COMPARATIVE IN SILICO ANALYSIS OF ATP1A3 GENE IN MAMMALS

• Main Authors: Chinmoy Mishra, Siddhant Sekhar Sahoo, Sidharth Prasad Mishra, Stuti Tanaya Mohanty, Gangadhar Nayak, Kumaresh Behera, Kamdev Sethy
• Format: Article
• Language: English
• Published: West Bengal Veterinary Alumni Association 2018-06-01
• Series: Exploratory Animal and Medical Research
• Subjects: ATP1A3; Bioinformatics; Docking; Gene; STRING
• Online Access: http://www.animalmedicalresearch.org/Vol.8_Issue-1_June_2018/COMPARATIVE%20IN%20SILICO.pdf

Progress in the field of bioinformatics is useful to understand the global network of genes and their protein products. Genetic variants of the ATP1A3 gene have been suggested to be involved in the salt hypertension and feed intake, all of which may evidently affect heat production, heat loss and water mineral balance. In the present study, comparative analysis of ATP1A3 (ATPase Na+/K+ Transporting Subunit Alpha 3) proteins of different livestock species were carried out using bioinformatics tools. The results of this study indicate that most of physico-chemical properties were almost same in pig and cat. The global network analysis of ATP1A3 gene by, STRING 10 tool speculated its interaction with several other proteins like - ATP1A1, ATP1A2, ATP1A4, ATP1B1, ATP1B3, FXYD2 in different livestock species having high confidence score. The strong interaction was seen between ATP1B1 and ATP1A3 with a high score in pig. Molecular docking between ATP1A3 and ATP1B1 of pig has been carried out. Conserved region was present in all the nine studied different livestock species protein sequences despite speciation due to evolution. The present study will further support to understand the role of associated proteins in various cellular pathways in cattle. This work is also useful for the study of structural and functional analysis of ATP1A3 protein.