Differences in gene expression and molecular pathway regulation between MYCN amplified and 2p gain neuroblastoma tumors
OBJECTIVE: The malignancy of neuroblastoma (NB) is strongly connected with MYCN oncogene status. The gene expression profile was investigated in three subtypes of NB related to MYCN status (amplification - MNA, 2p gain and normal) in order to identify new candidate genes and to elucidate development...
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doaj-b538c435775c4e88b670ffe206dad7562021-08-02T15:19:52ZengVerduci EditoreWorld Cancer Research Journal2372-34162020-09-01710.32113/wcrj_20209_16751675Differences in gene expression and molecular pathway regulation between MYCN amplified and 2p gain neuroblastoma tumorsK. Szewczyk0Department of Medical Genetics, Faculty of Medicine, Jagiellonian University Medical College, Krakow, PolandOBJECTIVE: The malignancy of neuroblastoma (NB) is strongly connected with MYCN oncogene status. The gene expression profile was investigated in three subtypes of NB related to MYCN status (amplification - MNA, 2p gain and normal) in order to identify new candidate genes and to elucidate development of more aggressive forms of this pediatric tumor. MATERIALS AND METHODS: Human whole genome oligonucleotide expression microarrays were applied in the study. RESULTS: Hierarchical clustering analysis presented two distinct gene expression patterns corresponding to cases with and without MNA. For the first time, the 7 most upregulated genes and the 13 most downregulated genes in the MNA subgroup were selected in comparison to 2p gain tumors. CONCLUSIONS: The obtained result demonstrates that MYCN has a significant impact on genome-wide NB gene expression. Increasing MYCN level promotes cell growth and motility while counteracting differentiation and attachment. Interestingly, NB with 2p gain, in comparison to MNA and normal MYCN status, showed a higher expression level of genes involved in cell migration but downregulated genes involved in nervous system development. This finding may indicate that 2p gain tumors have more aggressive behavior with a higher tendency toward metastasis than MNA cases.https://www.wcrj.net/wp-content/uploads/sites/5/2020/11/e1675.pdf2p gaingene expressionmolecular pathwaymycn amplificationneuroblastoma |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
K. Szewczyk |
spellingShingle |
K. Szewczyk Differences in gene expression and molecular pathway regulation between MYCN amplified and 2p gain neuroblastoma tumors World Cancer Research Journal 2p gain gene expression molecular pathway mycn amplification neuroblastoma |
author_facet |
K. Szewczyk |
author_sort |
K. Szewczyk |
title |
Differences in gene expression and molecular pathway regulation between MYCN amplified and 2p gain neuroblastoma tumors |
title_short |
Differences in gene expression and molecular pathway regulation between MYCN amplified and 2p gain neuroblastoma tumors |
title_full |
Differences in gene expression and molecular pathway regulation between MYCN amplified and 2p gain neuroblastoma tumors |
title_fullStr |
Differences in gene expression and molecular pathway regulation between MYCN amplified and 2p gain neuroblastoma tumors |
title_full_unstemmed |
Differences in gene expression and molecular pathway regulation between MYCN amplified and 2p gain neuroblastoma tumors |
title_sort |
differences in gene expression and molecular pathway regulation between mycn amplified and 2p gain neuroblastoma tumors |
publisher |
Verduci Editore |
series |
World Cancer Research Journal |
issn |
2372-3416 |
publishDate |
2020-09-01 |
description |
OBJECTIVE: The malignancy of neuroblastoma (NB) is strongly connected with MYCN oncogene status. The gene expression profile was investigated in three subtypes of NB related to MYCN status (amplification - MNA, 2p gain and normal) in order to identify new candidate genes and to elucidate development of more aggressive forms of this pediatric tumor.
MATERIALS AND METHODS: Human whole genome oligonucleotide expression microarrays were applied in the study.
RESULTS: Hierarchical clustering analysis presented two distinct gene expression patterns corresponding to cases with and without MNA. For the first time, the 7 most upregulated genes and the 13 most downregulated genes in the MNA subgroup were selected in comparison to 2p gain tumors.
CONCLUSIONS: The obtained result demonstrates that MYCN has a significant impact on genome-wide NB gene expression. Increasing MYCN level promotes cell growth and motility while counteracting differentiation and attachment. Interestingly, NB with 2p gain, in comparison to MNA and normal MYCN status, showed a higher expression level of genes involved in cell migration but downregulated genes involved in nervous system development. This finding may indicate that 2p gain tumors have more aggressive behavior with a higher tendency toward metastasis than MNA cases. |
topic |
2p gain gene expression molecular pathway mycn amplification neuroblastoma |
url |
https://www.wcrj.net/wp-content/uploads/sites/5/2020/11/e1675.pdf |
work_keys_str_mv |
AT kszewczyk differencesingeneexpressionandmolecularpathwayregulationbetweenmycnamplifiedand2pgainneuroblastomatumors |
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