Cloning and Functional Verification of Genes Related to 2-Phenylethanol Biosynthesis in <i>Rosa rugosa</i>
In China, <i>Rosa rugosa</i> is cultivated as a source of natural perfumes. Rose essential oil is known as “liquid gold„, given its high economic and health value. 2-phenylethanol accounts for more than 10% of the total mass fraction of the essential oil derived from...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2018-11-01
|
Series: | Genes |
Subjects: | |
Online Access: | https://www.mdpi.com/2073-4425/9/12/576 |
id |
doaj-b6395545342f4e279a00c20187942a91 |
---|---|
record_format |
Article |
spelling |
doaj-b6395545342f4e279a00c20187942a912020-11-25T00:37:29ZengMDPI AGGenes2073-44252018-11-0191257610.3390/genes9120576genes9120576Cloning and Functional Verification of Genes Related to 2-Phenylethanol Biosynthesis in <i>Rosa rugosa</i>Lixia Sheng0Yuqian Zeng1Tiantian Wei2Min Zhu3Xuemin Fang4Xiaoyu Yuan5Yunjian Luo6Liguo Feng7College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, ChinaIn China, <i>Rosa rugosa</i> is cultivated as a source of natural perfumes. Rose essential oil is known as “liquid gold„, given its high economic and health value. 2-phenylethanol accounts for more than 10% of the total mass fraction of the essential oil derived from <i>R. rugosa</i>. The regulatory mechanisms underlying 2-phenylethanol metabolism in <i>R. rugosa</i>, however, remain unclear. In this study, <i>RrAAAT</i> and <i>RrPPDC1</i>, two genes related to 2-phenylethanol synthesis, were cloned from <i>R. rugosa.</i> Expression analysis revealed that <i>RrAAAT</i> and <i>RrPPDC1</i> were highly expressed in rose flowers in the full opening and withering stages, and in calyxes. The overexpression vectors of <i>RrAADC</i>, <i>RrAAAT</i>, and <i>RrPPDC1</i> were established and transformed into <i>Petunia hybrida</i> via <i>Agrobacterium</i>-mediated genetic transformation. Results demonstrated that the overexpression of <i>RrAADC</i> and <i>RrAAAT</i> increased the 2-phenylethanol content of transgenic petunia flowers. The results of this study provide a basis for the introduction of genes related to 2-phenylethanol synthesis into roses to increase the 2-phenylethanol content of rose essential oil.https://www.mdpi.com/2073-4425/9/12/576<i>Rosa rugosa</i>floral scentessential oil2-phenylethanol biosynthesisgene functional verification |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lixia Sheng Yuqian Zeng Tiantian Wei Min Zhu Xuemin Fang Xiaoyu Yuan Yunjian Luo Liguo Feng |
spellingShingle |
Lixia Sheng Yuqian Zeng Tiantian Wei Min Zhu Xuemin Fang Xiaoyu Yuan Yunjian Luo Liguo Feng Cloning and Functional Verification of Genes Related to 2-Phenylethanol Biosynthesis in <i>Rosa rugosa</i> Genes <i>Rosa rugosa</i> floral scent essential oil 2-phenylethanol biosynthesis gene functional verification |
author_facet |
Lixia Sheng Yuqian Zeng Tiantian Wei Min Zhu Xuemin Fang Xiaoyu Yuan Yunjian Luo Liguo Feng |
author_sort |
Lixia Sheng |
title |
Cloning and Functional Verification of Genes Related to 2-Phenylethanol Biosynthesis in <i>Rosa rugosa</i> |
title_short |
Cloning and Functional Verification of Genes Related to 2-Phenylethanol Biosynthesis in <i>Rosa rugosa</i> |
title_full |
Cloning and Functional Verification of Genes Related to 2-Phenylethanol Biosynthesis in <i>Rosa rugosa</i> |
title_fullStr |
Cloning and Functional Verification of Genes Related to 2-Phenylethanol Biosynthesis in <i>Rosa rugosa</i> |
title_full_unstemmed |
Cloning and Functional Verification of Genes Related to 2-Phenylethanol Biosynthesis in <i>Rosa rugosa</i> |
title_sort |
cloning and functional verification of genes related to 2-phenylethanol biosynthesis in <i>rosa rugosa</i> |
publisher |
MDPI AG |
series |
Genes |
issn |
2073-4425 |
publishDate |
2018-11-01 |
description |
In China, <i>Rosa rugosa</i> is cultivated as a source of natural perfumes. Rose essential oil is known as “liquid gold„, given its high economic and health value. 2-phenylethanol accounts for more than 10% of the total mass fraction of the essential oil derived from <i>R. rugosa</i>. The regulatory mechanisms underlying 2-phenylethanol metabolism in <i>R. rugosa</i>, however, remain unclear. In this study, <i>RrAAAT</i> and <i>RrPPDC1</i>, two genes related to 2-phenylethanol synthesis, were cloned from <i>R. rugosa.</i> Expression analysis revealed that <i>RrAAAT</i> and <i>RrPPDC1</i> were highly expressed in rose flowers in the full opening and withering stages, and in calyxes. The overexpression vectors of <i>RrAADC</i>, <i>RrAAAT</i>, and <i>RrPPDC1</i> were established and transformed into <i>Petunia hybrida</i> via <i>Agrobacterium</i>-mediated genetic transformation. Results demonstrated that the overexpression of <i>RrAADC</i> and <i>RrAAAT</i> increased the 2-phenylethanol content of transgenic petunia flowers. The results of this study provide a basis for the introduction of genes related to 2-phenylethanol synthesis into roses to increase the 2-phenylethanol content of rose essential oil. |
topic |
<i>Rosa rugosa</i> floral scent essential oil 2-phenylethanol biosynthesis gene functional verification |
url |
https://www.mdpi.com/2073-4425/9/12/576 |
work_keys_str_mv |
AT lixiasheng cloningandfunctionalverificationofgenesrelatedto2phenylethanolbiosynthesisinirosarugosai AT yuqianzeng cloningandfunctionalverificationofgenesrelatedto2phenylethanolbiosynthesisinirosarugosai AT tiantianwei cloningandfunctionalverificationofgenesrelatedto2phenylethanolbiosynthesisinirosarugosai AT minzhu cloningandfunctionalverificationofgenesrelatedto2phenylethanolbiosynthesisinirosarugosai AT xueminfang cloningandfunctionalverificationofgenesrelatedto2phenylethanolbiosynthesisinirosarugosai AT xiaoyuyuan cloningandfunctionalverificationofgenesrelatedto2phenylethanolbiosynthesisinirosarugosai AT yunjianluo cloningandfunctionalverificationofgenesrelatedto2phenylethanolbiosynthesisinirosarugosai AT liguofeng cloningandfunctionalverificationofgenesrelatedto2phenylethanolbiosynthesisinirosarugosai |
_version_ |
1725301080780177408 |