Comprehensive Profiling of Blood Coagulation and Fibrinolysis Marker Reveals Elevated Plasmin-Antiplasmin Complexes in Parkinson’s Disease
Parkinson’s disease (PD) is the second most common age-related neurodegenerative disease. Accumulating evidence demonstrates that alpha-synuclein (α-Syn), an apparently predominant neuronal protein, is a major contributor to PD pathology. As α-Syn is also highly abundant in blood, particularly in re...
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doaj-b56abce92eac4b839ec6b176420b17182021-08-26T13:32:03ZengMDPI AGBiology2079-77372021-07-011071671610.3390/biology10080716Comprehensive Profiling of Blood Coagulation and Fibrinolysis Marker Reveals Elevated Plasmin-Antiplasmin Complexes in Parkinson’s DiseaseAmit Sharma0Jens Müller1Karin Schuetze2Verena Rolfes3Rosi Bissinger4Nathalia Rosero5Ashar Ahmad6Bernardo S Franklin7Berndt Zur8Holger Fröhlich9Florian Lang10Johannes Oldenburg11Bernd Pötzsch12Ullrich Wüllner13Department of Neurology, University Hospital Bonn, 53127 Bonn, GermanyInstitute of Experimental Hematology and Transfusion Medicine, University Hospital Bonn, 53127 Bonn, GermanyCellTool Gmbh, Am Neuland 1, 82347 Bernried, GermanyInstitute of Innate Immunity, University Hospital Bonn, 53127 Bonn, GermanyDepartment of Internal Medicine IV, Eberhard Karl University, 72076 Tuebingen, GermanyInstitute of Innate Immunity, University Hospital Bonn, 53127 Bonn, GermanyBonn-Aachen International Center for IT (B-IT), University Hospital Bonn, 53115 Bonn, GermanyInstitute of Innate Immunity, University Hospital Bonn, 53127 Bonn, GermanyCentral Laboratory of the Rheinland Klinikum Neuss, 41464 Neuss, GermanyBonn-Aachen International Center for IT (B-IT), University Hospital Bonn, 53115 Bonn, GermanyDepartment of Physiology, Eberhard Karls University, 72076 Tuebingen, GermanyInstitute of Experimental Hematology and Transfusion Medicine, University Hospital Bonn, 53127 Bonn, GermanyInstitute of Experimental Hematology and Transfusion Medicine, University Hospital Bonn, 53127 Bonn, GermanyDepartment of Neurology, University Hospital Bonn, 53127 Bonn, GermanyParkinson’s disease (PD) is the second most common age-related neurodegenerative disease. Accumulating evidence demonstrates that alpha-synuclein (α-Syn), an apparently predominant neuronal protein, is a major contributor to PD pathology. As α-Syn is also highly abundant in blood, particularly in red blood cells (RBCs) and platelets, this in turn raises the question on the function of presumably dysfunctional α-Syn in “peripheral” cells and its putative effect on the other enclosed constituents. Herein, we detected the internal variance in erythrocytes of PD patients by Raman spectroscopy, but no measurable amount of erythrocytic behavioural change (eryptosis) or any haemoglobin variation was noticed. An elevated level of plasmin-antiplasmin complexes (PAP) was observed in the plasma of PD patients, indicating activation of the fibrinolytic system, but platelet activation after thrombin stimulation was not altered. Sex-specific patterns were noticed for blood coagulation factor XIII and factor XII activity in PD patients. Additionally, the alterations in homocysteine levels which have often been observed in PD patients were found to be independent from L-DOPA usage and PAP levels. Furthermore, a selective gene expression analysis identified subsets of genes related to different blood-associated compartments (RBCs, platelets, coagulation-fibrinolysis) also involved in PD-related pathways.https://www.mdpi.com/2079-7737/10/8/716Parkinson’s diseaseblood coagulationeryptosisplasmin-antiplasmin complexeshomocysteinehaemoglobin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Amit Sharma Jens Müller Karin Schuetze Verena Rolfes Rosi Bissinger Nathalia Rosero Ashar Ahmad Bernardo S Franklin Berndt Zur Holger Fröhlich Florian Lang Johannes Oldenburg Bernd Pötzsch Ullrich Wüllner |
spellingShingle |
Amit Sharma Jens Müller Karin Schuetze Verena Rolfes Rosi Bissinger Nathalia Rosero Ashar Ahmad Bernardo S Franklin Berndt Zur Holger Fröhlich Florian Lang Johannes Oldenburg Bernd Pötzsch Ullrich Wüllner Comprehensive Profiling of Blood Coagulation and Fibrinolysis Marker Reveals Elevated Plasmin-Antiplasmin Complexes in Parkinson’s Disease Biology Parkinson’s disease blood coagulation eryptosis plasmin-antiplasmin complexes homocysteine haemoglobin |
author_facet |
Amit Sharma Jens Müller Karin Schuetze Verena Rolfes Rosi Bissinger Nathalia Rosero Ashar Ahmad Bernardo S Franklin Berndt Zur Holger Fröhlich Florian Lang Johannes Oldenburg Bernd Pötzsch Ullrich Wüllner |
author_sort |
Amit Sharma |
title |
Comprehensive Profiling of Blood Coagulation and Fibrinolysis Marker Reveals Elevated Plasmin-Antiplasmin Complexes in Parkinson’s Disease |
title_short |
Comprehensive Profiling of Blood Coagulation and Fibrinolysis Marker Reveals Elevated Plasmin-Antiplasmin Complexes in Parkinson’s Disease |
title_full |
Comprehensive Profiling of Blood Coagulation and Fibrinolysis Marker Reveals Elevated Plasmin-Antiplasmin Complexes in Parkinson’s Disease |
title_fullStr |
Comprehensive Profiling of Blood Coagulation and Fibrinolysis Marker Reveals Elevated Plasmin-Antiplasmin Complexes in Parkinson’s Disease |
title_full_unstemmed |
Comprehensive Profiling of Blood Coagulation and Fibrinolysis Marker Reveals Elevated Plasmin-Antiplasmin Complexes in Parkinson’s Disease |
title_sort |
comprehensive profiling of blood coagulation and fibrinolysis marker reveals elevated plasmin-antiplasmin complexes in parkinson’s disease |
publisher |
MDPI AG |
series |
Biology |
issn |
2079-7737 |
publishDate |
2021-07-01 |
description |
Parkinson’s disease (PD) is the second most common age-related neurodegenerative disease. Accumulating evidence demonstrates that alpha-synuclein (α-Syn), an apparently predominant neuronal protein, is a major contributor to PD pathology. As α-Syn is also highly abundant in blood, particularly in red blood cells (RBCs) and platelets, this in turn raises the question on the function of presumably dysfunctional α-Syn in “peripheral” cells and its putative effect on the other enclosed constituents. Herein, we detected the internal variance in erythrocytes of PD patients by Raman spectroscopy, but no measurable amount of erythrocytic behavioural change (eryptosis) or any haemoglobin variation was noticed. An elevated level of plasmin-antiplasmin complexes (PAP) was observed in the plasma of PD patients, indicating activation of the fibrinolytic system, but platelet activation after thrombin stimulation was not altered. Sex-specific patterns were noticed for blood coagulation factor XIII and factor XII activity in PD patients. Additionally, the alterations in homocysteine levels which have often been observed in PD patients were found to be independent from L-DOPA usage and PAP levels. Furthermore, a selective gene expression analysis identified subsets of genes related to different blood-associated compartments (RBCs, platelets, coagulation-fibrinolysis) also involved in PD-related pathways. |
topic |
Parkinson’s disease blood coagulation eryptosis plasmin-antiplasmin complexes homocysteine haemoglobin |
url |
https://www.mdpi.com/2079-7737/10/8/716 |
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