Analysis of insertion/deletion polymorphisms of the angiotensin converting enzyme gene in Malaysian end-stage renal disease patients
Introduction: Insertion/deletion (I/D) polymorphisms found in the angiotensin converting enzyme (ACE) gene have been associated with hypertension, diabetes and renal disease. The present study sought to determine the association of I/D polymorphisms of the ACE gene with end-stage renal disease (ESRD...
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doaj-b53b35287979434baafbd61ade2106dd2021-05-02T20:50:43ZengHindawi - SAGE PublishingJournal of the Renin-Angiotensin-Aldosterone System1470-32031752-89762015-12-011610.1177/1470320310392096Analysis of insertion/deletion polymorphisms of the angiotensin converting enzyme gene in Malaysian end-stage renal disease patientsAisyah Ali0Ramachandran Vasudevan1Patimah Ismail2Christoper Lim Thiam Seong3Srikumar Chakravarthi4Molecular Biology and Bioinformatics Lab, Department of Biomedical Sciences, Universiti Putra Malaysia, MalaysiaMolecular Biology and Bioinformatics Lab, Department of Biomedical Sciences, Universiti Putra Malaysia, MalaysiaMolecular Biology and Bioinformatics Lab, Department of Biomedical Sciences, Universiti Putra Malaysia, MalaysiaDepartment of Medicine, Universiti Putra Malaysia, MalaysiaFaculty of Medicine and Health Sciences, Department of Pathology, International Medical University, MalaysiaIntroduction: Insertion/deletion (I/D) polymorphisms found in the angiotensin converting enzyme (ACE) gene have been associated with hypertension, diabetes and renal disease. The present study sought to determine the association of I/D polymorphisms of the ACE gene with end-stage renal disease (ESRD) patients in Malaysia. Materials and methods: A total of 380 subjects were recruited to determine the genotypes of I/D polymorphisms of the ACE gene. Genotyping was performed using a PCR method. Statistical analyses were carried out using statistical software, and a level of p < 0.05 was considered statistically significant. Results: The frequencies for II, ID and DD genotypes of the ACE gene were 24.7%, 65.80% and 9.47%, respectively, in ESRD patients, and in control subjects were 45.26%, 47.37% and 7.37% respectively. The frequency for the D allele was found to be higher (42.40%) in ESRD patients compared to control subjects (31.05%). The genotypic and allelic frequencies of I/D polymorphisms of the ACE gene differed significantly ( p < 0.05) between ESRD patients and control subjects in the Malaysian population. Conclusion: The findings of this study indicate that I/D polymorphisms of the ACE gene are a useful marker and are likely to play a major role in determining genetic susceptibility to ESRD in the Malaysian population.https://doi.org/10.1177/1470320310392096 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Aisyah Ali Ramachandran Vasudevan Patimah Ismail Christoper Lim Thiam Seong Srikumar Chakravarthi |
spellingShingle |
Aisyah Ali Ramachandran Vasudevan Patimah Ismail Christoper Lim Thiam Seong Srikumar Chakravarthi Analysis of insertion/deletion polymorphisms of the angiotensin converting enzyme gene in Malaysian end-stage renal disease patients Journal of the Renin-Angiotensin-Aldosterone System |
author_facet |
Aisyah Ali Ramachandran Vasudevan Patimah Ismail Christoper Lim Thiam Seong Srikumar Chakravarthi |
author_sort |
Aisyah Ali |
title |
Analysis of insertion/deletion polymorphisms of the angiotensin converting enzyme gene in Malaysian end-stage renal disease patients |
title_short |
Analysis of insertion/deletion polymorphisms of the angiotensin converting enzyme gene in Malaysian end-stage renal disease patients |
title_full |
Analysis of insertion/deletion polymorphisms of the angiotensin converting enzyme gene in Malaysian end-stage renal disease patients |
title_fullStr |
Analysis of insertion/deletion polymorphisms of the angiotensin converting enzyme gene in Malaysian end-stage renal disease patients |
title_full_unstemmed |
Analysis of insertion/deletion polymorphisms of the angiotensin converting enzyme gene in Malaysian end-stage renal disease patients |
title_sort |
analysis of insertion/deletion polymorphisms of the angiotensin converting enzyme gene in malaysian end-stage renal disease patients |
publisher |
Hindawi - SAGE Publishing |
series |
Journal of the Renin-Angiotensin-Aldosterone System |
issn |
1470-3203 1752-8976 |
publishDate |
2015-12-01 |
description |
Introduction: Insertion/deletion (I/D) polymorphisms found in the angiotensin converting enzyme (ACE) gene have been associated with hypertension, diabetes and renal disease. The present study sought to determine the association of I/D polymorphisms of the ACE gene with end-stage renal disease (ESRD) patients in Malaysia. Materials and methods: A total of 380 subjects were recruited to determine the genotypes of I/D polymorphisms of the ACE gene. Genotyping was performed using a PCR method. Statistical analyses were carried out using statistical software, and a level of p < 0.05 was considered statistically significant. Results: The frequencies for II, ID and DD genotypes of the ACE gene were 24.7%, 65.80% and 9.47%, respectively, in ESRD patients, and in control subjects were 45.26%, 47.37% and 7.37% respectively. The frequency for the D allele was found to be higher (42.40%) in ESRD patients compared to control subjects (31.05%). The genotypic and allelic frequencies of I/D polymorphisms of the ACE gene differed significantly ( p < 0.05) between ESRD patients and control subjects in the Malaysian population. Conclusion: The findings of this study indicate that I/D polymorphisms of the ACE gene are a useful marker and are likely to play a major role in determining genetic susceptibility to ESRD in the Malaysian population. |
url |
https://doi.org/10.1177/1470320310392096 |
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