A laboratory-based study to explore the use of honey-impregnated cards to detect chikungunya virus in mosquito saliva.
Mosquito control is implemented when arboviruses are detected in patients or in field-collected mosquitoes. However, mass screening of mosquitoes is usually laborious and expensive, requiring specialized expertise and equipment. Detection of virus in mosquito saliva using honey-impregnated filter pa...
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2021-01-01
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Online Access: | https://doi.org/10.1371/journal.pone.0249471 |
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doaj-b670ba6ad0b34ae7952e1e6222e532f02021-04-11T04:30:22ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-01164e024947110.1371/journal.pone.0249471A laboratory-based study to explore the use of honey-impregnated cards to detect chikungunya virus in mosquito saliva.Lisa FourniolYoann MadecLaurence MoussonMarie VazeilleAnna-Bella FaillouxMosquito control is implemented when arboviruses are detected in patients or in field-collected mosquitoes. However, mass screening of mosquitoes is usually laborious and expensive, requiring specialized expertise and equipment. Detection of virus in mosquito saliva using honey-impregnated filter papers seems to be a promising method as it is non-destructive and allows monitoring the viral excretion dynamics over time from the same mosquito. Here we test the use of filter papers to detect chikungunya virus in mosquito saliva in laboratory conditions, before proposing this method in large-scale mosquito surveillance programs. We found that 0.9 cm2 cards impregnated with a 50% honey solution could replace the forced salivation technique as they offered a viral RNA detection until 7 days after oral infection of Aedes aegypti and Aedes albopictus mosquitoes with CHIKV.https://doi.org/10.1371/journal.pone.0249471 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lisa Fourniol Yoann Madec Laurence Mousson Marie Vazeille Anna-Bella Failloux |
spellingShingle |
Lisa Fourniol Yoann Madec Laurence Mousson Marie Vazeille Anna-Bella Failloux A laboratory-based study to explore the use of honey-impregnated cards to detect chikungunya virus in mosquito saliva. PLoS ONE |
author_facet |
Lisa Fourniol Yoann Madec Laurence Mousson Marie Vazeille Anna-Bella Failloux |
author_sort |
Lisa Fourniol |
title |
A laboratory-based study to explore the use of honey-impregnated cards to detect chikungunya virus in mosquito saliva. |
title_short |
A laboratory-based study to explore the use of honey-impregnated cards to detect chikungunya virus in mosquito saliva. |
title_full |
A laboratory-based study to explore the use of honey-impregnated cards to detect chikungunya virus in mosquito saliva. |
title_fullStr |
A laboratory-based study to explore the use of honey-impregnated cards to detect chikungunya virus in mosquito saliva. |
title_full_unstemmed |
A laboratory-based study to explore the use of honey-impregnated cards to detect chikungunya virus in mosquito saliva. |
title_sort |
laboratory-based study to explore the use of honey-impregnated cards to detect chikungunya virus in mosquito saliva. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2021-01-01 |
description |
Mosquito control is implemented when arboviruses are detected in patients or in field-collected mosquitoes. However, mass screening of mosquitoes is usually laborious and expensive, requiring specialized expertise and equipment. Detection of virus in mosquito saliva using honey-impregnated filter papers seems to be a promising method as it is non-destructive and allows monitoring the viral excretion dynamics over time from the same mosquito. Here we test the use of filter papers to detect chikungunya virus in mosquito saliva in laboratory conditions, before proposing this method in large-scale mosquito surveillance programs. We found that 0.9 cm2 cards impregnated with a 50% honey solution could replace the forced salivation technique as they offered a viral RNA detection until 7 days after oral infection of Aedes aegypti and Aedes albopictus mosquitoes with CHIKV. |
url |
https://doi.org/10.1371/journal.pone.0249471 |
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