Effect of Pentacyclic Triterpenoids-Rich Callus Extract of <i>Chaenomeles japonica</i> (Thunb.) Lindl. ex Spach on Viability, Morphology, and Proliferation of Normal Human Skin Fibroblasts
The effect of the well-characterized callus extract of <i>Chaenomeles japonica</i> on viability, morphology, and proliferation of normal human skin fibroblasts was investigated. The phytochemical analysis was performed using the ultra-high performance liquid chromatography method. The to...
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doaj-b59a5d7ff47f43e586da17875ec62ebf2020-11-24T22:37:33ZengMDPI AGMolecules1420-30492018-11-012311300910.3390/molecules23113009molecules23113009Effect of Pentacyclic Triterpenoids-Rich Callus Extract of <i>Chaenomeles japonica</i> (Thunb.) Lindl. ex Spach on Viability, Morphology, and Proliferation of Normal Human Skin FibroblastsMałgorzata Anna Kikowska0Małgorzata Chmielewska1Agata Włodarczyk2Elżbieta Studzińska-Sroka3Jerzy Żuchowski4Anna Stochmal5Małgorzata Kotwicka6Barbara Thiem7Department of Pharmaceutical Botany and Plant Biotechnology, Poznan University of Medical Sciences, 61-861 Poznań, PolandDepartment of Cell Biology, Poznan University of Medical Sciences, 60-806 Poznań, PolandDepartment of Pharmaceutical Botany and Plant Biotechnology, Poznan University of Medical Sciences, 61-861 Poznań, PolandDepartment of Pharmacognosy, Poznan University of Medical Sciences, 60-781 Poznań, PolandDepartment of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation, State Research Institute, 24-100 Puławy, PolandDepartment of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation, State Research Institute, 24-100 Puławy, PolandDepartment of Cell Biology, Poznan University of Medical Sciences, 60-806 Poznań, PolandDepartment of Pharmaceutical Botany and Plant Biotechnology, Poznan University of Medical Sciences, 61-861 Poznań, PolandThe effect of the well-characterized callus extract of <i>Chaenomeles japonica</i> on viability, morphology, and proliferation of normal human skin fibroblasts was investigated. The phytochemical analysis was performed using the ultra-high performance liquid chromatography method. The total phenolic, phenolic acid, and flavonoid contents were determined spectrophotometrically. The antioxidant activity was investigated using the DPPH (1,1-Diphenyl-1-picrylhydrazyl Radical Scavenging), FRAP (Ferric Reducing Antioxidant Power), and CUPRAC (CUPric Reducing Antioxidant Capacity) assays. The callus growth index during passages was high as well as the content of pentacyclic triterpenoids. The microscopic observations of the fibroblast viability, morphology and the evaluation of the proliferation ratio (xCELLigence system) proved that the influence of callus extract on the fibroblasts was dose-dependent. The evaluated level of fibroblasts proliferation rate after 72 h of incubation with callus extract at concentration 12.5 µg L<sup>−1</sup> was the highest compared to all the analyzed ligands. Moreover, callus extract administrated for 72 h caused a significant increase in the proliferation rate in comparison with the control group (5.7 ± 0.1 vs. 4.4 ± 0.9; <i>p</i> < 0.01). The preliminary studies carried out may suggest that the callus extract rich in triterpenoids may be a potential source of cosmetic ingredients with a beneficial effect on human skin.https://www.mdpi.com/1420-3049/23/11/3009<i>Chaenomeles japonica</i>polyphenolicspentacyclic triterpenesantioxidant activityin vitro culturesskin fibroblasts |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Małgorzata Anna Kikowska Małgorzata Chmielewska Agata Włodarczyk Elżbieta Studzińska-Sroka Jerzy Żuchowski Anna Stochmal Małgorzata Kotwicka Barbara Thiem |
spellingShingle |
Małgorzata Anna Kikowska Małgorzata Chmielewska Agata Włodarczyk Elżbieta Studzińska-Sroka Jerzy Żuchowski Anna Stochmal Małgorzata Kotwicka Barbara Thiem Effect of Pentacyclic Triterpenoids-Rich Callus Extract of <i>Chaenomeles japonica</i> (Thunb.) Lindl. ex Spach on Viability, Morphology, and Proliferation of Normal Human Skin Fibroblasts Molecules <i>Chaenomeles japonica</i> polyphenolics pentacyclic triterpenes antioxidant activity in vitro cultures skin fibroblasts |
author_facet |
Małgorzata Anna Kikowska Małgorzata Chmielewska Agata Włodarczyk Elżbieta Studzińska-Sroka Jerzy Żuchowski Anna Stochmal Małgorzata Kotwicka Barbara Thiem |
author_sort |
Małgorzata Anna Kikowska |
title |
Effect of Pentacyclic Triterpenoids-Rich Callus Extract of <i>Chaenomeles japonica</i> (Thunb.) Lindl. ex Spach on Viability, Morphology, and Proliferation of Normal Human Skin Fibroblasts |
title_short |
Effect of Pentacyclic Triterpenoids-Rich Callus Extract of <i>Chaenomeles japonica</i> (Thunb.) Lindl. ex Spach on Viability, Morphology, and Proliferation of Normal Human Skin Fibroblasts |
title_full |
Effect of Pentacyclic Triterpenoids-Rich Callus Extract of <i>Chaenomeles japonica</i> (Thunb.) Lindl. ex Spach on Viability, Morphology, and Proliferation of Normal Human Skin Fibroblasts |
title_fullStr |
Effect of Pentacyclic Triterpenoids-Rich Callus Extract of <i>Chaenomeles japonica</i> (Thunb.) Lindl. ex Spach on Viability, Morphology, and Proliferation of Normal Human Skin Fibroblasts |
title_full_unstemmed |
Effect of Pentacyclic Triterpenoids-Rich Callus Extract of <i>Chaenomeles japonica</i> (Thunb.) Lindl. ex Spach on Viability, Morphology, and Proliferation of Normal Human Skin Fibroblasts |
title_sort |
effect of pentacyclic triterpenoids-rich callus extract of <i>chaenomeles japonica</i> (thunb.) lindl. ex spach on viability, morphology, and proliferation of normal human skin fibroblasts |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2018-11-01 |
description |
The effect of the well-characterized callus extract of <i>Chaenomeles japonica</i> on viability, morphology, and proliferation of normal human skin fibroblasts was investigated. The phytochemical analysis was performed using the ultra-high performance liquid chromatography method. The total phenolic, phenolic acid, and flavonoid contents were determined spectrophotometrically. The antioxidant activity was investigated using the DPPH (1,1-Diphenyl-1-picrylhydrazyl Radical Scavenging), FRAP (Ferric Reducing Antioxidant Power), and CUPRAC (CUPric Reducing Antioxidant Capacity) assays. The callus growth index during passages was high as well as the content of pentacyclic triterpenoids. The microscopic observations of the fibroblast viability, morphology and the evaluation of the proliferation ratio (xCELLigence system) proved that the influence of callus extract on the fibroblasts was dose-dependent. The evaluated level of fibroblasts proliferation rate after 72 h of incubation with callus extract at concentration 12.5 µg L<sup>−1</sup> was the highest compared to all the analyzed ligands. Moreover, callus extract administrated for 72 h caused a significant increase in the proliferation rate in comparison with the control group (5.7 ± 0.1 vs. 4.4 ± 0.9; <i>p</i> < 0.01). The preliminary studies carried out may suggest that the callus extract rich in triterpenoids may be a potential source of cosmetic ingredients with a beneficial effect on human skin. |
topic |
<i>Chaenomeles japonica</i> polyphenolics pentacyclic triterpenes antioxidant activity in vitro cultures skin fibroblasts |
url |
https://www.mdpi.com/1420-3049/23/11/3009 |
work_keys_str_mv |
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