T cell responses against tumor associated antigens and prognosis in colorectal cancer patients

• Main Authors: Rivoltini Licia, Hegewisch-Becker Susanna, Buhr Heinz-Johannes, Germer Christoph-Thomas, Letsch Anne, Scheibenbogen Carmen, Nagorsen Dirk, Thiel Eckhard, Keilholz Ulrich
• Format: Article
• Language: English
• Published: BMC 2005-01-01
• Series: Journal of Translational Medicine
• Subjects: T cell response; antigen; colorectal cancer; survival; prognosis
• Online Access: http://www.translational-medicine.com/content/3/1/3

<p>Abstract</p> <p>Introduction</p> <p>Spontaneous T cell responses against specific tumor-associated antigens (TAA) are frequently detected in peripheral blood of tumor patients of various histiotypes. However, little is known about whether these circulating, spontaneously occurring, TAA-reactive T cells influence the clinical course of disease.</p> <p>Methods</p> <p>Fifty-four HLA-A2 positive colorectal cancer patients had been analyzed for the presence of T cell responses against epitopes derived from the TAA Ep-CAM, her-2/<it>neu</it>, and CEA either by ELISPOT assay or by intracellular cytokine staining. Then, Kaplan-Meier survival analysis was performed comparing T-cell-responders and T-cell-non-responders. For comparison, a group of T-cell-non-responders was compiled stringently matched to T-cell-responders based on clinical criteria and also analyzed for survival.</p> <p>Results</p> <p>Sixteen out of 54 patients had a detectable T cell response against at least one of the three tested TAA. Two out of 21 patients (9.5%) with limited stage of disease (UICC I and II) and 14 out of 33 patients (42.4%) with advanced disease (UICC III and IV) were T cell response positive. Comparing all T-cell-responders (n = 16) and all T-cell-non-responders (n = 38), no survival difference was found. In an attempt to reduce the influence of confounding clinical factors, we then compared 16 responders and 16 non-responders in a matched group survival analysis; and again no survival difference was found (p = 0.7).</p> <p>Conclusion</p> <p>In summary, we found no evidence that spontaneous peripheral T cell responses against HLA-A2-binding epitopes of CEA, her-2/neu and Ep-CAM are a strong prognostic factor for survival.</p>