dabai pulp and peel. Based on SPE, Sep-Pak<sup>®</sup> cyanopropyl and C<sub>18</sub> cartridges were used to fractionate the antioxidant-rich crude extracts into water and methanolic fractions. Analyzed using LC-MS, flavonoids, anthocyanins, saponin derivatives and other unknown antioxidative compounds were detected in the defatted dabai crude extracts and their SPE fractions. Anthocyanins were the major phenolic compounds identified in the defatted dabai peel and detected in most of the SPE fractions. Methanolic fractions of defatted dabai parts embraced higher total phenolics and antioxidant capacity than water fractions. This finding also revealed the crude extracts of defatted dabai peel have the most significant antioxidant properties compared to the methanolic and water fractions studied. The crude extract of defatted dabai parts remain as the most potent antioxidant as it contains mixture of flavonoids, anthocyanins and other potential antioxidants.http://www.mdpi.com/1420-3049/17/8/9754anthocyaninantioxidant<em> </em>dabaimass spectrometrysolid phase extraction
collection DOAJ
language English
format Article
sources DOAJ
author Faridah Abas
Amin Ismail
Azrina Azlan
Hock Eng Khoo
spellingShingle Faridah Abas
Amin Ismail
Azrina Azlan
Hock Eng Khoo
Antioxidative Properties of Defatted Dabai Pulp and Peel Prepared by Solid Phase Extraction
Molecules
anthocyanin
antioxidant
<em> </em>dabai
mass spectrometry
solid phase extraction
author_facet Faridah Abas
Amin Ismail
Azrina Azlan
Hock Eng Khoo
author_sort Faridah Abas
title Antioxidative Properties of Defatted Dabai Pulp and Peel Prepared by Solid Phase Extraction
title_short Antioxidative Properties of Defatted Dabai Pulp and Peel Prepared by Solid Phase Extraction
title_full Antioxidative Properties of Defatted Dabai Pulp and Peel Prepared by Solid Phase Extraction
title_fullStr Antioxidative Properties of Defatted Dabai Pulp and Peel Prepared by Solid Phase Extraction
title_full_unstemmed Antioxidative Properties of Defatted Dabai Pulp and Peel Prepared by Solid Phase Extraction
title_sort antioxidative properties of defatted dabai pulp and peel prepared by solid phase extraction
publisher MDPI AG
series Molecules
issn 1420-3049
publishDate 2012-08-01
description Solid phase extraction (SPE) using Sep-Pak<sup>®</sup> cartridges is one of the techniques used for fractionation of antioxidant compounds in waste of dabai oil extraction (defatted dabai parts). The aim of this study was to determine the phenolic compounds and antioxidant capacity in crude extracts and several SPE fractions from methanolic extract of defatted dabai pulp and peel. Based on SPE, Sep-Pak<sup>®</sup> cyanopropyl and C<sub>18</sub> cartridges were used to fractionate the antioxidant-rich crude extracts into water and methanolic fractions. Analyzed using LC-MS, flavonoids, anthocyanins, saponin derivatives and other unknown antioxidative compounds were detected in the defatted dabai crude extracts and their SPE fractions. Anthocyanins were the major phenolic compounds identified in the defatted dabai peel and detected in most of the SPE fractions. Methanolic fractions of defatted dabai parts embraced higher total phenolics and antioxidant capacity than water fractions. This finding also revealed the crude extracts of defatted dabai peel have the most significant antioxidant properties compared to the methanolic and water fractions studied. The crude extract of defatted dabai parts remain as the most potent antioxidant as it contains mixture of flavonoids, anthocyanins and other potential antioxidants.
topic anthocyanin
antioxidant
<em> </em>dabai
mass spectrometry
solid phase extraction
url http://www.mdpi.com/1420-3049/17/8/9754
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