Genome-wide hypomethylation of LINE-1 and Alu retroelements in cell-free DNA of blood is an epigenetic biomarker of human aging
Aging associated DNA hypomethylation of LINE-1 and Alu retroelements may be a crucial determinant of loss of genomic integrity, deterioration and cancer. In peripheral blood LINE-1 hypomethylation has been reported to increase during aging, but other studies did not observe significant changes. We h...
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doaj-b58f61be5f3340bab1f7d344169da70c2020-11-25T00:26:36ZengElsevierSaudi Journal of Biological Sciences1319-562X2018-09-0125612201226Genome-wide hypomethylation of LINE-1 and Alu retroelements in cell-free DNA of blood is an epigenetic biomarker of human agingLars Erichsen0Agnes Beermann1Marcos J. Arauzo-Bravo2Mohamed Hassan3Mohamed A. Dkhil4Saleh Al-Quraishy5Taghreed A. Hafiz6Johannes C. Fischer7Simeon Santourlidis8Epigenetics Core Laboratory, Institute of Transplantation Diagnostics and Cell Therapeutics, Medical Faculty, Heinrich-Heine University Duesseldorf, Moorenstr. 5, 40225 Duesseldorf, GermanyEpigenetics Core Laboratory, Institute of Transplantation Diagnostics and Cell Therapeutics, Medical Faculty, Heinrich-Heine University Duesseldorf, Moorenstr. 5, 40225 Duesseldorf, GermanyGroup of Computational Biology and Systems Biomedicine, Biodonostia Health Research Institute, 20014 San Sebastián, Spain; IKERBASQUE, Basque Foundation for Science, 48009 Bilbao, SpainDepartment of Surgery, Tulane University School of Medicine, New Orleans, LA 70112, USA; Institut National de la Santé et de la Recherche Médicale, University of Strasbourg, 67000 Strasbourg, FranceDepartment of Zoology, College of Science, King Saud University, Saudi Arabia; Department of Zoology and Entomology, Faculty of Science, Helwan University, Cairo, EgyptDepartment of Zoology, College of Science, King Saud University, Saudi ArabiaClinical Laboratory Sciences Department, College of Applied Medical Sciences, King Saud University, Saudi ArabiaInstitute of Transplantation Diagnostics and Cell Therapeutics, University Hospital and Medical Faculty of the Heinrich-Heine-University Duesseldorf, Moorenstr. 5, Duesseldorf 40225, GermanyEpigenetics Core Laboratory, Institute of Transplantation Diagnostics and Cell Therapeutics, Medical Faculty, Heinrich-Heine University Duesseldorf, Moorenstr. 5, 40225 Duesseldorf, Germany; Corresponding author.Aging associated DNA hypomethylation of LINE-1 and Alu retroelements may be a crucial determinant of loss of genomic integrity, deterioration and cancer. In peripheral blood LINE-1 hypomethylation has been reported to increase during aging, but other studies did not observe significant changes. We hypothesized that these apparently inconsistent reports might relate to differences between cellular and cell-free DNA. Using the technique of idiolocal normalization of real-time methylation-specific PCR (IDLN-MSP) for genetic imbalanced DNA specimens we obtained evidence that LINE-1 hypomethylation in cell-free DNA, but not cellular DNA from peripheral blood is an epigenetic biomarker for human aging. Furthermore, hypomethylation of cell-free DNA is more extensive in smokers, suggesting that it might be used as a surrogate marker for monitoring the improvement of smoking-induced adverse effects after cancelling smoking. Keywords: Epigenetics, DNA methylation, Cell-free DNA, Retroelements, LINE-1http://www.sciencedirect.com/science/article/pii/S1319562X18300408 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lars Erichsen Agnes Beermann Marcos J. Arauzo-Bravo Mohamed Hassan Mohamed A. Dkhil Saleh Al-Quraishy Taghreed A. Hafiz Johannes C. Fischer Simeon Santourlidis |
spellingShingle |
Lars Erichsen Agnes Beermann Marcos J. Arauzo-Bravo Mohamed Hassan Mohamed A. Dkhil Saleh Al-Quraishy Taghreed A. Hafiz Johannes C. Fischer Simeon Santourlidis Genome-wide hypomethylation of LINE-1 and Alu retroelements in cell-free DNA of blood is an epigenetic biomarker of human aging Saudi Journal of Biological Sciences |
author_facet |
Lars Erichsen Agnes Beermann Marcos J. Arauzo-Bravo Mohamed Hassan Mohamed A. Dkhil Saleh Al-Quraishy Taghreed A. Hafiz Johannes C. Fischer Simeon Santourlidis |
author_sort |
Lars Erichsen |
title |
Genome-wide hypomethylation of LINE-1 and Alu retroelements in cell-free DNA of blood is an epigenetic biomarker of human aging |
title_short |
Genome-wide hypomethylation of LINE-1 and Alu retroelements in cell-free DNA of blood is an epigenetic biomarker of human aging |
title_full |
Genome-wide hypomethylation of LINE-1 and Alu retroelements in cell-free DNA of blood is an epigenetic biomarker of human aging |
title_fullStr |
Genome-wide hypomethylation of LINE-1 and Alu retroelements in cell-free DNA of blood is an epigenetic biomarker of human aging |
title_full_unstemmed |
Genome-wide hypomethylation of LINE-1 and Alu retroelements in cell-free DNA of blood is an epigenetic biomarker of human aging |
title_sort |
genome-wide hypomethylation of line-1 and alu retroelements in cell-free dna of blood is an epigenetic biomarker of human aging |
publisher |
Elsevier |
series |
Saudi Journal of Biological Sciences |
issn |
1319-562X |
publishDate |
2018-09-01 |
description |
Aging associated DNA hypomethylation of LINE-1 and Alu retroelements may be a crucial determinant of loss of genomic integrity, deterioration and cancer. In peripheral blood LINE-1 hypomethylation has been reported to increase during aging, but other studies did not observe significant changes. We hypothesized that these apparently inconsistent reports might relate to differences between cellular and cell-free DNA. Using the technique of idiolocal normalization of real-time methylation-specific PCR (IDLN-MSP) for genetic imbalanced DNA specimens we obtained evidence that LINE-1 hypomethylation in cell-free DNA, but not cellular DNA from peripheral blood is an epigenetic biomarker for human aging. Furthermore, hypomethylation of cell-free DNA is more extensive in smokers, suggesting that it might be used as a surrogate marker for monitoring the improvement of smoking-induced adverse effects after cancelling smoking. Keywords: Epigenetics, DNA methylation, Cell-free DNA, Retroelements, LINE-1 |
url |
http://www.sciencedirect.com/science/article/pii/S1319562X18300408 |
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