Serotonin uptake via plasma membrane monoamine transporter during myocardial ischemia‐reperfusion in the rat heart in vivo
Abstract Serotonin (5‐HT) accumulates in the heart during myocardial ischemia and induces deleterious effects on the cardiomyocytes through receptor‐dependent and monoamine oxidase‐dependent pathways. We aimed to clarify the involvement of extra‐neuronal monoamine transporters in the clearance of 5‐...
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Online Access: | https://doi.org/10.14814/phy2.14297 |
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doaj-b67ebee1854d4b2b8d50bdff0588a9ed2020-11-25T03:04:36ZengWileyPhysiological Reports2051-817X2019-11-01722n/an/a10.14814/phy2.14297Serotonin uptake via plasma membrane monoamine transporter during myocardial ischemia‐reperfusion in the rat heart in vivoTakashi Sonobe0Tsuyoshi Akiyama1Cheng‐Kun Du2James T. Pearson3Department of Cardiac Physiology National Cerebral and Cardiovascular Center Research Institute Suita Osaka JapanDepartment of Cardiac Physiology National Cerebral and Cardiovascular Center Research Institute Suita Osaka JapanDepartment of Cardiac Physiology National Cerebral and Cardiovascular Center Research Institute Suita Osaka JapanDepartment of Cardiac Physiology National Cerebral and Cardiovascular Center Research Institute Suita Osaka JapanAbstract Serotonin (5‐HT) accumulates in the heart during myocardial ischemia and induces deleterious effects on the cardiomyocytes through receptor‐dependent and monoamine oxidase‐dependent pathways. We aimed to clarify the involvement of extra‐neuronal monoamine transporters in the clearance of 5‐HT during ischemia and reperfusion in the heart. Using a microdialysis technique in the anesthetized Wistar rat heart, we monitored myocardial interstitial 5‐HT and 5‐hydroxyindole acetic acid (5‐HIAA) concentration by means of electro‐chemical detection coupled with high‐performance liquid chromatography (HPLC‐ECD). Effects of inhibitors of the plasma membrane monoamine transporter (PMAT) and the organic cation transporter 3 (OCT3) (decynium‐22 and corticosterone) on the 5‐HT and 5‐HIAA concentrations during baseline, coronary occlusion, and reperfusion were investigated. Basal dialysate 5‐HT concentration were increased by local administration of decynium‐22, but not by corticosterone. Addition of fluoxetine, a serotonin transporter (SERT) inhibitor further increased the 5‐HT concentration upon during administration of decynium‐22. Decynium‐22 elevated the background level of 5‐HT during coronary occlusion and maintained 5‐HT concentration at a high level during reperfusion. Production of 5‐HIAA in the early reperfusion was significantly suppressed by decynium‐22. These results indicate that PMAT and SERT independently regulate basal level of interstitial 5‐HT, and PMAT plays a more important role in the clearance of 5‐HT during reperfusion. These data suggest the involvement of PMAT in the monoamine oxidase‐dependent deleterious pathway in the heart.https://doi.org/10.14814/phy2.142975‐HTin vivo cardiac microdialysisischemia reperfusion injuryorganic cation transporterplasma membrane monoamine transporterserotonin transporter |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Takashi Sonobe Tsuyoshi Akiyama Cheng‐Kun Du James T. Pearson |
spellingShingle |
Takashi Sonobe Tsuyoshi Akiyama Cheng‐Kun Du James T. Pearson Serotonin uptake via plasma membrane monoamine transporter during myocardial ischemia‐reperfusion in the rat heart in vivo Physiological Reports 5‐HT in vivo cardiac microdialysis ischemia reperfusion injury organic cation transporter plasma membrane monoamine transporter serotonin transporter |
author_facet |
Takashi Sonobe Tsuyoshi Akiyama Cheng‐Kun Du James T. Pearson |
author_sort |
Takashi Sonobe |
title |
Serotonin uptake via plasma membrane monoamine transporter during myocardial ischemia‐reperfusion in the rat heart in vivo |
title_short |
Serotonin uptake via plasma membrane monoamine transporter during myocardial ischemia‐reperfusion in the rat heart in vivo |
title_full |
Serotonin uptake via plasma membrane monoamine transporter during myocardial ischemia‐reperfusion in the rat heart in vivo |
title_fullStr |
Serotonin uptake via plasma membrane monoamine transporter during myocardial ischemia‐reperfusion in the rat heart in vivo |
title_full_unstemmed |
Serotonin uptake via plasma membrane monoamine transporter during myocardial ischemia‐reperfusion in the rat heart in vivo |
title_sort |
serotonin uptake via plasma membrane monoamine transporter during myocardial ischemia‐reperfusion in the rat heart in vivo |
publisher |
Wiley |
series |
Physiological Reports |
issn |
2051-817X |
publishDate |
2019-11-01 |
description |
Abstract Serotonin (5‐HT) accumulates in the heart during myocardial ischemia and induces deleterious effects on the cardiomyocytes through receptor‐dependent and monoamine oxidase‐dependent pathways. We aimed to clarify the involvement of extra‐neuronal monoamine transporters in the clearance of 5‐HT during ischemia and reperfusion in the heart. Using a microdialysis technique in the anesthetized Wistar rat heart, we monitored myocardial interstitial 5‐HT and 5‐hydroxyindole acetic acid (5‐HIAA) concentration by means of electro‐chemical detection coupled with high‐performance liquid chromatography (HPLC‐ECD). Effects of inhibitors of the plasma membrane monoamine transporter (PMAT) and the organic cation transporter 3 (OCT3) (decynium‐22 and corticosterone) on the 5‐HT and 5‐HIAA concentrations during baseline, coronary occlusion, and reperfusion were investigated. Basal dialysate 5‐HT concentration were increased by local administration of decynium‐22, but not by corticosterone. Addition of fluoxetine, a serotonin transporter (SERT) inhibitor further increased the 5‐HT concentration upon during administration of decynium‐22. Decynium‐22 elevated the background level of 5‐HT during coronary occlusion and maintained 5‐HT concentration at a high level during reperfusion. Production of 5‐HIAA in the early reperfusion was significantly suppressed by decynium‐22. These results indicate that PMAT and SERT independently regulate basal level of interstitial 5‐HT, and PMAT plays a more important role in the clearance of 5‐HT during reperfusion. These data suggest the involvement of PMAT in the monoamine oxidase‐dependent deleterious pathway in the heart. |
topic |
5‐HT in vivo cardiac microdialysis ischemia reperfusion injury organic cation transporter plasma membrane monoamine transporter serotonin transporter |
url |
https://doi.org/10.14814/phy2.14297 |
work_keys_str_mv |
AT takashisonobe serotoninuptakeviaplasmamembranemonoaminetransporterduringmyocardialischemiareperfusionintheratheartinvivo AT tsuyoshiakiyama serotoninuptakeviaplasmamembranemonoaminetransporterduringmyocardialischemiareperfusionintheratheartinvivo AT chengkundu serotoninuptakeviaplasmamembranemonoaminetransporterduringmyocardialischemiareperfusionintheratheartinvivo AT jamestpearson serotoninuptakeviaplasmamembranemonoaminetransporterduringmyocardialischemiareperfusionintheratheartinvivo |
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