Deubiquitinating enzyme USP41 promotes lung cancer cell proliferation and migration
Abstract Background To reveal the function of deubiquitylating enzyme USP41 in lung adenocarcinoma. Methods The relationship between USP41 and lung cancer was determined by analyzing data from The Cancer Genome Atlas (TCGA). A549 and H1299 cell lines were transfected with short hairpin RNA against U...
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doaj-b6127907d5fe4f07967a503da1415cfc2021-04-02T04:50:28ZengWileyThoracic Cancer1759-77061759-77142021-04-011271041104710.1111/1759-7714.13843Deubiquitinating enzyme USP41 promotes lung cancer cell proliferation and migrationJiaqi Ji0Shuping Yang1Lingling Zu2Yongwen Li3Ying Li4Department of Pulmonary and Critical Care Medicine Sichuan Academy of Medical Sciences & Sichuan Province People's Hospital Chengdu ChinaDepartment of Anesthesiology Huashan Hospital Affiliated to Fudan University Shanghai ChinaKey Laboratory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Institute Tianjin Medical University General Hospital Tianjin ChinaKey Laboratory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Institute Tianjin Medical University General Hospital Tianjin ChinaKey Laboratory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Institute Tianjin Medical University General Hospital Tianjin ChinaAbstract Background To reveal the function of deubiquitylating enzyme USP41 in lung adenocarcinoma. Methods The relationship between USP41 and lung cancer was determined by analyzing data from The Cancer Genome Atlas (TCGA). A549 and H1299 cell lines were transfected with short hairpin RNA against USP41 (shUSP41 group) or negative control (shCon group). Western blotting was used to verify the transfection efficacy and marker expression. Cell proliferation and apoptosis were analyzed by EdU assay, MTT assay, and flow cytometry after USP41 knockdown. Transwell assay was used to determine the effect of USP41 downregulation on cell migration. Results Analysis of lung cancer data from TCGA database indicated a higher level of USP41 expression in lung cancer tumor tissue compared with that in noncancerous tissue, and USP41 overexpression was correlated with poor overall survival of lung cancer patients (p < 0.01). The outcomes of the EdU, MTT, and flow cytometry assays indicated decreased cell proliferation and enhanced apoptosis in shUSP41‐transfected cells. Transwell assay further demonstrated that USP41 knockdown increased the migration rate of A549 and H1299 cells. Conclusions In our study, USP41 was overexpressed in lung cancer tissue and associated with poor prognosis of lung cancer. USP41 knockdown inhibits cell proliferation and migration and induces cell apoptosis of lung cancer.https://doi.org/10.1111/1759-7714.13843deubiquitylating enzymeinvasionmigrationnon‐small cell lung cancerUSP41 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jiaqi Ji Shuping Yang Lingling Zu Yongwen Li Ying Li |
spellingShingle |
Jiaqi Ji Shuping Yang Lingling Zu Yongwen Li Ying Li Deubiquitinating enzyme USP41 promotes lung cancer cell proliferation and migration Thoracic Cancer deubiquitylating enzyme invasion migration non‐small cell lung cancer USP41 |
author_facet |
Jiaqi Ji Shuping Yang Lingling Zu Yongwen Li Ying Li |
author_sort |
Jiaqi Ji |
title |
Deubiquitinating enzyme USP41 promotes lung cancer cell proliferation and migration |
title_short |
Deubiquitinating enzyme USP41 promotes lung cancer cell proliferation and migration |
title_full |
Deubiquitinating enzyme USP41 promotes lung cancer cell proliferation and migration |
title_fullStr |
Deubiquitinating enzyme USP41 promotes lung cancer cell proliferation and migration |
title_full_unstemmed |
Deubiquitinating enzyme USP41 promotes lung cancer cell proliferation and migration |
title_sort |
deubiquitinating enzyme usp41 promotes lung cancer cell proliferation and migration |
publisher |
Wiley |
series |
Thoracic Cancer |
issn |
1759-7706 1759-7714 |
publishDate |
2021-04-01 |
description |
Abstract Background To reveal the function of deubiquitylating enzyme USP41 in lung adenocarcinoma. Methods The relationship between USP41 and lung cancer was determined by analyzing data from The Cancer Genome Atlas (TCGA). A549 and H1299 cell lines were transfected with short hairpin RNA against USP41 (shUSP41 group) or negative control (shCon group). Western blotting was used to verify the transfection efficacy and marker expression. Cell proliferation and apoptosis were analyzed by EdU assay, MTT assay, and flow cytometry after USP41 knockdown. Transwell assay was used to determine the effect of USP41 downregulation on cell migration. Results Analysis of lung cancer data from TCGA database indicated a higher level of USP41 expression in lung cancer tumor tissue compared with that in noncancerous tissue, and USP41 overexpression was correlated with poor overall survival of lung cancer patients (p < 0.01). The outcomes of the EdU, MTT, and flow cytometry assays indicated decreased cell proliferation and enhanced apoptosis in shUSP41‐transfected cells. Transwell assay further demonstrated that USP41 knockdown increased the migration rate of A549 and H1299 cells. Conclusions In our study, USP41 was overexpressed in lung cancer tissue and associated with poor prognosis of lung cancer. USP41 knockdown inhibits cell proliferation and migration and induces cell apoptosis of lung cancer. |
topic |
deubiquitylating enzyme invasion migration non‐small cell lung cancer USP41 |
url |
https://doi.org/10.1111/1759-7714.13843 |
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