3D Printing PLA/Gingival Stem Cells/ EVs Upregulate miR-2861 and -210 during Osteoangiogenesis Commitment
Bone tissue regeneration strategies require approaches that provide an osteogenic and angiogenic microenvironment able to drive the bone growth. Recently, the development of 3D printing biomaterials, including poly(lactide) (3D-PLA), enriched with mesenchymal stem cells (MSCs) and/or their derivativ...
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doaj-b566f83ff34f4080ba317afa640a31262020-11-25T00:22:23ZengMDPI AGInternational Journal of Molecular Sciences1422-00672019-07-012013325610.3390/ijms20133256ijms201332563D Printing PLA/Gingival Stem Cells/ EVs Upregulate miR-2861 and -210 during Osteoangiogenesis CommitmentJacopo Pizzicannella0Francesca Diomede1Agnese Gugliandolo2Luigi Chiricosta3Placido Bramanti4Ilaria Merciaro5Tiziana Orsini6Emanuela Mazzon7Oriana Trubiani8Department of Medical, Oral and Biotechnological Sciences, University “G. d’Annunzio”, 66100 Chieti-Pescara, ItalyDepartment of Medical, Oral and Biotechnological Sciences, University “G. d’Annunzio”, 66100 Chieti-Pescara, ItalyIRCCS Centro Neurolesi “Bonino Pulejo”, 98124 Messina, ItalyIRCCS Centro Neurolesi “Bonino Pulejo”, 98124 Messina, ItalyIRCCS Centro Neurolesi “Bonino Pulejo”, 98124 Messina, ItalyDepartment of Medical, Oral and Biotechnological Sciences, University “G. d’Annunzio”, 66100 Chieti-Pescara, ItalyCNR-National Research Council, Institute of Cell Biology and Neurobiology (IBCN), Monterotondo, 00015 Roma, ItalyIRCCS Centro Neurolesi “Bonino Pulejo”, 98124 Messina, ItalyDepartment of Medical, Oral and Biotechnological Sciences, University “G. d’Annunzio”, 66100 Chieti-Pescara, ItalyBone tissue regeneration strategies require approaches that provide an osteogenic and angiogenic microenvironment able to drive the bone growth. Recently, the development of 3D printing biomaterials, including poly(lactide) (3D-PLA), enriched with mesenchymal stem cells (MSCs) and/or their derivatives, such as extracellular vesicles (EVs) has been achieving promising results. In this study, in vitro results showed an increased expression of osteogenic and angiogenic markers, as RUNX2, VEGFA, OPN and COL1A1 in the living construct 3D-PLA/human Gingival MSCs (hGMSCs)/EVs. Considering that EVs carry and transfer proteins, mRNA and microRNA into target cells, we evaluated miR-2861 and miR-210 expression related to osteoangiogenesis commitment. Histological examination of rats implanted with 3D-PLA/hGMSCs/EVs evidenced the activation of bone regeneration and of the vascularization process, confirmed also by MicroCT. In synthesis, an upregulation of miR-2861 and -210 other than RUNX2, VEGFA, OPN and COL1A1 was evident in cells cultured in the presence of the biomaterial and EVs. Then, these results evidenced that EVs may enhance bone regeneration in calvaria defects, in association with an enhanced vascularization offering a novel regulatory system in the osteoangiogenesis evolution. The application of new strategies to improve biomaterial engraftment is of great interest in the regenerative medicine and can represent a way to promote bone regeneration.https://www.mdpi.com/1422-0067/20/13/3256microRNAosteogenesisangiogenesismesenchymal stem cellsextracellular vesiclesscaffold |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jacopo Pizzicannella Francesca Diomede Agnese Gugliandolo Luigi Chiricosta Placido Bramanti Ilaria Merciaro Tiziana Orsini Emanuela Mazzon Oriana Trubiani |
spellingShingle |
Jacopo Pizzicannella Francesca Diomede Agnese Gugliandolo Luigi Chiricosta Placido Bramanti Ilaria Merciaro Tiziana Orsini Emanuela Mazzon Oriana Trubiani 3D Printing PLA/Gingival Stem Cells/ EVs Upregulate miR-2861 and -210 during Osteoangiogenesis Commitment International Journal of Molecular Sciences microRNA osteogenesis angiogenesis mesenchymal stem cells extracellular vesicles scaffold |
author_facet |
Jacopo Pizzicannella Francesca Diomede Agnese Gugliandolo Luigi Chiricosta Placido Bramanti Ilaria Merciaro Tiziana Orsini Emanuela Mazzon Oriana Trubiani |
author_sort |
Jacopo Pizzicannella |
title |
3D Printing PLA/Gingival Stem Cells/ EVs Upregulate miR-2861 and -210 during Osteoangiogenesis Commitment |
title_short |
3D Printing PLA/Gingival Stem Cells/ EVs Upregulate miR-2861 and -210 during Osteoangiogenesis Commitment |
title_full |
3D Printing PLA/Gingival Stem Cells/ EVs Upregulate miR-2861 and -210 during Osteoangiogenesis Commitment |
title_fullStr |
3D Printing PLA/Gingival Stem Cells/ EVs Upregulate miR-2861 and -210 during Osteoangiogenesis Commitment |
title_full_unstemmed |
3D Printing PLA/Gingival Stem Cells/ EVs Upregulate miR-2861 and -210 during Osteoangiogenesis Commitment |
title_sort |
3d printing pla/gingival stem cells/ evs upregulate mir-2861 and -210 during osteoangiogenesis commitment |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2019-07-01 |
description |
Bone tissue regeneration strategies require approaches that provide an osteogenic and angiogenic microenvironment able to drive the bone growth. Recently, the development of 3D printing biomaterials, including poly(lactide) (3D-PLA), enriched with mesenchymal stem cells (MSCs) and/or their derivatives, such as extracellular vesicles (EVs) has been achieving promising results. In this study, in vitro results showed an increased expression of osteogenic and angiogenic markers, as RUNX2, VEGFA, OPN and COL1A1 in the living construct 3D-PLA/human Gingival MSCs (hGMSCs)/EVs. Considering that EVs carry and transfer proteins, mRNA and microRNA into target cells, we evaluated miR-2861 and miR-210 expression related to osteoangiogenesis commitment. Histological examination of rats implanted with 3D-PLA/hGMSCs/EVs evidenced the activation of bone regeneration and of the vascularization process, confirmed also by MicroCT. In synthesis, an upregulation of miR-2861 and -210 other than RUNX2, VEGFA, OPN and COL1A1 was evident in cells cultured in the presence of the biomaterial and EVs. Then, these results evidenced that EVs may enhance bone regeneration in calvaria defects, in association with an enhanced vascularization offering a novel regulatory system in the osteoangiogenesis evolution. The application of new strategies to improve biomaterial engraftment is of great interest in the regenerative medicine and can represent a way to promote bone regeneration. |
topic |
microRNA osteogenesis angiogenesis mesenchymal stem cells extracellular vesicles scaffold |
url |
https://www.mdpi.com/1422-0067/20/13/3256 |
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