Biotransformation of Ginsenoside Rf to Rh1 by Recombinant β-Glucosidase

Biotransformation of Ginsenoside Rf to Rh1 by Recombinant β-Glucosidase

An Aspergillus niger strain was isolated from the soil around ginseng fruit. In vitro enzyme assays showed that this strain had the ability to transform total ginsenosides (TGS) into several new products. In a further biochemical study, a β-glucosidase gene isolated from this strain, bgl1, was expre...

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Main Authors: Yi-Nan Zheng, Xiang Li, Hong-Yu Pan, Yu-Xia Qin, Jun Lei, Lian-Xue Zhang, Zhi Liu, Guang-Zhi Sun, Chang-Chun Ruan, Hao Zhang
Format: Article
Language:English
Published: MDPI AG 2009-06-01
Series:Molecules
Subjects:
Aspergillus niger
β-glucosidase
ginsenoside Rf
ginsenoside Rh1
Online Access:http://www.mdpi.com/1420-3049/14/6/2043/
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spelling doaj-b54fc1d099554139a2f2b38f15c2886d2020-11-24T20:58:00ZengMDPI AGMolecules1420-30492009-06-011462043204810.3390/molecules14062043Biotransformation of Ginsenoside Rf to Rh1 by Recombinant β-GlucosidaseYi-Nan ZhengXiang LiHong-Yu PanYu-Xia QinJun LeiLian-Xue ZhangZhi LiuGuang-Zhi SunChang-Chun RuanHao ZhangAn Aspergillus niger strain was isolated from the soil around ginseng fruit. In vitro enzyme assays showed that this strain had the ability to transform total ginsenosides (TGS) into several new products. In a further biochemical study, a β-glucosidase gene isolated from this strain, bgl1, was expressed in Saccharomyces cerevisiae. His-tagged BGL1 protein (~170 kD) showed the ability to transform ginsenoside Rf into Rh1. http://www.mdpi.com/1420-3049/14/6/2043/Aspergillus nigerβ-glucosidaseginsenoside Rfginsenoside Rh1
collection DOAJ
language English
format Article
sources DOAJ
author Yi-Nan Zheng
Xiang Li
Hong-Yu Pan
Yu-Xia Qin
Jun Lei
Lian-Xue Zhang
Zhi Liu
Guang-Zhi Sun
Chang-Chun Ruan
Hao Zhang
spellingShingle Yi-Nan Zheng
Xiang Li
Hong-Yu Pan
Yu-Xia Qin
Jun Lei
Lian-Xue Zhang
Zhi Liu
Guang-Zhi Sun
Chang-Chun Ruan
Hao Zhang
Biotransformation of Ginsenoside Rf to Rh1 by Recombinant β-Glucosidase
Molecules
Aspergillus niger
β-glucosidase
ginsenoside Rf
ginsenoside Rh1
author_facet Yi-Nan Zheng
Xiang Li
Hong-Yu Pan
Yu-Xia Qin
Jun Lei
Lian-Xue Zhang
Zhi Liu
Guang-Zhi Sun
Chang-Chun Ruan
Hao Zhang
author_sort Yi-Nan Zheng
title Biotransformation of Ginsenoside Rf to Rh1 by Recombinant β-Glucosidase
title_short Biotransformation of Ginsenoside Rf to Rh1 by Recombinant β-Glucosidase
title_full Biotransformation of Ginsenoside Rf to Rh1 by Recombinant β-Glucosidase
title_fullStr Biotransformation of Ginsenoside Rf to Rh1 by Recombinant β-Glucosidase
title_full_unstemmed Biotransformation of Ginsenoside Rf to Rh1 by Recombinant β-Glucosidase
title_sort biotransformation of ginsenoside rf to rh1 by recombinant β-glucosidase
publisher MDPI AG
series Molecules
issn 1420-3049
publishDate 2009-06-01
description An Aspergillus niger strain was isolated from the soil around ginseng fruit. In vitro enzyme assays showed that this strain had the ability to transform total ginsenosides (TGS) into several new products. In a further biochemical study, a β-glucosidase gene isolated from this strain, bgl1, was expressed in Saccharomyces cerevisiae. His-tagged BGL1 protein (~170 kD) showed the ability to transform ginsenoside Rf into Rh1.
topic Aspergillus niger
β-glucosidase
ginsenoside Rf
ginsenoside Rh1
url http://www.mdpi.com/1420-3049/14/6/2043/
work_keys_str_mv AT yinanzheng biotransformationofginsenosiderftorh1byrecombinantbglucosidase
AT xiangli biotransformationofginsenosiderftorh1byrecombinantbglucosidase
AT hongyupan biotransformationofginsenosiderftorh1byrecombinantbglucosidase
AT yuxiaqin biotransformationofginsenosiderftorh1byrecombinantbglucosidase
AT junlei biotransformationofginsenosiderftorh1byrecombinantbglucosidase
AT lianxuezhang biotransformationofginsenosiderftorh1byrecombinantbglucosidase
AT zhiliu biotransformationofginsenosiderftorh1byrecombinantbglucosidase
AT guangzhisun biotransformationofginsenosiderftorh1byrecombinantbglucosidase
AT changchunruan biotransformationofginsenosiderftorh1byrecombinantbglucosidase
AT haozhang biotransformationofginsenosiderftorh1byrecombinantbglucosidase
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