Production of Poly-α-hydroxybutyrate (PHB) from Sago Starch by The Native Isolate Bacillus megaterium PSA10

Production of Poly-α-hydroxybutyrate (PHB) from Sago Starch by The Native Isolate Bacillus megaterium PSA10

A new bacterial strain that produces amylase and poly-α-hidroxybutyrate (PHB) using sago starch as carbon source was characterized and identified to be member of the Bacillus megaterium group based on phenotypic characteristics and 16S rDNA gene sequences. Amylase activity was determin...

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Main Authors: Nur Arfa Yanti, Langkah Sembiring, Sebastian Margino
Format: Article
Language:English
Published: Universitas Gadjah Mada, Yogyakarta 2015-10-01
Series:Indonesian Journal of Biotechnology
Online Access:http://journal.ugm.ac.id/ijbiotech/article/view/7559
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spelling doaj-b541418aaed648db9541ebc7885e66f52020-11-24T21:56:32ZengUniversitas Gadjah Mada, YogyakartaIndonesian Journal of Biotechnology0853-86542089-22412015-10-011116360Production of Poly-α-hydroxybutyrate (PHB) from Sago Starch by The Native Isolate Bacillus megaterium PSA10Nur Arfa YantiLangkah SembiringSebastian MarginoA new bacterial strain that produces amylase and poly-α-hidroxybutyrate (PHB) using sago starch as carbon source was characterized and identified to be member of the Bacillus megaterium group based on phenotypic characteristics and 16S rDNA gene sequences. Amylase activity was determined spectrophotometrically on the basis of substrate concentration reduction. PHB production was quantified with UV spectrophotometer. The polymer produced by B. megaterium PSA10 was identified by Fourier Transform Infrared spectroscopy (FTIR). The result of the study showed that the amylase specific activity B. megaterium PSA10 was 593,61 DUN/mg protein and PHB production from sago starch was 52,28 % of cell dry weight (CDW). FTIR analysis of the polymer indicated that the strain B.megaterium PSA10 was a potent PHB producer.http://journal.ugm.ac.id/ijbiotech/article/view/7559
collection DOAJ
language English
format Article
sources DOAJ
author Nur Arfa Yanti
Langkah Sembiring
Sebastian Margino
spellingShingle Nur Arfa Yanti
Langkah Sembiring
Sebastian Margino
Production of Poly-α-hydroxybutyrate (PHB) from Sago Starch by The Native Isolate Bacillus megaterium PSA10
Indonesian Journal of Biotechnology
author_facet Nur Arfa Yanti
Langkah Sembiring
Sebastian Margino
author_sort Nur Arfa Yanti
title Production of Poly-α-hydroxybutyrate (PHB) from Sago Starch by The Native Isolate Bacillus megaterium PSA10
title_short Production of Poly-α-hydroxybutyrate (PHB) from Sago Starch by The Native Isolate Bacillus megaterium PSA10
title_full Production of Poly-α-hydroxybutyrate (PHB) from Sago Starch by The Native Isolate Bacillus megaterium PSA10
title_fullStr Production of Poly-α-hydroxybutyrate (PHB) from Sago Starch by The Native Isolate Bacillus megaterium PSA10
title_full_unstemmed Production of Poly-α-hydroxybutyrate (PHB) from Sago Starch by The Native Isolate Bacillus megaterium PSA10
title_sort production of poly-î±-hydroxybutyrate (phb) from sago starch by the native isolate bacillus megaterium psa10
publisher Universitas Gadjah Mada, Yogyakarta
series Indonesian Journal of Biotechnology
issn 0853-8654
2089-2241
publishDate 2015-10-01
description A new bacterial strain that produces amylase and poly-α-hidroxybutyrate (PHB) using sago starch as carbon source was characterized and identified to be member of the Bacillus megaterium group based on phenotypic characteristics and 16S rDNA gene sequences. Amylase activity was determined spectrophotometrically on the basis of substrate concentration reduction. PHB production was quantified with UV spectrophotometer. The polymer produced by B. megaterium PSA10 was identified by Fourier Transform Infrared spectroscopy (FTIR). The result of the study showed that the amylase specific activity B. megaterium PSA10 was 593,61 DUN/mg protein and PHB production from sago starch was 52,28 % of cell dry weight (CDW). FTIR analysis of the polymer indicated that the strain B.megaterium PSA10 was a potent PHB producer.
url http://journal.ugm.ac.id/ijbiotech/article/view/7559
work_keys_str_mv AT nurarfayanti productionofpolyihydroxybutyratephbfromsagostarchbythenativeisolatebacillusmegateriumpsa10
AT langkahsembiring productionofpolyihydroxybutyratephbfromsagostarchbythenativeisolatebacillusmegateriumpsa10
AT sebastianmargino productionofpolyihydroxybutyratephbfromsagostarchbythenativeisolatebacillusmegateriumpsa10
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