Electrochemical treatment: An investigation of dose-response relationships using an isolated liver perfusion model
Background/Aim: Ablative techniques such as radiofrequency ablation or non-thermal electrochemical treatment (ECT) are used to manage unresectable liver metastases. Although ECT is not affected by the cooling effect from adjacent vessels, there is a paucity of data available on ECT. Materials and Me...
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Wolters Kluwer Medknow Publications
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doaj-b5f9f330e52f44ddbf3ba63afa07ab122020-11-25T01:57:12ZengWolters Kluwer Medknow PublicationsThe Saudi Journal of Gastroenterology1319-37671998-40492011-01-0117533534210.4103/1319-3767.84491Electrochemical treatment: An investigation of dose-response relationships using an isolated liver perfusion modelRalf CzymekDorothea DinterStephan LöfflerMaximilian GebhardTilman LaubertAndreas LubienskiHans-Peter BruchAndreas SchmidtBackground/Aim: Ablative techniques such as radiofrequency ablation or non-thermal electrochemical treatment (ECT) are used to manage unresectable liver metastases. Although ECT is not affected by the cooling effect from adjacent vessels, there is a paucity of data available on ECT. Materials and Methods: We used porcine livers to establish an organ model with portal venous and hepatic arterial blood flow for a standardized analysis of the relationship between dose (electric charge) and response (volume of necrosis). Results: This model allowed us to study pressure-controlled perfusion of portal venous and hepatic arterial circulation in the absence of a capillary leak. A specially designed guiding template helped us place platinum electrodes at reproducible locations. With two electrodes, there was a linear relationship between charges of no more than 200 C and necrosis. The relationship was logarithmic at charges of 400-600 C. Larger electrode spacing led to a significant increase in necrosis. We measured pH values of 0.9 (range: 0.6-1.3) at the anode and 12.6 (range: 11.6-13.4) at the cathode. Conclusions: Using a perfusion model, we established an experimental design that allowed us to study ECT in the liver of large animals without experiments on living animals. An electrode template helped us improve the standardized analysis of dose-response relationships. ECT created reproducible and sharply demarcated areas of necrosis, the size of which depended on the charge delivered as well as on the number and spacing of electrodes. Doses higher than 600 C require longer treatment times but do not increase the area of necrosis (logarithmic dose-response relationship).http://www.saudijgastro.com/article.asp?issn=1319-3767;year=2011;volume=17;issue=5;spage=335;epage=342;aulast=CzymekAblationcoulombdose-response relationshipelectrochemical treatmentliverperfusion model |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ralf Czymek Dorothea Dinter Stephan Löffler Maximilian Gebhard Tilman Laubert Andreas Lubienski Hans-Peter Bruch Andreas Schmidt |
spellingShingle |
Ralf Czymek Dorothea Dinter Stephan Löffler Maximilian Gebhard Tilman Laubert Andreas Lubienski Hans-Peter Bruch Andreas Schmidt Electrochemical treatment: An investigation of dose-response relationships using an isolated liver perfusion model The Saudi Journal of Gastroenterology Ablation coulomb dose-response relationship electrochemical treatment liver perfusion model |
author_facet |
Ralf Czymek Dorothea Dinter Stephan Löffler Maximilian Gebhard Tilman Laubert Andreas Lubienski Hans-Peter Bruch Andreas Schmidt |
author_sort |
Ralf Czymek |
title |
Electrochemical treatment: An investigation of dose-response relationships using an isolated liver perfusion model |
title_short |
Electrochemical treatment: An investigation of dose-response relationships using an isolated liver perfusion model |
title_full |
Electrochemical treatment: An investigation of dose-response relationships using an isolated liver perfusion model |
title_fullStr |
Electrochemical treatment: An investigation of dose-response relationships using an isolated liver perfusion model |
title_full_unstemmed |
Electrochemical treatment: An investigation of dose-response relationships using an isolated liver perfusion model |
title_sort |
electrochemical treatment: an investigation of dose-response relationships using an isolated liver perfusion model |
publisher |
Wolters Kluwer Medknow Publications |
series |
The Saudi Journal of Gastroenterology |
issn |
1319-3767 1998-4049 |
publishDate |
2011-01-01 |
description |
Background/Aim: Ablative techniques such as radiofrequency ablation or non-thermal electrochemical treatment (ECT) are used to manage unresectable liver metastases. Although ECT is not affected by the cooling effect from adjacent vessels, there is a paucity of data available on ECT. Materials and Methods: We used porcine livers to establish an organ model with portal venous and hepatic arterial blood flow for a standardized analysis of the relationship between dose (electric charge) and response (volume of necrosis). Results: This model allowed us to study pressure-controlled perfusion of portal venous and hepatic arterial circulation in the absence of a capillary leak. A specially designed guiding template helped us place platinum electrodes at reproducible locations. With two electrodes, there was a linear relationship between charges of no more than 200 C and necrosis. The relationship was logarithmic at charges of 400-600 C. Larger electrode spacing led to a significant increase in necrosis. We measured pH values of 0.9 (range: 0.6-1.3) at the anode and 12.6 (range: 11.6-13.4) at the cathode. Conclusions: Using a perfusion model, we established an experimental design that allowed us to study ECT in the liver of large animals without experiments on living animals. An electrode template helped us improve the standardized analysis of dose-response relationships. ECT created reproducible and sharply demarcated areas of necrosis, the size of which depended on the charge delivered as well as on the number and spacing of electrodes. Doses higher than 600 C require longer treatment times but do not increase the area of necrosis (logarithmic dose-response relationship). |
topic |
Ablation coulomb dose-response relationship electrochemical treatment liver perfusion model |
url |
http://www.saudijgastro.com/article.asp?issn=1319-3767;year=2011;volume=17;issue=5;spage=335;epage=342;aulast=Czymek |
work_keys_str_mv |
AT ralfczymek electrochemicaltreatmentaninvestigationofdoseresponserelationshipsusinganisolatedliverperfusionmodel AT dorotheadinter electrochemicaltreatmentaninvestigationofdoseresponserelationshipsusinganisolatedliverperfusionmodel AT stephanloffler electrochemicaltreatmentaninvestigationofdoseresponserelationshipsusinganisolatedliverperfusionmodel AT maximiliangebhard electrochemicaltreatmentaninvestigationofdoseresponserelationshipsusinganisolatedliverperfusionmodel AT tilmanlaubert electrochemicaltreatmentaninvestigationofdoseresponserelationshipsusinganisolatedliverperfusionmodel AT andreaslubienski electrochemicaltreatmentaninvestigationofdoseresponserelationshipsusinganisolatedliverperfusionmodel AT hanspeterbruch electrochemicaltreatmentaninvestigationofdoseresponserelationshipsusinganisolatedliverperfusionmodel AT andreasschmidt electrochemicaltreatmentaninvestigationofdoseresponserelationshipsusinganisolatedliverperfusionmodel |
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1724975636495204352 |