Electrochemical Biosensor for Nitrite Based on Polyacrylic-Graphene Composite Film with Covalently Immobilized Hemoglobin
A new biosensor for the analysis of nitrite in food was developed based on hemoglobin (Hb) covalently immobilized on the succinimide functionalized poly(n-butyl acrylate)-graphene [poly(nBA)-rGO] composite film deposited on a carbon-paste screen-printed electrode (SPE). The immobilized Hb on the pol...
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doaj-b628ed8a3fee4729baddc11b316232582020-11-25T02:27:31ZengMDPI AGSensors1424-82202018-04-01185134310.3390/s18051343s18051343Electrochemical Biosensor for Nitrite Based on Polyacrylic-Graphene Composite Film with Covalently Immobilized HemoglobinRaja Zaidatul Akhmar Raja Jamaluddin0Lee Yook Heng1Ling Ling Tan2Kwok Feng Chong3School of Chemical Sciences and Food Technology, Faculty of Science and Technology, University Kebangsaan Malaysia (UKM), Bangi 43600, Selangor D. E., MalaysiaSchool of Chemical Sciences and Food Technology, Faculty of Science and Technology, University Kebangsaan Malaysia (UKM), Bangi 43600, Selangor D. E., MalaysiaSoutheast Asia Disaster Prevention Research Initiative (SEADPRI-UKM), Institute for Environment and Development (LESTARI), Universiti Kebangsaan Malaysia (UKM), Bangi 43600, Selangor D. E., MalaysiaFaculty of Industrial Sciences & Technology, Universiti Malaysia Pahang, Lebuhraya Tun Razak, Gambang, Kuantan 26300, Pahang Darul Makmur, MalaysiaA new biosensor for the analysis of nitrite in food was developed based on hemoglobin (Hb) covalently immobilized on the succinimide functionalized poly(n-butyl acrylate)-graphene [poly(nBA)-rGO] composite film deposited on a carbon-paste screen-printed electrode (SPE). The immobilized Hb on the poly(nBA)-rGO conducting matrix exhibited electrocatalytic ability for the reduction of nitrite with significant enhancement in the reduction peak at −0.6 V versus Ag/AgCl reference electrode. Thus, direct determination of nitrite can be achieved by monitoring the cathodic peak current signal of the proposed polyacrylic-graphene hybrid film-based voltammetric nitrite biosensor. The nitrite biosensor exhibited a reproducible dynamic linear response range from 0.05–5 mg L−1 nitrite and a detection limit of 0.03 mg L−1. No significant interference was observed by potential interfering ions such as Ca2+, Na+, K+, NH4+, Mg2+, and NO3− ions. Analysis of nitrite in both raw and processed edible bird’s nest (EBN) samples demonstrated recovery of close to 100%. The covalent immobilization of Hb on poly(nBA)-rGO composite film has improved the performance of the electrochemical nitrite biosensor in terms of broader detection range, lower detection limit, and prolonged biosensor stability.http://www.mdpi.com/1424-8220/18/5/1343edible bird’s nestelectrochemical biosensorgraphenehemoglobinnitrite |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Raja Zaidatul Akhmar Raja Jamaluddin Lee Yook Heng Ling Ling Tan Kwok Feng Chong |
spellingShingle |
Raja Zaidatul Akhmar Raja Jamaluddin Lee Yook Heng Ling Ling Tan Kwok Feng Chong Electrochemical Biosensor for Nitrite Based on Polyacrylic-Graphene Composite Film with Covalently Immobilized Hemoglobin Sensors edible bird’s nest electrochemical biosensor graphene hemoglobin nitrite |
author_facet |
Raja Zaidatul Akhmar Raja Jamaluddin Lee Yook Heng Ling Ling Tan Kwok Feng Chong |
author_sort |
Raja Zaidatul Akhmar Raja Jamaluddin |
title |
Electrochemical Biosensor for Nitrite Based on Polyacrylic-Graphene Composite Film with Covalently Immobilized Hemoglobin |
title_short |
Electrochemical Biosensor for Nitrite Based on Polyacrylic-Graphene Composite Film with Covalently Immobilized Hemoglobin |
title_full |
Electrochemical Biosensor for Nitrite Based on Polyacrylic-Graphene Composite Film with Covalently Immobilized Hemoglobin |
title_fullStr |
Electrochemical Biosensor for Nitrite Based on Polyacrylic-Graphene Composite Film with Covalently Immobilized Hemoglobin |
title_full_unstemmed |
Electrochemical Biosensor for Nitrite Based on Polyacrylic-Graphene Composite Film with Covalently Immobilized Hemoglobin |
title_sort |
electrochemical biosensor for nitrite based on polyacrylic-graphene composite film with covalently immobilized hemoglobin |
publisher |
MDPI AG |
series |
Sensors |
issn |
1424-8220 |
publishDate |
2018-04-01 |
description |
A new biosensor for the analysis of nitrite in food was developed based on hemoglobin (Hb) covalently immobilized on the succinimide functionalized poly(n-butyl acrylate)-graphene [poly(nBA)-rGO] composite film deposited on a carbon-paste screen-printed electrode (SPE). The immobilized Hb on the poly(nBA)-rGO conducting matrix exhibited electrocatalytic ability for the reduction of nitrite with significant enhancement in the reduction peak at −0.6 V versus Ag/AgCl reference electrode. Thus, direct determination of nitrite can be achieved by monitoring the cathodic peak current signal of the proposed polyacrylic-graphene hybrid film-based voltammetric nitrite biosensor. The nitrite biosensor exhibited a reproducible dynamic linear response range from 0.05–5 mg L−1 nitrite and a detection limit of 0.03 mg L−1. No significant interference was observed by potential interfering ions such as Ca2+, Na+, K+, NH4+, Mg2+, and NO3− ions. Analysis of nitrite in both raw and processed edible bird’s nest (EBN) samples demonstrated recovery of close to 100%. The covalent immobilization of Hb on poly(nBA)-rGO composite film has improved the performance of the electrochemical nitrite biosensor in terms of broader detection range, lower detection limit, and prolonged biosensor stability. |
topic |
edible bird’s nest electrochemical biosensor graphene hemoglobin nitrite |
url |
http://www.mdpi.com/1424-8220/18/5/1343 |
work_keys_str_mv |
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1724842646044672000 |