Proteomic and mechanistic study of Qingxuan Tongluo formula and curcumin in the treatment of Mycoplasma pneumoniae pneumonia
Objective: Mycoplasma pneumoniae (MP) is the only pathogen in the Mycoplasma family that can cause respiratory symptoms, including acute upper respiratory tract infection and bronchitis, which are often attributed to Mycoplasma pneumoniae pneumonia (MPP). MPP is one of the diseases that commonly aff...
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doaj-b5f646d1d2fe488398255833b11b34c92021-05-21T04:19:10ZengElsevierBiomedicine & Pharmacotherapy0753-33222021-01-01133110998Proteomic and mechanistic study of Qingxuan Tongluo formula and curcumin in the treatment of Mycoplasma pneumoniae pneumoniaLina Wei0Weilong Zhong1Tao Sun2Huanmin Li3Ting Sun4Yaowei Han5Dan Sun6Xinmin Li7First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China; First Affiliated Hospital to Changchun University of Chinese Medicine, Changchun Jilin 130021, ChinaNankai University, Tianjin 300071, China; Tianjin Medical University General Hospital, Tianjin 300052, ChinaFirst Affiliated Hospital to Changchun University of Chinese Medicine, Changchun Jilin 130021, ChinaFirst Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, ChinaFirst Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China; Beijing University of Chinese Medicine Affiliated Dongzhimen Hospital, Beijing 100007, ChinaFirst Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, ChinaFirst Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, ChinaFirst Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China; Corresponding author.Objective: Mycoplasma pneumoniae (MP) is the only pathogen in the Mycoplasma family that can cause respiratory symptoms, including acute upper respiratory tract infection and bronchitis, which are often attributed to Mycoplasma pneumoniae pneumonia (MPP). MPP is one of the diseases that commonly affects the pediatric respiratory system, but its pathogenesis is unclear. This study investigated the therapeutic effects and mechanisms of Qingxuan Tongluo formula and its main component, curcumin, on MPP. Methods: A mouse model of MPP was obtained by nasal drip of the MP strain. The effects of Qingxuan Tongluo formula and curcumin on the treatment of MPP were studied. The proteomic profiles of the alveolar lavage fluid of mice in the model group, Qingxuan Tongluo formula group and curcumin group were evaluated by LC–MS/MS. ELISA and immunohistochemistry were used to verify the possible presence of MP infection biomarkers and drug target proteins. Results: Compared with the mice in the model group, the MPP mice in the Qingxuan Tongluo formula group had significantly reduced fever and cough and prolonged the cough incubation period. Moreover, the pulmonary pathology of the MPP mice was significantly improved, and the lung histopathological score was decreased. After treatment with Qingxuan Tongluo formula and curcumin, the functional and pathway abnormalities caused by MP were mainly inhibited. Levels of HSP90AA1, GRP94, ENO1 and PLG expression were verified by ELISA and immunohistochemistry. Conclusion: Qingxuan Tongluo formula significantly reduced fevers and cough and prolonged the cough incubation period of MPP mice. Qingxuan Tongluo formula and curcumin significantly improved the pathological changes in lung tissue caused by MP infection. Proteomics analyses indicated that Qingxuan Tongluo formula and curcumin may have therapeutic effects on MPP by regulating energy metabolism, relieving oxidative stress and activating the fibrinolytic system. ENO1 and PLG were found to be potential drug targets.http://www.sciencedirect.com/science/article/pii/S0753332220311902Qingxuan Tongluo formulaCurcuminMPPProteomicsDrug target |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lina Wei Weilong Zhong Tao Sun Huanmin Li Ting Sun Yaowei Han Dan Sun Xinmin Li |
spellingShingle |
Lina Wei Weilong Zhong Tao Sun Huanmin Li Ting Sun Yaowei Han Dan Sun Xinmin Li Proteomic and mechanistic study of Qingxuan Tongluo formula and curcumin in the treatment of Mycoplasma pneumoniae pneumonia Biomedicine & Pharmacotherapy Qingxuan Tongluo formula Curcumin MPP Proteomics Drug target |
author_facet |
Lina Wei Weilong Zhong Tao Sun Huanmin Li Ting Sun Yaowei Han Dan Sun Xinmin Li |
author_sort |
Lina Wei |
title |
Proteomic and mechanistic study of Qingxuan Tongluo formula and curcumin in the treatment of Mycoplasma pneumoniae pneumonia |
title_short |
Proteomic and mechanistic study of Qingxuan Tongluo formula and curcumin in the treatment of Mycoplasma pneumoniae pneumonia |
title_full |
Proteomic and mechanistic study of Qingxuan Tongluo formula and curcumin in the treatment of Mycoplasma pneumoniae pneumonia |
title_fullStr |
Proteomic and mechanistic study of Qingxuan Tongluo formula and curcumin in the treatment of Mycoplasma pneumoniae pneumonia |
title_full_unstemmed |
Proteomic and mechanistic study of Qingxuan Tongluo formula and curcumin in the treatment of Mycoplasma pneumoniae pneumonia |
title_sort |
proteomic and mechanistic study of qingxuan tongluo formula and curcumin in the treatment of mycoplasma pneumoniae pneumonia |
publisher |
Elsevier |
series |
Biomedicine & Pharmacotherapy |
issn |
0753-3322 |
publishDate |
2021-01-01 |
description |
Objective: Mycoplasma pneumoniae (MP) is the only pathogen in the Mycoplasma family that can cause respiratory symptoms, including acute upper respiratory tract infection and bronchitis, which are often attributed to Mycoplasma pneumoniae pneumonia (MPP). MPP is one of the diseases that commonly affects the pediatric respiratory system, but its pathogenesis is unclear. This study investigated the therapeutic effects and mechanisms of Qingxuan Tongluo formula and its main component, curcumin, on MPP. Methods: A mouse model of MPP was obtained by nasal drip of the MP strain. The effects of Qingxuan Tongluo formula and curcumin on the treatment of MPP were studied. The proteomic profiles of the alveolar lavage fluid of mice in the model group, Qingxuan Tongluo formula group and curcumin group were evaluated by LC–MS/MS. ELISA and immunohistochemistry were used to verify the possible presence of MP infection biomarkers and drug target proteins. Results: Compared with the mice in the model group, the MPP mice in the Qingxuan Tongluo formula group had significantly reduced fever and cough and prolonged the cough incubation period. Moreover, the pulmonary pathology of the MPP mice was significantly improved, and the lung histopathological score was decreased. After treatment with Qingxuan Tongluo formula and curcumin, the functional and pathway abnormalities caused by MP were mainly inhibited. Levels of HSP90AA1, GRP94, ENO1 and PLG expression were verified by ELISA and immunohistochemistry. Conclusion: Qingxuan Tongluo formula significantly reduced fevers and cough and prolonged the cough incubation period of MPP mice. Qingxuan Tongluo formula and curcumin significantly improved the pathological changes in lung tissue caused by MP infection. Proteomics analyses indicated that Qingxuan Tongluo formula and curcumin may have therapeutic effects on MPP by regulating energy metabolism, relieving oxidative stress and activating the fibrinolytic system. ENO1 and PLG were found to be potential drug targets. |
topic |
Qingxuan Tongluo formula Curcumin MPP Proteomics Drug target |
url |
http://www.sciencedirect.com/science/article/pii/S0753332220311902 |
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