Frequency of regulatory T-cells in the peripheral blood of patients with pulmonary tuberculosis from shanxi province, china.
BACKGROUND: Tuberculosis (TB) is a disease caused by the chronic and continuous infection of the pathogen Mycobacterium tuberculosis (M. tuberculosis). M. tuberculosis is an intracellular bacterial pathogen and is eliminated mainly through CD4(+) effector Th cells. M. tuberculosis induces regulatory...
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doaj-b5745079601c4eb0ac1d20d5dc72af4f2020-11-25T02:08:42ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0186e6549610.1371/journal.pone.0065496Frequency of regulatory T-cells in the peripheral blood of patients with pulmonary tuberculosis from shanxi province, china.Hui PangQin YuBuping GuoYi JiangLi WanJun LiYanjun WuKanglin WanBACKGROUND: Tuberculosis (TB) is a disease caused by the chronic and continuous infection of the pathogen Mycobacterium tuberculosis (M. tuberculosis). M. tuberculosis is an intracellular bacterial pathogen and is eliminated mainly through CD4(+) effector Th cells. M. tuberculosis induces regulatory T lymphocytes (Tregs) that mediate immune suppression by cell-to-cell contact or by secreting cytokines such as transforming growth factor-β (TGF-β). To understand the role of regulatory T-cells in the pathogenesis of TB, we have measured the in vivo frequency of regulatory T-cells and associated in vivo cytokine production in pulmonary tuberculosis patients. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we analyzed blood samples from 3 different populations (Group 1: patients with active TB, Group 2: patients recovered from TB and Group 3: healthy controls). We measured natural regulatory T-cell expression in peripheral blood using flow cytometry, and levels of blood serum IFN-γ and TGF-β1 using ELISA. The in vivo function of inductive regulatory T cells was mainly indicated by the expression of IFN-γ, TGF-β1, etc. Frequencyof natural regulatory T cells and inductive regulatory T cells in the peripheral blood samples from Group 1 patients were all significantly higher (P<0.05) than those from Groups 2 and 3. CONCLUSION/SIGNIFICANCE: Our results indicate that frequency of natural regulatory T cells and inductive regulatory T cells are significantly higher in the peripheral blood of patients with active pulmonary tuberculosis. These findings have potential application in improving TB diagnostic methods.http://europepmc.org/articles/PMC3675031?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hui Pang Qin Yu Buping Guo Yi Jiang Li Wan Jun Li Yanjun Wu Kanglin Wan |
spellingShingle |
Hui Pang Qin Yu Buping Guo Yi Jiang Li Wan Jun Li Yanjun Wu Kanglin Wan Frequency of regulatory T-cells in the peripheral blood of patients with pulmonary tuberculosis from shanxi province, china. PLoS ONE |
author_facet |
Hui Pang Qin Yu Buping Guo Yi Jiang Li Wan Jun Li Yanjun Wu Kanglin Wan |
author_sort |
Hui Pang |
title |
Frequency of regulatory T-cells in the peripheral blood of patients with pulmonary tuberculosis from shanxi province, china. |
title_short |
Frequency of regulatory T-cells in the peripheral blood of patients with pulmonary tuberculosis from shanxi province, china. |
title_full |
Frequency of regulatory T-cells in the peripheral blood of patients with pulmonary tuberculosis from shanxi province, china. |
title_fullStr |
Frequency of regulatory T-cells in the peripheral blood of patients with pulmonary tuberculosis from shanxi province, china. |
title_full_unstemmed |
Frequency of regulatory T-cells in the peripheral blood of patients with pulmonary tuberculosis from shanxi province, china. |
title_sort |
frequency of regulatory t-cells in the peripheral blood of patients with pulmonary tuberculosis from shanxi province, china. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2013-01-01 |
description |
BACKGROUND: Tuberculosis (TB) is a disease caused by the chronic and continuous infection of the pathogen Mycobacterium tuberculosis (M. tuberculosis). M. tuberculosis is an intracellular bacterial pathogen and is eliminated mainly through CD4(+) effector Th cells. M. tuberculosis induces regulatory T lymphocytes (Tregs) that mediate immune suppression by cell-to-cell contact or by secreting cytokines such as transforming growth factor-β (TGF-β). To understand the role of regulatory T-cells in the pathogenesis of TB, we have measured the in vivo frequency of regulatory T-cells and associated in vivo cytokine production in pulmonary tuberculosis patients. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we analyzed blood samples from 3 different populations (Group 1: patients with active TB, Group 2: patients recovered from TB and Group 3: healthy controls). We measured natural regulatory T-cell expression in peripheral blood using flow cytometry, and levels of blood serum IFN-γ and TGF-β1 using ELISA. The in vivo function of inductive regulatory T cells was mainly indicated by the expression of IFN-γ, TGF-β1, etc. Frequencyof natural regulatory T cells and inductive regulatory T cells in the peripheral blood samples from Group 1 patients were all significantly higher (P<0.05) than those from Groups 2 and 3. CONCLUSION/SIGNIFICANCE: Our results indicate that frequency of natural regulatory T cells and inductive regulatory T cells are significantly higher in the peripheral blood of patients with active pulmonary tuberculosis. These findings have potential application in improving TB diagnostic methods. |
url |
http://europepmc.org/articles/PMC3675031?pdf=render |
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