Morphological Responses and Gene Expression of Grain Amaranth (<i>Amaranthus</i> spp.) Growing under Cd

• Main Authors: Veronika Lancíková, Marián Tomka, Jana Žiarovská, Ján Gažo, Andrea Hricová
• Format: Article
• Language: English
• Published: MDPI AG 2020-04-01
• Series: Plants
• Subjects: cadmium stress; grain amaranth; stress-related genes; phytoremediation potential
• Online Access: https://www.mdpi.com/2223-7747/9/5/572

<b> </b>Phytoremediation<b> </b>efficiency depends on the ability of plants to accumulate, translocate and resist high levels of metals without symptoms of toxicity. This study was conducted to evaluate the potential of grain amaranth for remediation of soils contaminated with Cd. Three grain amaranth varieties, “Pribina” (<i>A. </i><i>cruentus</i>), “Zobor” (<i>A. </i><i>hypochondriacus</i><i> x A. </i><i>hybridus</i>) and Plainsman (<i>A. </i><i>hypochondriacus</i><i> x A. </i><i>hybridus</i>) were tested under different level of Cd (0, 5, 10 and 15 mg/L) in a hydroponic experimental treatment. All could be classified as Cd excluders or Cd-hypertolerant varieties able to grow and accumulate significant amounts of Cd from the hydroponic solution, preferentially in the roots. Under the highest level of Cd exposure, qRT-PCR expression analysis of five stress-related genes was examined in above- and below-ground biomass. The results show that the Cd concentration significantly increased the mRNA level of chitinase 5 (<i>Chit 5)</i> in amaranth roots as the primary site of metal stress. The involvement of phytochelatin synthase (<i>PCS1</i>) in Cd detoxification is suggested. Based on our findings, we can conclude that variety “Pribina” is the most Cd-tolerant among three tested and can be expected to be used in the phytomanagement of Cd loaded soils as an effective phytostabiliser.