Refinement of microbiota analysis of specimens from patients with respiratory infections using next-generation sequencing
Abstract Next-generation sequencing (NGS) technologies have been applied in bacterial flora analysis. However, there is no standardized protocol, and the optimal clustering threshold for estimating bacterial species in respiratory infection specimens is unknown. This study was conducted to investiga...
Main Authors: | Hiroaki Ikegami, Shingo Noguchi, Kazumasa Fukuda, Kentaro Akata, Kei Yamasaki, Toshinori Kawanami, Hiroshi Mukae, Kazuhiro Yatera |
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Format: | Article |
Language: | English |
Published: |
Nature Publishing Group
2021-10-01
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Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-021-98985-8 |
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