Stable and Local Reservoirs of Mycobacterium ulcerans Inferred from the Nonrandom Distribution of Bacterial Genotypes, Benin

• Main Authors: Clément Coudereau, Alban Besnard, Marie Robbe-Saule, Céline Bris, Marie Kempf, Roch Christian Johnson, Télésphore Yao Brou, Ronald Gnimavo, Sara Eyangoh, Fida Khater, Estelle Marion
• Format: Article
• Language: English
• Published: Centers for Disease Control and Prevention 2020-03-01
• Series: Emerging Infectious Diseases
• Subjects: Mycobacterium ulcerans; Buruli ulcer; genetic diversity; mutational profiling; genomic analysis; geospatial model
• Online Access: https://wwwnc.cdc.gov/eid/article/26/3/19-0573_article

Mycobacterium ulcerans is the causative agent of Buruli ulcer, a neglected tropical disease found in rural areas of West and Central Africa. Despite the ongoing efforts to tackle Buruli ulcer epidemics, the environmental reservoir of its pathogen remains elusive, underscoring the need for new approaches to improving disease prevention and management. In our study, we implemented a local-scale spatial clustering model and deciphered the genetic diversity of the bacteria in a small area of Benin where Buruli ulcer is endemic. Using 179 strain samples from West Africa, we conducted a phylogeographic analysis combining whole-genome sequencing with spatial scan statistics. The 8 distinct genotypes we identified were by no means randomly spread over the studied area. Instead, they were divided into 3 different geographic clusters, associated with landscape characteristics. Our results highlight the ability of M. ulcerans to evolve independently and differentially depending on location in a specific ecologic reservoir.