Sex Steroid Metabolism in Benign and Malignant Intact Prostate Biopsies: Individual Profiling of Prostate Intracrinology
In vitro studies reveal that androgens, oestrogens, and their metabolites play a crucial role in prostate homeostasis. Most of the studies evaluated intraprostatic hormone metabolism using cell lines or preprocessed specimens. Using an ex vivo model of intact tissue cultures with preserved architect...
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Online Access: | http://dx.doi.org/10.1155/2014/464869 |
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doaj-b573989b27a545068afff55106c4e0f32020-11-25T00:47:08ZengHindawi LimitedBioMed Research International2314-61332314-61412014-01-01201410.1155/2014/464869464869Sex Steroid Metabolism in Benign and Malignant Intact Prostate Biopsies: Individual Profiling of Prostate IntracrinologyDaniele Gianfrilli0Silvia Pierotti1Riccardo Pofi2Costantino Leonardo3Mauro Ciccariello4Federica Barbagallo5Department of Experimental Medicine, Sapienza University, Viale del Policlinico 155A, 00161 Rome, ItalyDepartment of Experimental Medicine, Sapienza University, Viale del Policlinico 155A, 00161 Rome, ItalyDepartment of Experimental Medicine, Sapienza University, Viale del Policlinico 155A, 00161 Rome, ItalyDepartment of Urology, Sapienza University, 00161 Rome, ItalyDepartment of Radiology, Sapienza University, 00161 Rome, ItalyDepartment of Experimental Medicine, Sapienza University, Viale del Policlinico 155A, 00161 Rome, ItalyIn vitro studies reveal that androgens, oestrogens, and their metabolites play a crucial role in prostate homeostasis. Most of the studies evaluated intraprostatic hormone metabolism using cell lines or preprocessed specimens. Using an ex vivo model of intact tissue cultures with preserved architecture, we characterized the enzymatic profile of biopsies from patients with benign prostatic hyperplasia (BPH) or cancer (PC), focusing on 17β-hydroxy-steroid-dehydrogenases (17β-HSDs) and aromatase activities. Samples from 26 men who underwent prostate needle core biopsies (BPH n = 14; PC n = 12) were incubated with radiolabeled 3H-testosterone or 3H-androstenedione. Conversion was evaluated by TLC separation and beta-scanning of extracted supernatants. We identified three major patterns of conversion. The majority of BPHs revealed no active testosterone/oestradiol conversion as opposed to prostate cancer. Conversion correlated with histology and PSA, but not circulating hormones. Highest Gleason scores had a higher androstenedion-to-testosterone conversion and expression of 17β-HSD-isoenzymes-3/5. Conclusions. We developed an easy tool to profile individual intraprostatic enzymatic activity by characterizing conversion pathways in an intact tissue environment. In fresh biopsies we found that 17β-HSD-isoenzymes and aromatase activities correlate with biological behaviour allowing for morphofunctional phenotyping of pathology specimens and clinical monitoring of novel enzyme-targeting drugs.http://dx.doi.org/10.1155/2014/464869 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Daniele Gianfrilli Silvia Pierotti Riccardo Pofi Costantino Leonardo Mauro Ciccariello Federica Barbagallo |
spellingShingle |
Daniele Gianfrilli Silvia Pierotti Riccardo Pofi Costantino Leonardo Mauro Ciccariello Federica Barbagallo Sex Steroid Metabolism in Benign and Malignant Intact Prostate Biopsies: Individual Profiling of Prostate Intracrinology BioMed Research International |
author_facet |
Daniele Gianfrilli Silvia Pierotti Riccardo Pofi Costantino Leonardo Mauro Ciccariello Federica Barbagallo |
author_sort |
Daniele Gianfrilli |
title |
Sex Steroid Metabolism in Benign and Malignant Intact Prostate Biopsies: Individual Profiling of Prostate Intracrinology |
title_short |
Sex Steroid Metabolism in Benign and Malignant Intact Prostate Biopsies: Individual Profiling of Prostate Intracrinology |
title_full |
Sex Steroid Metabolism in Benign and Malignant Intact Prostate Biopsies: Individual Profiling of Prostate Intracrinology |
title_fullStr |
Sex Steroid Metabolism in Benign and Malignant Intact Prostate Biopsies: Individual Profiling of Prostate Intracrinology |
title_full_unstemmed |
Sex Steroid Metabolism in Benign and Malignant Intact Prostate Biopsies: Individual Profiling of Prostate Intracrinology |
title_sort |
sex steroid metabolism in benign and malignant intact prostate biopsies: individual profiling of prostate intracrinology |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2014-01-01 |
description |
In vitro studies reveal that androgens, oestrogens, and their metabolites play a crucial role in prostate homeostasis. Most of the studies evaluated intraprostatic hormone metabolism using cell lines or preprocessed specimens. Using an ex vivo model of intact tissue cultures with preserved architecture, we characterized the enzymatic profile of biopsies from patients with benign prostatic hyperplasia (BPH) or cancer (PC), focusing on 17β-hydroxy-steroid-dehydrogenases (17β-HSDs) and aromatase activities. Samples from 26 men who underwent prostate needle core biopsies (BPH n = 14; PC n = 12) were incubated with radiolabeled 3H-testosterone or 3H-androstenedione. Conversion was evaluated by TLC separation and beta-scanning of extracted supernatants. We identified three major patterns of conversion. The majority of BPHs revealed no active testosterone/oestradiol conversion as opposed to prostate cancer. Conversion correlated with histology and PSA, but not circulating hormones. Highest Gleason scores had a higher androstenedion-to-testosterone conversion and expression of 17β-HSD-isoenzymes-3/5. Conclusions. We developed an easy tool to profile individual intraprostatic enzymatic activity by characterizing conversion pathways in an intact tissue environment. In fresh biopsies we found that 17β-HSD-isoenzymes and aromatase activities correlate with biological behaviour allowing for morphofunctional phenotyping of pathology specimens and clinical monitoring of novel enzyme-targeting drugs. |
url |
http://dx.doi.org/10.1155/2014/464869 |
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