Determination of repaglinide in human plasma by high-performance liquid chromatography–tandem mass spectrometry
A rapid and sensitive method based on high-performance liquid chromatography–tandem mass spectrometry (LC–MS/MS) has been developed for the determination of repaglinide in human plasma. The analyte and internal standard (I.S.), diazepam, were extracted from plasma (25 μL) by liquid–liquid extraction...
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2011-06-01
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doaj-b5f12487cbea47e683b5bd505321ed8d2020-11-24T22:32:25ZengElsevierActa Pharmaceutica Sinica B2211-38352211-38432011-06-0111404510.1016/j.apsb.2011.04.004Determination of repaglinide in human plasma by high-performance liquid chromatography–tandem mass spectrometryJie Zhang0Feng Gao1Xin Guan2Yan-tong Sun3Jing-kai Gu4J. Paul Fawcett5Research Center for Drug Metabolism, College of Life Science, Jilin University, Changchun 130012, People's Republic of ChinaResearch Center for Drug Metabolism, College of Life Science, Jilin University, Changchun 130012, People's Republic of ChinaResearch Center for Drug Metabolism, College of Life Science, Jilin University, Changchun 130012, People's Republic of ChinaResearch Center for Drug Metabolism, College of Life Science, Jilin University, Changchun 130012, People's Republic of ChinaResearch Center for Drug Metabolism, College of Life Science, Jilin University, Changchun 130012, People's Republic of ChinaSchool of Pharmacy, University of Otago, Dunedin, New ZealandA rapid and sensitive method based on high-performance liquid chromatography–tandem mass spectrometry (LC–MS/MS) has been developed for the determination of repaglinide in human plasma. The analyte and internal standard (I.S.), diazepam, were extracted from plasma (25 μL) by liquid–liquid extraction with diethyl ether–dichloromethane (60:40, v/v) and separated on a XDB-C18 column using acetonitrile–ammonium acetate buffer (pH 6.8, 0.01 mol/L) as mobile phase. The retention times of repaglinide and I.S. were 1.95 and 2.35 min, respectively. Detection was carried out using API 4000 mass spectrometer with an ESI interface operating in the multiple reaction monitoring (MRM) mode. The assay was linear over the concentration range 0.050–50 ng/mL with a limit of detection (LOD) of 0.010 ng/mL. Intra- and inter-day precisions (as relative standard deviation, R.S.D.) were ≤5.07% and ≤11.2%, respectively, and accuracy (as relative error, R.E.) was from −0.593% to −1.26%. The assay was successfully applied to a pharmacokinetic study involving a single oral administration of a tablet containing 2 mg repaglinide to each of 10 healthy volunteers.http://www.sciencedirect.com/science/article/pii/S2211383511000050RepaglinidePharmacokineticsLC–MS/MSHuman plasma |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jie Zhang Feng Gao Xin Guan Yan-tong Sun Jing-kai Gu J. Paul Fawcett |
spellingShingle |
Jie Zhang Feng Gao Xin Guan Yan-tong Sun Jing-kai Gu J. Paul Fawcett Determination of repaglinide in human plasma by high-performance liquid chromatography–tandem mass spectrometry Acta Pharmaceutica Sinica B Repaglinide Pharmacokinetics LC–MS/MS Human plasma |
author_facet |
Jie Zhang Feng Gao Xin Guan Yan-tong Sun Jing-kai Gu J. Paul Fawcett |
author_sort |
Jie Zhang |
title |
Determination of repaglinide in human plasma by high-performance liquid chromatography–tandem mass spectrometry |
title_short |
Determination of repaglinide in human plasma by high-performance liquid chromatography–tandem mass spectrometry |
title_full |
Determination of repaglinide in human plasma by high-performance liquid chromatography–tandem mass spectrometry |
title_fullStr |
Determination of repaglinide in human plasma by high-performance liquid chromatography–tandem mass spectrometry |
title_full_unstemmed |
Determination of repaglinide in human plasma by high-performance liquid chromatography–tandem mass spectrometry |
title_sort |
determination of repaglinide in human plasma by high-performance liquid chromatography–tandem mass spectrometry |
publisher |
Elsevier |
series |
Acta Pharmaceutica Sinica B |
issn |
2211-3835 2211-3843 |
publishDate |
2011-06-01 |
description |
A rapid and sensitive method based on high-performance liquid chromatography–tandem mass spectrometry (LC–MS/MS) has been developed for the determination of repaglinide in human plasma. The analyte and internal standard (I.S.), diazepam, were extracted from plasma (25 μL) by liquid–liquid extraction with diethyl ether–dichloromethane (60:40, v/v) and separated on a XDB-C18 column using acetonitrile–ammonium acetate buffer (pH 6.8, 0.01 mol/L) as mobile phase. The retention times of repaglinide and I.S. were 1.95 and 2.35 min, respectively. Detection was carried out using API 4000 mass spectrometer with an ESI interface operating in the multiple reaction monitoring (MRM) mode. The assay was linear over the concentration range 0.050–50 ng/mL with a limit of detection (LOD) of 0.010 ng/mL. Intra- and inter-day precisions (as relative standard deviation, R.S.D.) were ≤5.07% and ≤11.2%, respectively, and accuracy (as relative error, R.E.) was from −0.593% to −1.26%. The assay was successfully applied to a pharmacokinetic study involving a single oral administration of a tablet containing 2 mg repaglinide to each of 10 healthy volunteers. |
topic |
Repaglinide Pharmacokinetics LC–MS/MS Human plasma |
url |
http://www.sciencedirect.com/science/article/pii/S2211383511000050 |
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